A study was done to investigate the incidence of Brucella abortus in cattle and buffaloes in Thatta Sindh. A total of n = 360 serum samples were randomly collected from buffaloes and cattle (130 each species). The Rose Bengal Plate Test was used to screen serum samples at first (RBPT). A B. abortus specific indirect enzyme-linked immunosorbent test was performed on RBPT positive samples (i-ELISA). An rPCR was used to investigate the efficacy of detecting Brucella in the blood of infected animals after serum samples were proven to be positive for B. abortus by serology. The effectiveness of an rPCR reported in detecting Brucella at the genus level and later at the species level (B. abortus and B. melitensis) in the serum of sick cattle and buffaloes was investigated. The samples that were verified to be positive via both immunological tests, RBPT and i-ELISA, were submitted to the rPCR for this reason. Initially, rPCR based on the Brucella genus-specific bcsp31 genomic region was utilized. The IS711 genomic region of B. abortus and B. melitensis was discovered using two species-specific rPCRs. By RBPT, 13 serum samples from cattle (10%) and 3 from buffalo (2.31%) were shown to be positive for B. abortus. 8 (6.15%) of the 13 RBPT positive cattle samples also tested positive in i-ELISA, whereas 5 tested negative. The 3 buffalo that tested positive for RBPT then 2 were tested positive for i-ELISA. All 8 seropositive samples had Brucella genus specific rPCR amplification. B. abortus was found in all of the samples using species-specific rPCR.
| Published in | Advances in Bioscience and Bioengineering (Volume 9, Issue 4) |
| DOI | 10.11648/j.abb.20210904.11 |
| Page(s) | 92-95 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Bovine Brucellosis, ELISA, Sindh, RBPT, rPCR
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APA Style
Abdullah Babar, Adnan Yousaf, Inayatullah Sarki, Asghar Subhani. (2021). Incidence of Bovine Brucellosis in Thatta, Sindh-Pakistan. Advances in Bioscience and Bioengineering, 9(4), 92-95. https://doi.org/10.11648/j.abb.20210904.11
ACS Style
Abdullah Babar; Adnan Yousaf; Inayatullah Sarki; Asghar Subhani. Incidence of Bovine Brucellosis in Thatta, Sindh-Pakistan. Adv. BioSci. Bioeng. 2021, 9(4), 92-95. doi: 10.11648/j.abb.20210904.11
AMA Style
Abdullah Babar, Adnan Yousaf, Inayatullah Sarki, Asghar Subhani. Incidence of Bovine Brucellosis in Thatta, Sindh-Pakistan. Adv BioSci Bioeng. 2021;9(4):92-95. doi: 10.11648/j.abb.20210904.11
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Download@article{10.11648/j.abb.20210904.11,
author = {Abdullah Babar and Adnan Yousaf and Inayatullah Sarki and Asghar Subhani},
title = {Incidence of Bovine Brucellosis in Thatta, Sindh-Pakistan},
journal = {Advances in Bioscience and Bioengineering},
volume = {9},
number = {4},
pages = {92-95},
doi = {10.11648/j.abb.20210904.11},
url = {https://doi.org/10.11648/j.abb.20210904.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210904.11},
abstract = {A study was done to investigate the incidence of Brucella abortus in cattle and buffaloes in Thatta Sindh. A total of n = 360 serum samples were randomly collected from buffaloes and cattle (130 each species). The Rose Bengal Plate Test was used to screen serum samples at first (RBPT). A B. abortus specific indirect enzyme-linked immunosorbent test was performed on RBPT positive samples (i-ELISA). An rPCR was used to investigate the efficacy of detecting Brucella in the blood of infected animals after serum samples were proven to be positive for B. abortus by serology. The effectiveness of an rPCR reported in detecting Brucella at the genus level and later at the species level (B. abortus and B. melitensis) in the serum of sick cattle and buffaloes was investigated. The samples that were verified to be positive via both immunological tests, RBPT and i-ELISA, were submitted to the rPCR for this reason. Initially, rPCR based on the Brucella genus-specific bcsp31 genomic region was utilized. The IS711 genomic region of B. abortus and B. melitensis was discovered using two species-specific rPCRs. By RBPT, 13 serum samples from cattle (10%) and 3 from buffalo (2.31%) were shown to be positive for B. abortus. 8 (6.15%) of the 13 RBPT positive cattle samples also tested positive in i-ELISA, whereas 5 tested negative. The 3 buffalo that tested positive for RBPT then 2 were tested positive for i-ELISA. All 8 seropositive samples had Brucella genus specific rPCR amplification. B. abortus was found in all of the samples using species-specific rPCR.},
year = {2021}
}
TY - JOUR T1 - Incidence of Bovine Brucellosis in Thatta, Sindh-Pakistan AU - Abdullah Babar AU - Adnan Yousaf AU - Inayatullah Sarki AU - Asghar Subhani Y1 - 2021/11/05 PY - 2021 N1 - https://doi.org/10.11648/j.abb.20210904.11 DO - 10.11648/j.abb.20210904.11 T2 - Advances in Bioscience and Bioengineering JF - Advances in Bioscience and Bioengineering JO - Advances in Bioscience and Bioengineering SP - 92 EP - 95 PB - Science Publishing Group SN - 2330-4162 UR - https://doi.org/10.11648/j.abb.20210904.11 AB - A study was done to investigate the incidence of Brucella abortus in cattle and buffaloes in Thatta Sindh. A total of n = 360 serum samples were randomly collected from buffaloes and cattle (130 each species). The Rose Bengal Plate Test was used to screen serum samples at first (RBPT). A B. abortus specific indirect enzyme-linked immunosorbent test was performed on RBPT positive samples (i-ELISA). An rPCR was used to investigate the efficacy of detecting Brucella in the blood of infected animals after serum samples were proven to be positive for B. abortus by serology. The effectiveness of an rPCR reported in detecting Brucella at the genus level and later at the species level (B. abortus and B. melitensis) in the serum of sick cattle and buffaloes was investigated. The samples that were verified to be positive via both immunological tests, RBPT and i-ELISA, were submitted to the rPCR for this reason. Initially, rPCR based on the Brucella genus-specific bcsp31 genomic region was utilized. The IS711 genomic region of B. abortus and B. melitensis was discovered using two species-specific rPCRs. By RBPT, 13 serum samples from cattle (10%) and 3 from buffalo (2.31%) were shown to be positive for B. abortus. 8 (6.15%) of the 13 RBPT positive cattle samples also tested positive in i-ELISA, whereas 5 tested negative. The 3 buffalo that tested positive for RBPT then 2 were tested positive for i-ELISA. All 8 seropositive samples had Brucella genus specific rPCR amplification. B. abortus was found in all of the samples using species-specific rPCR. VL - 9 IS - 4 ER -
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