| Peer-Reviewed

Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures

Received: 17 May 2021    Accepted: 29 May 2021    Published: 21 June 2021
Views:       Downloads:
Abstract

A washing-disinfectant product was synthesized with citric acid which was a readily available raw material on the chemical markets. Following-up the established procedure to use this product reflected its efficiency to eliminate, to remove, to kill and to decrease significantly the binary fission of various germs and micro-organisms not only on raw materials but also on materials and containers that could cause consumers illness. Indeed, the quantifications of these micro-organisms on a product “spicy product energy booster (speb)” – “produit épicé boosteur d’énergie (pebe)” at the “Institut Pasteur de Madagascar” whose analyzes were accredited by COFRAC © reflected that firstly there were any Salmonella spp. and any Listeria monocytogenes; secondly its quantities of Bacillus cereus, Escherichia coli and Staphylococcus aureus respected the European standardization for food products; thirdly this “speb-pebe” product decreased significantly the binary fission speed of these previous micro-organisms in order 4.7 to 80 times less speed than its speeds deduced by a reference and/or by its generation time; and fourth the product “speb-pebe” best-before dates during a conservation at 303.15 (°K), natural ambient temperature, were calculated from each micro-organism’s binary fission speed. Then, two procedures titrations consisting in titration of the “speb-pebe” - spicy product energy booster’s equivalent-citric acid molecules in each level of its packaging using NaOH-0.0557N and in titration of the “speb-pebe” - spicy product energy booster’s alkenes-C= organic functions (on surface and on structure-texture) in each level of its packaging using HF-0.0026N were established and allowed to appreciate the “speb-pebe” quality in each level of its packaging and also to control its packaging-quality. It was noticed that the concentration of alkenes-C= organic functions on structure and on texture per grams on the Level-high-packaging were 5.9 times less than those on Level-medium-packaging 1.23E-1 [mol×l-1×g-1] and on Level-low-packaging 1.16E-1 [mol×l-1×g-1] confirming in return the highest value of the concentration of alkenes-C= organic functions on surface per grams of “speb-pebe” observed at Level-high-packaging sample (4.805E-3 [mol×l-1×g-1]) compared with Level-middle-packaging sample (1.72E-3 [mol×l-1×g-1]) and with Level-low-packaging sample (2.19E-3 [mol×l-1×g-1]). Another results, the dispersion of the equivalent-citric acids molecules on total alkenes-C= organic functions noted dispersion (Ac⁄C=) or dispersion ([Ac]⁄[C=]) for the Level-high-packaging sample, the Level-middle-packaging sample and the Level-low-packaging sample were respectively 0.2813, 0.0250 and 0.0714 and confirmed the useful role of citric acids molecules quantities and their protons H+ capacities and activities to catalyze some chemistry reactions and responsible of this “speb-pebe” - spicy product energy booster’s acidity-pH=2.62, flavors and tastes.

Published in American Journal of Applied Chemistry (Volume 9, Issue 3)
DOI 10.11648/j.ajac.20210903.11
Page(s) 53-64
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Micro-organisms, Disinfectant, Binary Fission Speed, Citric Acid, “Speb-Pebe”, NaOH, HF, Alkenes-C=

References
[1] «Sterilization by dry heat» E. M. Darmady, K. E. A. Hughes, J. D. Jones, D. Prince, And Winifred Tuke, J. clin. Path,‎ 1961, p. 7.
[2] «Stérilisation – Traitement des aliments à haute température» - https://www.espritsante.com/articles/sterilisation-traitement-aliments-hautes-temperatures
[3] Brown AC (2007). “Understanding Food: Principles and Preparation (3 ed.)”. Cengage Learning. p. 546. ISBN 978-0-495-10745-3.
[4] Diehl JR (March 2002). "Food irradiation—past, present and future". Radiation Physics and Chemistry. 63 (3–6): 211–215. Bibcode: 2002RaPC...63..211D.
[5] Molins RA (2001). Food irradiation: principles and applications. Wiley-IEEE. p. 23.
[6] "Guidelines for Disinfection" (PDF). Centers for Disease Control. 2008.
[7] "Guidance for Industry, Sterile Drug Products Produced by Aseptic Processing — Current Good Manufacturing Practice" (PDF). U.S. Department of Health and Human Services. 2004.
[8] Onyango LA, Dunstan RH, Roberts TK (May 2010). "Filterability of staphylococcal species through membrane filters following application of stressors". BMC Research Notes. 3: 152
[9] Ir Murielle HELLEPUTTE (Département Agro-nutriton Celabor) – Dr Georges DAUBE (Faculté de Médecine Vétérinaire – Département des Sciences des Denrées Alimentaires. Fundamental and Applied Research for Animal & Health (Farah) – «Comment sont établies les dates de pérempton: qualité du produit et législation».
[10] Le Gouvernement du Grand-Duché de Luxembourg-Ministère de la santé-Direction de la santé: «Critères microbiologiques applicables aux denrées alimentaires Lignes directrices pour l’interprétation» Edition Août 2018.
[11] Rabeharitsara Andry Tahina, Raharilaza Paulin Merix, Randriana Nambinina Richard: “Esterification Between Citric acid and Pumpkin Pips’ Organic Molecules – Esters Hydrolysis And Esters Used as Hydrocarbons Additives”, American Journal of Applied Chemistry - Volume 6, Issue 3, June 2018, Pages: 78-95 - Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar.
[12] Rabeharitsara Andry Tahina, Sedraniaina Domoina Marie Esperance, Randriamanantena Ny Idealy Elite, Mampitefa Raïssa Faneva, Randriana Nambinina Richard: “Esterification Between Citric Acid and Callistemon citrinus, Rice-Husk, Garcinia dulcis Catalysed by Citric Acid’s-H+- Monomers and Polymers Formation Mechanism”, American Journal of Applied Chemistry - Volume 8, Issue 2, April 2020, Pages: 31-54 - Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar.
[13] Rabeharitsara Andry Tahina, Rovatahianjanahary Behevitra, Randriana Nambinina Richard: “Pine Wood Powder Treatment to BXH+ Homogeneous Catalyst (H+/H2SO4) Supported on Its Aromatics’ and PNA’ Alkenes – Application in Black Citric Acid Polymer Synthesis”, American Journal of Polymer Science and Technology - Volume 4, Issue 1, March 2018, Pages: 1-27 - Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar.
[14] Goldman, Emanuel; Green, Lorrence H (24 August 2008). Practical Handbook of Microbiology, Second Edition (Google eBook) (Second ed.). USA: CRC Press, Taylor and Francis Group. p. 864. ISBN 978-0-8493-9365-5. Retrieved 2014-10-16.
[15] Breed RS, Dotterrer WD (May 1916). "The Number of Colonies Allowable on Satisfactory Agar Plates". Journal of Bacteriology. 1 (3): 321–31. doi: 10.1128/JB.1.3.321-331.1916. PMC 378655. PMID 16558698.
[16] Schug, Angela R.; Bartel, Alexander; Meurer, Marita; Scholtzek, Anissa D.; Brombach, Julian; Hensel, Vivian; Fanning, Séamus; Schwarz, Stefan; Feßler, Andrea T. (2020-12-01). "Comparison of two methods for cell count determination in the course of biocide susceptibility testing". Veterinary Microbiology. 251: 108831. doi: 10.1016/j.vetmic.2020.108831.
[17] Salmonellose: symptômes et traitements de la salmonellose - https://www.passeportsante.net/fr/Maux/Problemes/Fiche.aspx?doc=salmonellose_pm
[18] Salmonella — Wikipédia - https://fr.wikipedia.org/wiki/Salmonella
[19] Marc MORTUREUX - «Avis de l’Agence française de sécurité sanitaire des aliments relatif aux conséquences sur les flores microbiennes d’une réduction en taux de sel dans les aliments» - Afssa – Saisine n° 2008-SA-0173 - Maisons-Alfort, le 31 mai 2010 - https://www.anses.fr/fr/system/files/MIC2008sa0173.pdf.
[20] Ellin Doyle, «Virulence characteristics of Listeria monocytogenes» [archive], Food Research Institut, University of Wisconsin-Madison, 2011
[21] Bremer, Phil (3 October 2016). "Bacillus spores in the food industry: A review on resistance and response to novel inactivation technologies". Comprehensive Reviews in Food Science and Food Safety. 15 (2016): 1139–1148. doi: 10.1111/1541-4337.12231. PMID 33401831
[22] Mikkola, Raimo (2006). Food and indoor air isolated Bacillus non-protein toxins: structures, physico-chemical properties and mechanisms of effects on eukaryotic cells (PDF) (Thesis). University of Helsinki. p. 12. ISBN 952-10-3549-8. Archived (PDF) from the original on 9 July 2019. Retrieved 24 October 2015 - https://en.wikipedia.org/wiki/Bacillus_cereus#cite_note-bremer-28
[23] International Dairy Federation “Bacillus cereus in milk and dairy products” IDF Factsheet – December 2016 - https://www.fil-idf.org/wp-content/uploads/2016/12/Bacillus-cereus-in-Milk-and-Dairy-Products.pdf
[24] Agence Nationale de Sécurité Sanitaire alimentation, environnement, travail (anses) – «Bacillus cereus» – Famille des Bacillaceae – Genre Bacillus – Bactérie. https://www.anses.fr/fr/system/files/MIC2011sa0116Fi.pdf
[25] Don J. Brenner; Noel R. Krieg; James T. Staley (July 26, 2005) [1984 (Williams & Wilkins)]. George M. Garrity (ed.). The Gammaproteobacteria. Bergey's Manual of Systematic Bacteriology. 2B (2nd ed.). New York: Springer. p. 1108. ISBN 978-0-387-24144-9. British Library no. GBA561951.
[26] J. F. Rigarlsford: “Microbiological monitoring of cleaning and disinfection in food plants” in Microbiological Analysis of Red Meat, Poultry and Eggs, 2007 - Woodhead Publishing Series in Food Science, Technology and Nutrition 2007, Pages 165-182.
[27] Lowy FD: Staphylococcus aureus infections. N Engl J Med 1998; 339: 520–532.
[28] Molly McAdow Dominique M. Missiakas Olaf Schneewind: ” Staphylococcus aureus Secretes Coagulase and von Willebrand Factor Binding Protein to Modify the Coagulation Cascade and Establish Host Infections” – Journal of innate immunity 2012; 4: 141-148 - DOI: 10.1159/000333447 - Department of Microbiology, University of Chicago, Chicago, Ill., USA.
[29] Kannan, Muthukumar; Riekhof, Wayne R.; Voelker, Dennis R. (2015). "Transport of Phosphatidylserine from the Endoplasmic Reticulum to the Site of Phosphatidylserine Decarboxylase2 in Yeast". Traffic. 16 (2): 123–134. doi: 10.1111/tra.12236. ISSN 1600-0854. PMID 25355612.
[30] Carole Paufique - «L'acide hyaluronique, la molécule star pour une peau repulpée» https://www.marieclaire.fr/,acide-hyaluronique,20145,516640.asp
[31] Stern, Robert, ed. (2009). Hyaluronan in cancer biology (1st ed.). San Diego, CA: Academic Press/Elsevier. ISBN 978-0-12-374178-3 - https://en.wikipedia.org/wiki/Hyaluronic_acid
[32] King, John V.; Liang, Wenguang G.; Scherpelz, Kathryn P.; Schilling, Alexander B.; Meredith, Stephen C.; Tang, Wei-Jen (2014-07-08). "Molecular basis of substrate recognition and degradation by human presequence protease". Structure. 22 (7): 996–1007. doi: 10.1016/j.str.2014.05.003. ISSN 1878-4186. PMC 4128088. PMID 24931469.
[33] P K Nigam and Anjana Nigam: “Botulinum Toxin” Indian Journal of Dermatology” - 2010 Jan-Mar; 55 (1): 8–14 doi: 10.4103/0019-5154.60343 PMCID: PMC2856357 PMID: 20418969
[34] Münchau A, Bhatia KP. “Uses of botulinum toxin injection in medicine today”. BMJ. 2000; 320: 161–5.
[35] B Monti, M Sparapani, A Contestabile: “Differential toxicity of protease inhibitors in cultures of cerebellar granule neurons” – National Library of Medecine (National Center for Biotechnology Information) - Exp Neurol 1998 Oct; 153 (2): 335-41. doi: 10.1006/exnr.1998.6858.
[36] Rabeharitsara Andry Tahina, Raherimandimby Jaochim, Randriana Nambinina Richard: “Trans-Esterification Between Citric Acid and Peanut Oil at Low pH and Ambient Temperature Catalyzed by Citric Acid and Sulfuric Acid Protonic Acid-H+”, American Journal of Applied Chemistry - Volume 8, Issue 4, August 2020, Pages: 100-116 - Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar.
[37] Burkhard Malorny, Charlotta Löfström, Martin Wagner, Nadine Krämer, Jeffrey Hoorfar: “Enumeration of Salmonella Bacteria in Food and Feed Samples by Real-Time PCR for Quantitative Microbial Risk Assessment”, American Society for Microbiology – Applied and Environmental Microbiology. doi: 10.1128/AEM.02489-07.
[38] (CE) No2073/2005 DE LA COMMISSION du 15 novembre 2005 concernant les critères microbiologiques applicables aux denrées alimentaires (Texte présentant de l'intérêt pour l'EEE) (JO L 338 du 22.12.2005, p. 1).
[39] Robert Jarret, Terry Berke, Elizabeth A Baldwin, George Antonious: “Variability for Free Sugars and Organic Acids in Capsicum chinense” - March 2009, Chemistry & Biodiversity 6 (2): 138-45, doi: 10.1002/cbdv.200800046.
[40] Andrews, J., 1984. Peppers: the domesticated Capsicums. University of Texas Press, Austin, Texas, United States. 170 pp.
[41] Bosland, P. W. & Votava, E. J., 2000. Peppers: vegetable and spice capsicums. CABI Publishing, Wallingford, United Kingdom. 204 pp.
[42] Burkill, H. M., 2000. The useful plants of West Tropical Africa. 2nd Edition. Volume 5, Families S–Z, Addenda. Royal Botanic Gardens, Kew, Richmond, United Kingdom. 686 pp.
[43] Heiser, C. B. & Smith, P. G., 1953. The cultivated Capsicum peppers. Economic Botany 7 (3): 214–227.
[44] Messiaen, C.-M., 1989. Le potager tropical. 2nd Edition. Presses Universitaires de France, Paris, France. 580 pp.
[45] Poulos, J. M., 1993. Capsicum L. In: Siemonsma, J. S. & Kasem Piluek (Editors). Plant Resources of South-East Asia No 8. Vegetables. Pudoc Scientific Publishers, Wageningen, Netherlands. pp. 136–140.
[46] Poulos, J. M., 1994. Pepper breeding (Capsicum spp.): achievements, challenges and possibilities. Plant Breeding Abstracts 64 (2): 143–155.
[47] Jansen, P. C. M., 1981. Spices, condiments and medicinal plants in Ethiopia, their taxonomy and agricultural significance. Agricultural Research Reports 906. Centre for Agricultural Publishing and Documentation, Wageningen, Netherlands. 327 pp.
[48] Rabeharitsara Andry Tahina – Rabearimihaja Phandry Nomena Ndjiva “Betacarotenes Dosage by Hydrofluoric Acid Solution and Validation of This New Process by SPC” - American Journal of Applied Chemistry in Vol. 4, Issue Number 3, June 2016 - Chemical Process Engineering Department (E.S.P.A), Antananarivo University – Madagascar.
Cite This Article
  • APA Style

    Nambinina Richard Randriana, Ernestine Ravomialisoa, Andry Tahina Rabeharitsara. (2021). Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures. American Journal of Applied Chemistry, 9(3), 53-64. https://doi.org/10.11648/j.ajac.20210903.11

    Copy | Download

    ACS Style

    Nambinina Richard Randriana; Ernestine Ravomialisoa; Andry Tahina Rabeharitsara. Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures. Am. J. Appl. Chem. 2021, 9(3), 53-64. doi: 10.11648/j.ajac.20210903.11

    Copy | Download

    AMA Style

    Nambinina Richard Randriana, Ernestine Ravomialisoa, Andry Tahina Rabeharitsara. Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures. Am J Appl Chem. 2021;9(3):53-64. doi: 10.11648/j.ajac.20210903.11

    Copy | Download

  • @article{10.11648/j.ajac.20210903.11,
      author = {Nambinina Richard Randriana and Ernestine Ravomialisoa and Andry Tahina Rabeharitsara},
      title = {Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures},
      journal = {American Journal of Applied Chemistry},
      volume = {9},
      number = {3},
      pages = {53-64},
      doi = {10.11648/j.ajac.20210903.11},
      url = {https://doi.org/10.11648/j.ajac.20210903.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20210903.11},
      abstract = {A washing-disinfectant product was synthesized with citric acid which was a readily available raw material on the chemical markets. Following-up the established procedure to use this product reflected its efficiency to eliminate, to remove, to kill and to decrease significantly the binary fission of various germs and micro-organisms not only on raw materials but also on materials and containers that could cause consumers illness. Indeed, the quantifications of these micro-organisms on a product “spicy product energy booster (speb)” – “produit épicé boosteur d’énergie (pebe)” at the “Institut Pasteur de Madagascar” whose analyzes were accredited by COFRAC © reflected that firstly there were any Salmonella spp. and any Listeria monocytogenes; secondly its quantities of Bacillus cereus, Escherichia coli and Staphylococcus aureus respected the European standardization for food products; thirdly this “speb-pebe” product decreased significantly the binary fission speed of these previous micro-organisms in order 4.7 to 80 times less speed than its speeds deduced by a reference and/or by its generation time; and fourth the product “speb-pebe” best-before dates during a conservation at 303.15 (°K), natural ambient temperature, were calculated from each micro-organism’s binary fission speed. Then, two procedures titrations consisting in titration of the “speb-pebe” - spicy product energy booster’s equivalent-citric acid molecules in each level of its packaging using NaOH-0.0557N and in titration of the “speb-pebe” - spicy product energy booster’s alkenes-C= organic functions (on surface and on structure-texture) in each level of its packaging using HF-0.0026N were established and allowed to appreciate the “speb-pebe” quality in each level of its packaging and also to control its packaging-quality. It was noticed that the concentration of alkenes-C= organic functions on structure and on texture per grams on the Level-high-packaging were 5.9 times less than those on Level-medium-packaging 1.23E-1 [mol×l-1×g-1] and on Level-low-packaging 1.16E-1 [mol×l-1×g-1] confirming in return the highest value of the concentration of alkenes-C= organic functions on surface per grams of “speb-pebe” observed at Level-high-packaging sample (4.805E-3 [mol×l-1×g-1]) compared with Level-middle-packaging sample (1.72E-3 [mol×l-1×g-1]) and with Level-low-packaging sample (2.19E-3 [mol×l-1×g-1]). Another results, the dispersion of the equivalent-citric acids molecules on total alkenes-C= organic functions noted dispersion (Ac⁄C=) or dispersion ([Ac]⁄[C=]) for the Level-high-packaging sample, the Level-middle-packaging sample and the Level-low-packaging sample were respectively 0.2813, 0.0250 and 0.0714 and confirmed the useful role of citric acids molecules quantities and their protons H+ capacities and activities to catalyze some chemistry reactions and responsible of this “speb-pebe” - spicy product energy booster’s acidity-pH=2.62, flavors and tastes.},
     year = {2021}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Washing-Disinfectant Product Synthesis Tested During the Production of the “Speb-Pebe” - Spicy Product Energy Booster Characterized by Established Titration Procedures
    AU  - Nambinina Richard Randriana
    AU  - Ernestine Ravomialisoa
    AU  - Andry Tahina Rabeharitsara
    Y1  - 2021/06/21
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajac.20210903.11
    DO  - 10.11648/j.ajac.20210903.11
    T2  - American Journal of Applied Chemistry
    JF  - American Journal of Applied Chemistry
    JO  - American Journal of Applied Chemistry
    SP  - 53
    EP  - 64
    PB  - Science Publishing Group
    SN  - 2330-8745
    UR  - https://doi.org/10.11648/j.ajac.20210903.11
    AB  - A washing-disinfectant product was synthesized with citric acid which was a readily available raw material on the chemical markets. Following-up the established procedure to use this product reflected its efficiency to eliminate, to remove, to kill and to decrease significantly the binary fission of various germs and micro-organisms not only on raw materials but also on materials and containers that could cause consumers illness. Indeed, the quantifications of these micro-organisms on a product “spicy product energy booster (speb)” – “produit épicé boosteur d’énergie (pebe)” at the “Institut Pasteur de Madagascar” whose analyzes were accredited by COFRAC © reflected that firstly there were any Salmonella spp. and any Listeria monocytogenes; secondly its quantities of Bacillus cereus, Escherichia coli and Staphylococcus aureus respected the European standardization for food products; thirdly this “speb-pebe” product decreased significantly the binary fission speed of these previous micro-organisms in order 4.7 to 80 times less speed than its speeds deduced by a reference and/or by its generation time; and fourth the product “speb-pebe” best-before dates during a conservation at 303.15 (°K), natural ambient temperature, were calculated from each micro-organism’s binary fission speed. Then, two procedures titrations consisting in titration of the “speb-pebe” - spicy product energy booster’s equivalent-citric acid molecules in each level of its packaging using NaOH-0.0557N and in titration of the “speb-pebe” - spicy product energy booster’s alkenes-C= organic functions (on surface and on structure-texture) in each level of its packaging using HF-0.0026N were established and allowed to appreciate the “speb-pebe” quality in each level of its packaging and also to control its packaging-quality. It was noticed that the concentration of alkenes-C= organic functions on structure and on texture per grams on the Level-high-packaging were 5.9 times less than those on Level-medium-packaging 1.23E-1 [mol×l-1×g-1] and on Level-low-packaging 1.16E-1 [mol×l-1×g-1] confirming in return the highest value of the concentration of alkenes-C= organic functions on surface per grams of “speb-pebe” observed at Level-high-packaging sample (4.805E-3 [mol×l-1×g-1]) compared with Level-middle-packaging sample (1.72E-3 [mol×l-1×g-1]) and with Level-low-packaging sample (2.19E-3 [mol×l-1×g-1]). Another results, the dispersion of the equivalent-citric acids molecules on total alkenes-C= organic functions noted dispersion (Ac⁄C=) or dispersion ([Ac]⁄[C=]) for the Level-high-packaging sample, the Level-middle-packaging sample and the Level-low-packaging sample were respectively 0.2813, 0.0250 and 0.0714 and confirmed the useful role of citric acids molecules quantities and their protons H+ capacities and activities to catalyze some chemistry reactions and responsible of this “speb-pebe” - spicy product energy booster’s acidity-pH=2.62, flavors and tastes.
    VL  - 9
    IS  - 3
    ER  - 

    Copy | Download

Author Information
  • Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar

  • Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar

  • Chemical Process Engineering Department (E. S. P. A), Antananarivo University, Antananarivo, Madagascar

  • Sections