Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism.
| Published in | American Journal of Chemical Engineering (Volume 10, Issue 2) |
| DOI | 10.11648/j.ajche.20221002.11 |
| Page(s) | 18-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Mb/DDAB Films, Glassy Carbon (GC) Electrode, Electro-catalysis, EC’ Catalytic Mechanism
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APA Style
Abdelatty Mohamed Radalla. (2022). Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. American Journal of Chemical Engineering, 10(2), 18-22. https://doi.org/10.11648/j.ajche.20221002.11
ACS Style
Abdelatty Mohamed Radalla. Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. Am. J. Chem. Eng. 2022, 10(2), 18-22. doi: 10.11648/j.ajche.20221002.11
AMA Style
Abdelatty Mohamed Radalla. Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode. Am J Chem Eng. 2022;10(2):18-22. doi: 10.11648/j.ajche.20221002.11
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Download@article{10.11648/j.ajche.20221002.11,
author = {Abdelatty Mohamed Radalla},
title = {Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode},
journal = {American Journal of Chemical Engineering},
volume = {10},
number = {2},
pages = {18-22},
doi = {10.11648/j.ajche.20221002.11},
url = {https://doi.org/10.11648/j.ajche.20221002.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20221002.11},
abstract = {Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism.},
year = {2022}
}
TY - JOUR T1 - Coulometric and Chronoamperometric Studies on Bisulfite Reduction at a Surfactant/Myoglobin Film on Glassy Carbon Electrode AU - Abdelatty Mohamed Radalla Y1 - 2022/03/23 PY - 2022 N1 - https://doi.org/10.11648/j.ajche.20221002.11 DO - 10.11648/j.ajche.20221002.11 T2 - American Journal of Chemical Engineering JF - American Journal of Chemical Engineering JO - American Journal of Chemical Engineering SP - 18 EP - 22 PB - Science Publishing Group SN - 2330-8613 UR - https://doi.org/10.11648/j.ajche.20221002.11 AB - Background. The redox-active protein, myoglobin (Mb), exchanges electrons very slowly with bare electrodes. Just like on a highly oriented pyrolytic graphite (HOPG) bare electrode, electron transfer between myoglobin (Mb) and a bare glassy carbon (GC) electrode was not observed. The myoglobin (Mb) in di-dodecyl dimethyl ammonium bromide (DDAB) film immobilized on glassy carbon electrode surface had a good charge transport, allowing Mb to be used as a redox catalyst for multi-electron transfer reactions. Objective. We report here the myoglobin on a glassy carbon (GC) electrode as a catalyst for the multi-electron reduction of bisulfite in aqueous buffered solutions. Methods. Coulometry and chronoamperometry are used as tools to probe the Mb/DDAB film on GC electrode as an effective electro-catalyst for the multi-electron reduction of bisulfite. Results. Variation in current with time of bisulfite reduction followed first-order reaction kinetics. The heterogeneous electron transfer rate constant of the film and catalytic rate constant for the reduction of bisulfite were determined. Conclusion. The study confirmed that bisulfite was the reactive species and the catalytic reduction reaction at Mb/DDAB film followed the EC’ catalytic mechanism. VL - 10 IS - 2 ER -
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