| Peer-Reviewed

Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent

Received: 23 January 2023    Accepted: 20 February 2023    Published: 4 March 2023
Views:       Downloads:
Abstract

Many studies have investigated the technology of anaerobic digestion for waste treatment and its benefits. However, most of those studies have reported on solid waste. So, there are few articles on the anaerobic digestion of effluent, especially anaerobic digestion of slaughterhouse effluent and its bioenergy potential. The purpose of this study is to evaluate the bioenergy potential in slaughterhouse wastewater treatment. Then, anaerobic digestion (AD) was used in this study to assess the bio-energy potential and kinetics of biogas production during processing. The slaughterhouse wastewater collected was characterized before the experiments using french standard method “AFNOR”. pH, Temperature, turbidity, dissolved oxygen (O2dis.), oxidation-reduction potential (ORP), Conductivity, Chemical Oxygen Demand (COD), biochemical oxygen demand in five days (BOD5), total Kjeldahl nitrogen (TKN) and total phosphorus (Ptot) were analyzed and the ratio BOD5/COD was calculated to evaluate the biodegradability of the biomass. Laboratory-scale anaerobic batch digesters consisting of a 1 L plastic container were used in all the experiments and the biogas produced in the digesters was measured daily by the water displacement method. The wastewater produced by slaughterhouses is biodegradable with a ratio between biological oxygen demand and chemical oxygen demand (BOD5/COD) > 0.5. An effective AD design shows that over 90% of organic matter was removed when inoculation and the carbon/nitrogen (C/N) ratio were adjusted. The cumulative volume of biogas increased from 415 mL to 2,150 mL as the substrate/inoculum (S/I) ratio has decreased from 2.028 to 0.337 and increased from 1,140 mL to 5,250 mL as the C/N ratio increased from 6 to 22. The biogas produced has a high calorific value, as the methane content is 74%. Among the three kinetic models used to describe biogas production, a modified Gompertz model was found to be the best with R2 between 0.983 and 0.993. This study points out energy potential of slaughterhouse wastewater and its benefit as it is managed efficiently.

Published in American Journal of Chemical Engineering (Volume 11, Issue 1)
DOI 10.11648/j.ajche.20231101.12
Page(s) 20-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Slaughterhouse Wastewater, Anaerobic Digestion, Bioenergy, Biogas Production, Kinetic of Biogas Generation, Inoculum

References
[1] C. Wee and J.-J. Su (2019) Biofuel Produced from Solid-State Anaerobic Digestion of Dairy Cattle Manure in Coordination with Black Soldier Fly Larvae Decomposition, Energies, vol. 12, no. 5, p. 911, https://doi.org/10.3390/en12050911.
[2] S. Tong, S. Wang, Y. Zhao, C. Feng, B. Xu, and M. Zhu (2019) Enhanced alure-type biological system (E-ATBS) for carbon, nitrogen and phosphorus removal from slaughterhouse wastewater: A case study, Bioresource Technology, vol. 274, pp. 244–251, https://doi.org/10.1016/j.biortech.2018.11.094.
[3] S. Tong, Y. Zhao, M. Zhu, J. Wei, S. Zhang, S. Li, S. Sun (2019) Effect of the supernatant reflux position and ratio on the nitrogen removal performance of anaerobic-aerobic slaughterhouse wastewater treatment process, Environmental Engineering Research, vol. 25, no. 3, pp. 309–315, https://doi.org/10.4491/eer.2019.091.
[4] B. S. Zeb, Q. Mahmood, and A. Pervez (2013) Characteristics and Performance of Anaerobic Wastewater Treatment (A Review), J. Chem.Soc.Pak, vol. 35, no. n°1, pp. 217–232.
[5] E. Duarte, R. Fragoso, N. Smozinski, and J. Tavares (2021) Enhancing Bioenergy Recovery from Agro-food Biowastes as a Strategy to Promote Circular Bioeconomy’, J. sustain. dev. energy water environ. syst., 9 (1), 1080320, https://doi.org/10.13044/j.sdewes.d8.0320.
[6] F. Raposo, C. J. Banks, I. Siegert, S. Heaven, and R. Borja (2006) Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests, Process Biochemistry, vol. 41, no. 6, pp. 1444–1450, https://doi.org/10.1016/j.procbio.2006.01.012.
[7] Tcha-Thom Maglwa (2019) Recherche d’une filière durable pour la méthanisation des déchets de fruits et d’abattoirs du Togo: Evaluation du potentiel agronomique des digestats sur les sols de la région de la Kara. Thèse de doctorat, Université de Lomé et Université de Limoges.
[8] P. Aubry and B.-A. Gaüzère (2021) Les maladies liées à l’eau’. Centre René Labusquière, Institut de Médecine Tropicale, Université de Bordeaux.
[9] P. D. Haras (2017) Analyse et traitement des microorganismes du milieu hydrique, Escherichia coli, p. 28.
[10] S. Milin, Christophe Le Jallé, and Colette Génevaux (2016) Services d’eau et d’assainissement face au changement climatique’. Programme Solidarité-Eau (pS-Eau).
[11] C. A. Sevillano, A. A. Pesantes, E. Peña Carpio, E. J. Martínez, and X. Gómez (2021) Anaerobic Digestion for Producing Renewable Energy—The Evolution of This Technology in a New Uncertain Scenario, Entropy, vol. 23, no. 2, p. 145, https://doi.org/10.3390/e23020145.
[12] L. Moody, R. Burns, W. Wu-Haan, and R. Spajiš (2009) Use of Biochemical Methane Potential (BMP) Assays for Predicting and Enhancing Anaerobic Digester Performance’, Agricultural Engineering, pp. 930–934.
[13] T. Losak, J. Hlusek, A. Zatloukalova, L. Musilova, M. Vitezova, P. Skarpa, T. Zlamalova, J. Fryc, T. Vitez, J. Marecek, A. Martensson, (2014) Digestate from Biogas Plants is an Attractive Alternative to Mineral Fertilisation of Kohlrabi, J. sustain. dev. energy water environ. syst., vol. 2, no. 4, pp. 309–318, https://doi.org/10.13044/j.sdewes.2014.02.0025.
[14] J. Baeyens, L. Appels, L. Peng, and R. Dewil (2016) The production of bio-energy by microbial (biogas through anaerobic digestion) or thermal (pyrolysis) processes, Renewable Energy, vol. 96, p. 1055, https://doi.org/10.1016/j.renene.2016.06.012.
[15] J. Filer, H. H. Ding, and S. Chang (2019) Biochemical Methane Potential (BMP) Assay Method for Anaerobic Digestion Research, Water, vol. 11, no. 5, p. 921, https://doi.org/10.3390/w11050921.
[16] L. Appels, J. Baeyens, J. Degrève, and R. Dewil (2008) Principles and potential of the anaerobic digestion of waste-activated sludge’, Progress in Energy and Combustion Science, vol. 34, no. 6, pp. 755–781, https://doi.org/10.1016/j.pecs.2008.06.002.
[17] I. M. Ramatsa, E. T. Akinlabi, D. M. Madyira, and R. Huberts (2014) Design of the Bio-digester for Biogas Production: A Review, p. 4.
[18] S. K. Pramanik, F. B. Suja, M. Porhemmat, and B. K. Pramanik (2019) Performance and Kinetic Model of a Single-Stage Anaerobic Digestion System Operated at Different Successive Operating Stages for the Treatment of Food Waste, Processes, vol. 7, no. 9, p. 600, https://doi.org/10.3390/pr7090600.
[19] E. Membere and P. Sallis (2018) Effect of temperature on kinetics of biogas production from macroalgae, Bioresource Technology, vol. 263, pp. 410–417, https://doi.org/10.1016/j.biortech.2018.05.023.
[20] B. Deepanraj, V. Sivasubramanian, and S. Jayaraj (2015) Kinetic study on the effect of temperature on biogas production using a lab scale batch reactor, Ecotoxicology and Environmental Safety, vol. 121, pp. 100–104, https://doi.org/10.1016/j.ecoenv.2015.04.051.
[21] M. Y. Nurliyana, P. S. H’ng, H. Rasmina, M. S. Umi Kalsom, K. L. Chin, S. H. Lee, W. C. Lum, G. D. Khoo (2015) Effect of C/N ratio in methane productivity and biodegradability during facultative co-digestion of palm oil mill effluent and empty fruit bunch, Industrial Crops and Products, vol. 76, pp. 409–415, https://doi.org/10.1016/j.indcrop.2015.04.047.
[22] H. Caillet, E. Lebon, E. Akinlabi, D. Madyira, and L. Adelard (2019) Influence of inoculum to substrate ratio on methane production in Biochemical Methane Potential (BMP) tests of sugarcane distillery waste water, Procedia Manufacturing, vol. 35, pp. 259–264, https://doi.org/10.1016/j.promfg.2019.05.037.
[23] B. Hülsemann, L. Zhou, W. Merkle, J. Hassa, J. Müller, and H. Oechsner (2020) Biomethane Potential Test: Influence of Inoculum and the Digestion System, Applied Sciences, vol. 10, no. 7, p. 2589, https://doi.org/10.3390/app10072589.
[24] H. Dhar, P. Kumar, S. Kumar, S. Mukherjee, and A. N. Vaidya (2016) Effect of organic loading rate during anaerobic digestion of municipal solid waste, Bioresource Technology, vol. 217, pp. 56–61, https://doi.org/10.1016/j.biortech.2015.12.004.
[25] A. Babaei and J. Shayegan (2019) Effects of temperature and mixing modes on the performance of municipal solid waste anaerobic slurry digester, J Environ Health Sci Engineer, vol. 17, no. 2, pp. 1077–1084, https://doi.org/10.1007/s40201-019-00422-6.
[26] Association Française de Normalisation (AFNOR) (, 1986) EAUX METHODES D’ESSAI. Paris.
[27] Y.-C. Chou and J.-J. Su (2019) Biogas Production by Anaerobic Co-Digestion of Dairy Wastewater with the Crude Glycerol from Slaughterhouse Sludge Cake Transesterification, Animals, vol. 9, no. 9, p. 618, https://doi.org/10.3390/ani9090618.
[28] W. Li, H. Khalid, F. R. Amin, H. Zhang, Z. Dai, C. Chen, G. Liu (2020) Biomethane production characteristics, kinetic analysis, and energy potential of different paper wastes in anaerobic digestion, Renewable Energy, vol. 157, pp. 1081–1088, https://doi.org/10.1016/j.renene.2020.04.035.
[29] X. Wang, X. Lu, F. Li, and G. Yang (2014) Effects of Temperature and Carbon-Nitrogen (C/N) Ratio on the Performance of Anaerobic Co-Digestion of Dairy Manure, Chicken Manure and Rice Straw: Focusing on Ammonia Inhibition, PLoS ONE, vol. 9, no. 5, p. e97265, https://doi.org/10.1371/journal.pone.0097265.
[30] A. Marcos, A. Al-Kassir, A. A. Mohamad, F. Cuadros, and F. López-Rodríguez (2010) Combustible gas production (methane) and biodegradation of solid and liquid mixtures of meat industry wastes, Applied Energy, vol. 87, no. 5, pp. 1729–1735, https://doi.org/10.1016/j.apenergy.2009.09.037.
[31] S. Zerrouki, R. Rihani, F. Bentahar, and K. Belkacemi (2015) Anaerobic digestion of wastewater from the fruit juice industry: experiments and modeling, Water Science & Technology, vol. 72, no. 1, p. 123, https://doi.org/10.2166/wst.2015.193.
[32] M. Ebrahimi-Nik, A. Heidari, S. Ramezani Azghandi, F. Asadi Mohammadi, and H. Younesi (2018) Drinking water treatment sludge as an effective additive for biogas production from food waste; kinetic evaluation and biomethane potential test, Bioresource Technology, vol. 260, pp. 421–426, https://doi.org/10.1016/j.biortech.2018.03.112.
[33] C. F. Bustillo-Lecompte and M. Mehrvar (2015) Slaughterhouse wastewater characteristics, treatment, and management in the meat processing industry: A review on trends and advances, Journal of Environmental Management, vol. 161, pp. 287–302, https://doi.org/10.1016/j.jenvman.2015.07.008.
[34] A. A. Lawal, A. U. Dzivama, and M. K. Wasinda (2016) Effect of inoculum to substrate ratio on biogas production of sheep paunch manure, Res. Agr. Eng., vol. 62, no. No. 1, pp. 8–14, https://doi.org/10.17221/30/2014-RAE.
[35] I. N. Widiasa and S. Johari (2010) The Kinetic of Biogas Production Rate from Cattle Manure in Batch Mode’, p. 6.
[36] H. I. Owamah et al. (2021) Influence of inoculum/substrate ratio on biogas yield and kinetics from the anaerobic co-digestion of food waste and maize husk, Environmental Nanotechnology, Monitoring & Management, vol. 16, p. 100558, https://doi.org/10.1016/j.enmm.2021.100558.
[37] M. N. Chollom, S. Rathilal, F. M. Swalaha, B. F. Bakare, and E. K. Tetteh (2019) ANAEROBIC TREATMENT OF SLAUGHTERHOUSE WASTEWATER: EVALUATING OPERATING CONDITIONS, Valencia, Spain, pp. 251–262. https://doi.org/10.2495/WS190221.
[38] T. O. Emmanuel, K. Bawo, and I. E. Lawrence (2016) Evaluation of bacterial profile and biodegradation potential of abattoir wastewater’, African Journal of Environmental Science and Technology, vol. 10, no. 2, pp. 50–57, https://doi.org/10.5897/AJEST2015.1945.
[39] C. Zhang, H. Su, J. Baeyens, and T. Tan (2014) Reviewing the anaerobic digestion of food waste for biogas production, Renewable and Sustainable Energy Reviews, vol. 38, pp. 383–392, https://doi.org/10.1016/j.rser.2014.05.038.
[40] F. Raposo, V. Fernández-Cegri, M. A. De la Rubia, Béline, F.; Cavinato, C.; Demirer, G.; Fernández, B.; Fernández-Polanco, M.; Frigon, J.-C. (2011) Biochemical methane potential (BMP) of solid organic substrates: evaluation of anaerobic biodegradability using data from an international interlaboratory study, Journal of Chemical Technology & Biotechnology, vol. 86, no. 8, pp. 1088–1098, https://doi.org/10.1002/jctb.2622.
[41] R. M. Panizio, L. F. do C. Calado, G. Lourinho, P. S. D. de Brito, and J. B. Mees (2019) Potential of Biogas Production in Anaerobic Co-digestion of Opuntia ficus-indica and Slaughterhouse Wastes’, Waste Biomass Valor, https://doi.org/10.1007/s12649-019-00835-2.
[42] M. Shahbaz, M. Ammar; K.R. Korai, N. Ahmad, A. Ali (2020) Impact of C/N ratios and organic loading rates of paper, cardboard and tissue wastes in batch and CSTR anaerobic digestion with food waste on their biogas production and digester stability, SN Appl. Sci., vol. 2, no. 8, p. 1436, https://doi.org/10.1007/s42452-020-03232-w.
[43] A. A. Akindele and M. Sartaj (2018) The toxicity effects of ammonia on anaerobic digestion of organic fraction of municipal solid waste, Waste Management, vol. 71, pp. 757–766, https://doi.org/10.1016/j.wasman.2017.07.026.
[44] I. Zawieja, R. Włodarczyk, and M. Kowalczyk, ‘Biogas Generation from Sonicated Excess Sludge’, Water, vol. 11, no. 10, p. 2127, Oct. 2019, https://doi.org/10.3390/w11102127.
[45] T. Forster-Carneiro, M. Pérez, and L. I. Romero (2008) Thermophilic anaerobic digestion of source-sorted organic fraction of municipal solid waste, Bioresource Technology, vol. 99, no. 15, pp. 6763–6770, https://doi.org/10.1016/j.biortech.2008.01.052.
[46] W. Liu, C. Liu, P. Gogoi, and Y. Deng (2020) Overview of Biomass Conversion to Electricity and Hydrogen and Recent Developments in Low-Temperature Electrochemical Approaches, Engineering, vol. 6, no. 12, pp. 1351–1363, https://doi.org/10.1016/j.eng.2020.02.021.
[47] C. Mao, X. Wang, J. Xi, Y. Feng, and G. Ren (2017) Linkage of kinetic parameters with process parameters and operational conditions during anaerobic digestion, Energy, vol. 135, pp. 352–360, https://doi.org/10.1016/j.energy.2017.06.050.
[48] C. Mao, T. Zhang, X. Wang, Y. Feng, G. Ren, and G. Yang (2017) Process performance and methane production optimizing of anaerobic co-digestion of swine manure and corn straw, Sci Rep, vol. 7, no. 1, p. 9379, https://doi.org/10.1038/s41598-017-09977-6.
[49] L. Noynoo, H. Thongpan, S. Jijai, N. Ismail, and N. Rakmak (2020) Anaerobic digestion of synthetic wastewater (acetate): effect of COD and C/N ratio in batch mode’, p. 11.
Cite This Article
  • APA Style

    Dègninou Houndedjihou, Tomkouani Kodom, Ibrahim Tchakala, Moctar Limam Bawa, Gbandi Djaneye-Boundjou. (2023). Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent. American Journal of Chemical Engineering, 11(1), 20-32. https://doi.org/10.11648/j.ajche.20231101.12

    Copy | Download

    ACS Style

    Dègninou Houndedjihou; Tomkouani Kodom; Ibrahim Tchakala; Moctar Limam Bawa; Gbandi Djaneye-Boundjou. Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent. Am. J. Chem. Eng. 2023, 11(1), 20-32. doi: 10.11648/j.ajche.20231101.12

    Copy | Download

    AMA Style

    Dègninou Houndedjihou, Tomkouani Kodom, Ibrahim Tchakala, Moctar Limam Bawa, Gbandi Djaneye-Boundjou. Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent. Am J Chem Eng. 2023;11(1):20-32. doi: 10.11648/j.ajche.20231101.12

    Copy | Download

  • @article{10.11648/j.ajche.20231101.12,
      author = {Dègninou Houndedjihou and Tomkouani Kodom and Ibrahim Tchakala and Moctar Limam Bawa and Gbandi Djaneye-Boundjou},
      title = {Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent},
      journal = {American Journal of Chemical Engineering},
      volume = {11},
      number = {1},
      pages = {20-32},
      doi = {10.11648/j.ajche.20231101.12},
      url = {https://doi.org/10.11648/j.ajche.20231101.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20231101.12},
      abstract = {Many studies have investigated the technology of anaerobic digestion for waste treatment and its benefits. However, most of those studies have reported on solid waste. So, there are few articles on the anaerobic digestion of effluent, especially anaerobic digestion of slaughterhouse effluent and its bioenergy potential. The purpose of this study is to evaluate the bioenergy potential in slaughterhouse wastewater treatment. Then, anaerobic digestion (AD) was used in this study to assess the bio-energy potential and kinetics of biogas production during processing. The slaughterhouse wastewater collected was characterized before the experiments using french standard method “AFNOR”. pH, Temperature, turbidity, dissolved oxygen (O2dis.), oxidation-reduction potential (ORP), Conductivity, Chemical Oxygen Demand (COD), biochemical oxygen demand in five days (BOD5), total Kjeldahl nitrogen (TKN) and total phosphorus (Ptot) were analyzed and the ratio BOD5/COD was calculated to evaluate the biodegradability of the biomass. Laboratory-scale anaerobic batch digesters consisting of a 1 L plastic container were used in all the experiments and the biogas produced in the digesters was measured daily by the water displacement method. The wastewater produced by slaughterhouses is biodegradable with a ratio between biological oxygen demand and chemical oxygen demand (BOD5/COD) > 0.5. An effective AD design shows that over 90% of organic matter was removed when inoculation and the carbon/nitrogen (C/N) ratio were adjusted. The cumulative volume of biogas increased from 415 mL to 2,150 mL as the substrate/inoculum (S/I) ratio has decreased from 2.028 to 0.337 and increased from 1,140 mL to 5,250 mL as the C/N ratio increased from 6 to 22. The biogas produced has a high calorific value, as the methane content is 74%. Among the three kinetic models used to describe biogas production, a modified Gompertz model was found to be the best with R2 between 0.983 and 0.993. This study points out energy potential of slaughterhouse wastewater and its benefit as it is managed efficiently.},
     year = {2023}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent
    AU  - Dègninou Houndedjihou
    AU  - Tomkouani Kodom
    AU  - Ibrahim Tchakala
    AU  - Moctar Limam Bawa
    AU  - Gbandi Djaneye-Boundjou
    Y1  - 2023/03/04
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ajche.20231101.12
    DO  - 10.11648/j.ajche.20231101.12
    T2  - American Journal of Chemical Engineering
    JF  - American Journal of Chemical Engineering
    JO  - American Journal of Chemical Engineering
    SP  - 20
    EP  - 32
    PB  - Science Publishing Group
    SN  - 2330-8613
    UR  - https://doi.org/10.11648/j.ajche.20231101.12
    AB  - Many studies have investigated the technology of anaerobic digestion for waste treatment and its benefits. However, most of those studies have reported on solid waste. So, there are few articles on the anaerobic digestion of effluent, especially anaerobic digestion of slaughterhouse effluent and its bioenergy potential. The purpose of this study is to evaluate the bioenergy potential in slaughterhouse wastewater treatment. Then, anaerobic digestion (AD) was used in this study to assess the bio-energy potential and kinetics of biogas production during processing. The slaughterhouse wastewater collected was characterized before the experiments using french standard method “AFNOR”. pH, Temperature, turbidity, dissolved oxygen (O2dis.), oxidation-reduction potential (ORP), Conductivity, Chemical Oxygen Demand (COD), biochemical oxygen demand in five days (BOD5), total Kjeldahl nitrogen (TKN) and total phosphorus (Ptot) were analyzed and the ratio BOD5/COD was calculated to evaluate the biodegradability of the biomass. Laboratory-scale anaerobic batch digesters consisting of a 1 L plastic container were used in all the experiments and the biogas produced in the digesters was measured daily by the water displacement method. The wastewater produced by slaughterhouses is biodegradable with a ratio between biological oxygen demand and chemical oxygen demand (BOD5/COD) > 0.5. An effective AD design shows that over 90% of organic matter was removed when inoculation and the carbon/nitrogen (C/N) ratio were adjusted. The cumulative volume of biogas increased from 415 mL to 2,150 mL as the substrate/inoculum (S/I) ratio has decreased from 2.028 to 0.337 and increased from 1,140 mL to 5,250 mL as the C/N ratio increased from 6 to 22. The biogas produced has a high calorific value, as the methane content is 74%. Among the three kinetic models used to describe biogas production, a modified Gompertz model was found to be the best with R2 between 0.983 and 0.993. This study points out energy potential of slaughterhouse wastewater and its benefit as it is managed efficiently.
    VL  - 11
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Chemistry Department, Faculty of Sciences, University of Lome (UL), Lome, Togo

  • Chemistry Department, Faculty of Sciences, University of Lome (UL), Lome, Togo

  • Chemistry Department, Faculty of Sciences, University of Lome (UL), Lome, Togo

  • Chemistry Department, Faculty of Sciences, University of Lome (UL), Lome, Togo

  • Chemistry Department, Faculty of Sciences, University of Lome (UL), Lome, Togo

  • Sections