Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor

Teresa Matoso Manguangua Victor; Jarka Glassey; Kristie Kamps; Alan Claude Ward

doi:doi:10.11648/j.ajche.20231104.11

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Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor

Teresa Matoso Manguangua Victor^*, Jarka Glassey, Kristie Kamps, Alan Claude Ward

Published in American Journal of Chemical Engineering (Volume 11, Issue 4)

Received: 30 September 2023 Accepted: 24 October 2023 Published: 9 November 2023

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Abstract

In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml^-1h^-1) and actinorhodin (26.08 μmol ml^-1h^-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.

Published in	American Journal of Chemical Engineering (Volume 11, Issue 4)
DOI	10.11648/j.ajche.20231104.11
Page(s)	64-74
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

PolyHIPE Polymer (PHP), Solid State Fermentation (SSF), Streptomyces coelicolor A3(2) Prodigiosin, Actinorhodin, Antimicrobial Screening

References

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APA Style

Victor, T. M. M., Glassey, J., Kamps, K., Ward, A. C. (2023). Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. American Journal of Chemical Engineering, 11(4), 64-74. https://doi.org/10.11648/j.ajche.20231104.11

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ACS Style

Victor, T. M. M.; Glassey, J.; Kamps, K.; Ward, A. C. Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. Am. J. Chem. Eng. 2023, 11(4), 64-74. doi: 10.11648/j.ajche.20231104.11

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AMA Style

Victor TMM, Glassey J, Kamps K, Ward AC. Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. Am J Chem Eng. 2023;11(4):64-74. doi: 10.11648/j.ajche.20231104.11

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@article{10.11648/j.ajche.20231104.11,
  author = {Teresa Matoso Manguangua Victor and Jarka Glassey and Kristie Kamps and Alan Claude Ward},
  title = {Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor},
  journal = {American Journal of Chemical Engineering},
  volume = {11},
  number = {4},
  pages = {64-74},
  doi = {10.11648/j.ajche.20231104.11},
  url = {https://doi.org/10.11648/j.ajche.20231104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20231104.11},
  abstract = {In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml-1h-1) and actinorhodin (26.08 μmol ml-1h-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.
},
 year = {2023}
}

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TY  - JOUR
T1  - Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor
AU  - Teresa Matoso Manguangua Victor
AU  - Jarka Glassey
AU  - Kristie Kamps
AU  - Alan Claude Ward
Y1  - 2023/11/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajche.20231104.11
DO  - 10.11648/j.ajche.20231104.11
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 64
EP  - 74
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20231104.11
AB  - In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml-1h-1) and actinorhodin (26.08 μmol ml-1h-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.

VL  - 11
IS  - 4
ER  -

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Author Information

Teresa Matoso Manguangua Victor

Department of Engineering and Technology, Instituto Superior Politécnico de Tecnologías y Ciencias, (ISPTEC), Luanda, Angola; School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle Upon Tyne, United Kingdom

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Jarka Glassey

School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle Upon Tyne, United Kingdom
Kristie Kamps

Department of Medicine, University of South Florida, Florida, USA
Alan Claude Ward

School of Biology and Psychology, Newcastle University, Newcastle Upon Tyne, United Kingdom

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APA Style

Victor, T. M. M., Glassey, J., Kamps, K., Ward, A. C. (2023). Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. American Journal of Chemical Engineering, 11(4), 64-74. https://doi.org/10.11648/j.ajche.20231104.11

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ACS Style

Victor, T. M. M.; Glassey, J.; Kamps, K.; Ward, A. C. Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. Am. J. Chem. Eng. 2023, 11(4), 64-74. doi: 10.11648/j.ajche.20231104.11

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AMA Style

Victor TMM, Glassey J, Kamps K, Ward AC. Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor. Am J Chem Eng. 2023;11(4):64-74. doi: 10.11648/j.ajche.20231104.11

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@article{10.11648/j.ajche.20231104.11,
  author = {Teresa Matoso Manguangua Victor and Jarka Glassey and Kristie Kamps and Alan Claude Ward},
  title = {Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor},
  journal = {American Journal of Chemical Engineering},
  volume = {11},
  number = {4},
  pages = {64-74},
  doi = {10.11648/j.ajche.20231104.11},
  url = {https://doi.org/10.11648/j.ajche.20231104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajche.20231104.11},
  abstract = {In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml-1h-1) and actinorhodin (26.08 μmol ml-1h-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.
},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Antibiotic Production Through Solid-State Fermentation Under a Novel Fixed-Bed Micro-Reactor
AU  - Teresa Matoso Manguangua Victor
AU  - Jarka Glassey
AU  - Kristie Kamps
AU  - Alan Claude Ward
Y1  - 2023/11/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ajche.20231104.11
DO  - 10.11648/j.ajche.20231104.11
T2  - American Journal of Chemical Engineering
JF  - American Journal of Chemical Engineering
JO  - American Journal of Chemical Engineering
SP  - 64
EP  - 74
PB  - Science Publishing Group
SN  - 2330-8613
UR  - https://doi.org/10.11648/j.ajche.20231104.11
AB  - In the present study, solid state fermentation (SSF) was evaluated using a novel PolyHIPE Polymer (PHP) matrix. This matrix was developed with an approach to improve the production of antibiotics. For the production of the matrix, a batch reactor was operated with a mixing speed of 300 rpm at differents mixing times (5, 10, 15 min) as a consequence, differents pores sizes was Obtained (55, 39, 19 μm) with a sulfonated/neutralized surface chemistry. After functionalization and purification, the matrix was placed in a fixed-bed micro-reactor, designed specifically for this project; which had 16 fixed beds for the production of antibiotics in the solid state using a model filamentous bacteria Streptomyces coelicolor A3(2). The growth conditions such as the size of the pores of the matrix, were investigated in relation to the growth time (From 0 to 168 hours), and two extracts were produced, Prodigiosin a member of the family of red pigment tripyrrol and Actinorhodin a benzoisochromoquinone dimeric antibiotic that belongs to a class of aromatic polykets. The concentration of the extracted antibiotics and their activities were examined by the disc diffusion method. The two compounds produced were tested against microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, and Penicillium notatum) and the inhibition effects were measured. The results concluded that the highest specific production rate of prodigiosin (3.02 μmol ml-1h-1) and actinorhodin (26.08 μmol ml-1h-1), was achieved within the PHP matrix, with pore sizes of 39 μm and 19 μm in diameter respectively. On the other hand, the assay revealed a larger inhibition halo (diameter in mm) that was observed in the plate inoculated with DSM 10 strains (Bacillus subtilis), inhibited by Prodigiosin extract.

VL  - 11
IS  - 4
ER  -

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