Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

Mandour H. Abdelhai; Hinawi A. M. Hassanin; Xiulan Sun

doi:doi:10.11648/j.bio.20160401.11

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Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria

Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun

Published in American Journal of Bioscience and Bioengineering (Volume 4, Issue 1)

Received: 4 January 2016 Accepted: 13 January 2016 Published: 27 January 2016

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Abstract

Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.

Published in	American Journal of Bioscience and Bioengineering (Volume 4, Issue 1)
DOI	10.11648/j.bio.20160401.11
Page(s)	1-8
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

DNA Extraction, Food Safety, Polymerase Chain Reaction, Pathogenic Bacteria

References

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APA Style

Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun. (2016). Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. American Journal of Bioscience and Bioengineering, 4(1), 1-8. https://doi.org/10.11648/j.bio.20160401.11

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ACS Style

Mandour H. Abdelhai; Hinawi A. M. Hassanin; Xiulan Sun. Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. Am. J. BioSci. Bioeng. 2016, 4(1), 1-8. doi: 10.11648/j.bio.20160401.11

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AMA Style

Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun. Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. Am J BioSci Bioeng. 2016;4(1):1-8. doi: 10.11648/j.bio.20160401.11

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@article{10.11648/j.bio.20160401.11,
  author = {Mandour H. Abdelhai and Hinawi A. M. Hassanin and Xiulan Sun},
  title = {Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {4},
  number = {1},
  pages = {1-8},
  doi = {10.11648/j.bio.20160401.11},
  url = {https://doi.org/10.11648/j.bio.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20160401.11},
  abstract = {Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.},
 year = {2016}
}

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TY  - JOUR
T1  - Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria
AU  - Mandour H. Abdelhai
AU  - Hinawi A. M. Hassanin
AU  - Xiulan Sun
Y1  - 2016/01/27
PY  - 2016
N1  - https://doi.org/10.11648/j.bio.20160401.11
DO  - 10.11648/j.bio.20160401.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 1
EP  - 8
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20160401.11
AB  - Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.
VL  - 4
IS  - 1
ER  -

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Author Information

Mandour H. Abdelhai

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu, China
Hinawi A. M. Hassanin

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Wuxi, Jiangsu, China
Xiulan Sun

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu, China

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Plain Text BibTeX RIS

APA Style

Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun. (2016). Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. American Journal of Bioscience and Bioengineering, 4(1), 1-8. https://doi.org/10.11648/j.bio.20160401.11

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ACS Style

Mandour H. Abdelhai; Hinawi A. M. Hassanin; Xiulan Sun. Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. Am. J. BioSci. Bioeng. 2016, 4(1), 1-8. doi: 10.11648/j.bio.20160401.11

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AMA Style

Mandour H. Abdelhai, Hinawi A. M. Hassanin, Xiulan Sun. Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria. Am J BioSci Bioeng. 2016;4(1):1-8. doi: 10.11648/j.bio.20160401.11

Copy | Download

@article{10.11648/j.bio.20160401.11,
  author = {Mandour H. Abdelhai and Hinawi A. M. Hassanin and Xiulan Sun},
  title = {Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {4},
  number = {1},
  pages = {1-8},
  doi = {10.11648/j.bio.20160401.11},
  url = {https://doi.org/10.11648/j.bio.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20160401.11},
  abstract = {Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.},
 year = {2016}
}

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TY  - JOUR
T1  - Comparative Study of Rapid DNA Extraction Methods of Pathogenic Bacteria
AU  - Mandour H. Abdelhai
AU  - Hinawi A. M. Hassanin
AU  - Xiulan Sun
Y1  - 2016/01/27
PY  - 2016
N1  - https://doi.org/10.11648/j.bio.20160401.11
DO  - 10.11648/j.bio.20160401.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 1
EP  - 8
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20160401.11
AB  - Detection of pathogenic bacteria in food is most important for food safety and quality control, and the critical step it chooses the rapid, sensitive and more economical method to extract DNA to produce high quality and decrease the time-consuming of measuring. Extraction of nucleic acids is the first step in most molecular biology studies and in all recombinant DNA techniques, but the difficult access steps and critical of analysis. Here we report, describe and compare the simple and fast methods of extraction (physical, boiling, phenol/ethanol and commercial kit) methods, from pure culture and then from beef samples. The quantity and quality of extraction methods were confirmed by polymerase chain reaction, agarose gel electrophoresis, and spectrophotometer nanodrop. Results revealed that the efficiently for all three methods were significant compared with the commercial kit, however, in pure culture the boiling method sex tract its more efficient, convenient and cheaper method for template preparation and significant when it compare with other methods while in beef samples experimental results showed that the phenol/ethanol method extract its more significantly.
VL  - 4
IS  - 1
ER  -

Copy | Download