Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2.
| Published in | Cancer Research Journal (Volume 11, Issue 3) |
| DOI | 10.11648/j.crj.20231103.11 |
| Page(s) | 86-90 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Colorectal Cancer, CDH2, miR-218-5p
| [1] | Siegel RL, Miller KD, Sauer AG, Fedewa SA, Butterly LF, Anderson JC, Cercek A, Smith RA & Jemal A. (2020). Colorectal cancer statistics, 2020. CA: a cancer journal for clinicians (3). doi: 10.3322/caac.21601. |
| [2] | Yi Zhi, Jinhong Pan, Wenhao Shen, Peng He, Ji Zheng, Xiaozhou Zhou, Gensheng Lu, Zhiwen Chen & Zhansong Zhou. (2016). Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p. Cell Physiol Biochem (5). doi: 10.1159/000447878. |
| [3] | Zhu K, Ding H, Wang W, Liao ZC, Fu Z, Hong YT, Zhou Y, Zhang CY & Chen X. (2016). Tumor-suppressive miR-218-5p inhibits cancer cell proliferation and migration via EGFR in non-small cell lung cancer. Oncotarget (19). doi: 10.18632/oncotarget.8576. |
| [4] | Zhu L, Tu H, Liang Y &Tang D. (2018). MiR-218 produces anti-tumor effects on cervical cancer cells in vitro. World Journal of Surgical Oncology 16 (1). doi: 10.1186/s12957-018-1506-3. |
| [5] | Wang DZ, Jing SF, Hao SB, Huang XY, Miao QT & Gao JF. (2020). MiR-218 promotes apoptosis of U2OS osteosarcoma cells through targeting BIRC5. European review for medical and pharmacological sciences (16). doi: 10.26355/eurrev_202008_22582. |
| [6] | Pan YY, Wu AH, Xu FF, Chen C, Jiang L & Jin R. (2018). Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer. Oncology Letters (5). doi: 10.3892/ol.2018.8194. |
| [7] | Livak KJ & Schmittgen TD. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods (4). doi: 10.1006/meth.2001.1262. |
| [8] | H Li, Q He, G-M Zhou, W-J Wang, P-P Shi & Z-H Wang. (2023). Potential biomarkers for the prognosis and treatment of HCC immunotherapy. Eur Rev Med Pharmacol Sci (5). doi: 10.26355/eurrev_202303_31569. |
| [9] | Zoi Piperigkou, Kyriaki Tzaferi, George Makrokanis, Konstantina Cheli & Nikos K Karamanos. (2022). The microRNA-cell surface proteoglycan axis in cancer progression. Am J Physiol Cell Physiol (5). doi: 10.1152/ajpcell.00041.2022. |
| [10] | Min Deng, Chao Zeng, Xihong Lu, Xiusheng He, Ruixin Zhang, Qinwei Qiu, Guopei Zheng, Xiaoting Jia, Hao Liu & Zhimin He. (2017). miR-218 suppresses gastric cancer cell cycle progression through the CDK6/Cyclin D1/E2F1 axis in a feedback loop. Cancer Letters (403). doi: 10.1016/j.canlet.2017.06.006. |
| [11] | Yang L, Xu Q, Xie H, Gu G & Jiang J. (2016). Expression of serum miR-218 in hepatocellular carcinoma and its prognostic significance. Clinical and Translational Oncology (8). doi: 10.1007/s12094-015-1447-z. |
| [12] | Peng P, Chen T, Wang Q, Zhang YX, Zheng FF, Huang S, Tang YB, Yang CX, Ding WQ, Ren D, Huang ZW & Guo YQ. (2019). Decreased miR-218-5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer. Oncology Research & Treatment (4). doi: 10.1159/000495473. |
| [13] | Kubelt C, Hattermann K, Sebens S, Mehdorn HM & Held-Feindt J. (2015). Epithelial-to-mesenchymal transition in paired human primary and recurrent glioblastomas. International Journal of Oncology (6). doi: 10.3892/ijo.2015.2944. |
| [14] | Alimperti S & Andreadis ST. (2015). CDH2 and CDH11 act as regulators of stem cell fate decisions. Stem Cell Research (3). doi: 10.1016/j.scr.2015.02.002. |
| [15] | Chen Q, Cai JQ & Jiang CL. (2018). CDH2 expression is of prognostic significance in glioma and predicts the efficacy of temozolomide therapy in patients with glioblastοma. Oncology Letters (5). doi: 10.3892/ol.2018.8227. |
APA Style
Hailong Zhu, Xuyou Zhu, Xiaoli Li, Ran Cui. (2023). Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Research Journal, 11(3), 86-90. https://doi.org/10.11648/j.crj.20231103.11
ACS Style
Hailong Zhu; Xuyou Zhu; Xiaoli Li; Ran Cui. Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Res. J. 2023, 11(3), 86-90. doi: 10.11648/j.crj.20231103.11
AMA Style
Hailong Zhu, Xuyou Zhu, Xiaoli Li, Ran Cui. Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer. Cancer Res J. 2023;11(3):86-90. doi: 10.11648/j.crj.20231103.11
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Download@article{10.11648/j.crj.20231103.11,
author = {Hailong Zhu and Xuyou Zhu and Xiaoli Li and Ran Cui},
title = {Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer},
journal = {Cancer Research Journal},
volume = {11},
number = {3},
pages = {86-90},
doi = {10.11648/j.crj.20231103.11},
url = {https://doi.org/10.11648/j.crj.20231103.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20231103.11},
abstract = {Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2.},
year = {2023}
}
TY - JOUR T1 - Lentivirus-Mediated Overexpression of miR-218-5p Inhibits Metastasis by Targeting CDH2 in Colorectal Cancer AU - Hailong Zhu AU - Xuyou Zhu AU - Xiaoli Li AU - Ran Cui Y1 - 2023/07/06 PY - 2023 N1 - https://doi.org/10.11648/j.crj.20231103.11 DO - 10.11648/j.crj.20231103.11 T2 - Cancer Research Journal JF - Cancer Research Journal JO - Cancer Research Journal SP - 86 EP - 90 PB - Science Publishing Group SN - 2330-8214 UR - https://doi.org/10.11648/j.crj.20231103.11 AB - Introduction: MicroRNA-218 (miR-218) acts as a tumor suppressor in various types of cancer. However, the association between miR-218-5p, the mature body of miR-218, and colorectal cancer (CRC) metastasis remains unclear. Objective: This study aimed to investigate the effect of miR-218-5p on CRC metastasis in vivo and the underlying molecular mechanism. Methods: Quantitative real-time PCR with reverse transcription (RT-qPCR) was employed to determine the expression level of miR-218-5p that trying to investigate if there is a link to clinicopathological characteristics. HCT116 cells were transfected with lentivirus for stably expressing miR-218-5p which was validated, as well as its target gene CDH2, by RT-qPCR and Western blotting. To generate xenograft models, BALB/c nude mice were treated with transfected cells for stably overexpressing miR-218-5p. Finally, the lung metastasis of CRC was observed. Results: The miR-218-5p expression was significantly downregulated in CRC tissues, playing the role of indicator for lymph node metastasis, advanced clinical stage, and poor prognosis. In addition, the expression level of CDH2 was negatively related to miR-218-5p in lentiviral transfected HCT116 cells. At the same time, overexpressed miR-218-5p inhibited CRC cell xenografts metastasis. Conclusion: Data indicates that miR-218-5p suppresses the metastasis of CRC cells, in part by inhibiting CDH2. VL - 11 IS - 3 ER -
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