A variety of microbes can inhabit in extreme conditions. Extreme is a relative term which is viewed compared to what is normal for human beings. These microorganisms are known as oligotrophs. This work aimed to benefit from such organisms isolated from poor soils prevailing in fayoum Governorate. A survey of these organisms (specially) olignitrotrophs and oligocarbotrophs) was made on different soil samples. One hundred and twelve isolates were obtained from eight different soil sites. Ninety eight were obtained from NB/1000 (NB= nutrient broth) and fourteen from NB/10000 dilution. Fixed counts of the different isolates, when inoculated in NB medium with its different dilutions, were almost of constant counts for 30 days incubation at 30°C proved to be facultative not obligate oligotrophs. Eleven isolates produce IAA in amounts averaged 15 µg/ml and the rest were below 15 µg/ml. Five isolates only were capable to produce salyselic acids (S.A.) in appreciable amounts (< 100 µg/ml) and the rest was below 100 µg/ml. fifty four isolates solubilized zink (< 10 mm clear zone), and the rest was below 5 mm halo zone. Nine isolates solubilize phosphate (10mm clear zone) and the rest was below 5mm diameter. Fourty-seven isolates were able to fix nitrogen (olignitrotrophic) in medium deprived completely of N-source. Seventeen isolates were G- and the rest was G+. All isolates were oxidase and catalase positive but cannot ferment lactose. Most isolates were motile (95.5%), spore formers (76.6%) and all were short or long rods. Cellulase enzyme produced by seventeen and chitinase by five isolates only. All isolates examined were facultative oligotrophs as they grew on silica gel NB medium deprived of any source of carbon as well as on complete NB medium.
| Published in | Frontiers in Environmental Microbiology (Volume 2, Issue 2) |
| DOI | 10.11648/j.fem.20160202.11 |
| Page(s) | 6-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Oligotrophs, Oligonitrotrophs, Oligocarbotrophs, Extremophiles, Putative N2-fixers, Biocontrol Agents
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APA Style
Sayed Abdelaziz, Khaled Elbanna, Rabee Elshahawy. (2016). Prevalence and Characterization of Putative Oligotrophic Bacteria in Fayoum Soils, Egypt. Frontiers in Environmental Microbiology, 2(2), 6-11. https://doi.org/10.11648/j.fem.20160202.11
ACS Style
Sayed Abdelaziz; Khaled Elbanna; Rabee Elshahawy. Prevalence and Characterization of Putative Oligotrophic Bacteria in Fayoum Soils, Egypt. Front. Environ. Microbiol. 2016, 2(2), 6-11. doi: 10.11648/j.fem.20160202.11
AMA Style
Sayed Abdelaziz, Khaled Elbanna, Rabee Elshahawy. Prevalence and Characterization of Putative Oligotrophic Bacteria in Fayoum Soils, Egypt. Front Environ Microbiol. 2016;2(2):6-11. doi: 10.11648/j.fem.20160202.11
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author = {Sayed Abdelaziz and Khaled Elbanna and Rabee Elshahawy},
title = {Prevalence and Characterization of Putative Oligotrophic Bacteria in Fayoum Soils, Egypt},
journal = {Frontiers in Environmental Microbiology},
volume = {2},
number = {2},
pages = {6-11},
doi = {10.11648/j.fem.20160202.11},
url = {https://doi.org/10.11648/j.fem.20160202.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20160202.11},
abstract = {A variety of microbes can inhabit in extreme conditions. Extreme is a relative term which is viewed compared to what is normal for human beings. These microorganisms are known as oligotrophs. This work aimed to benefit from such organisms isolated from poor soils prevailing in fayoum Governorate. A survey of these organisms (specially) olignitrotrophs and oligocarbotrophs) was made on different soil samples. One hundred and twelve isolates were obtained from eight different soil sites. Ninety eight were obtained from NB/1000 (NB= nutrient broth) and fourteen from NB/10000 dilution. Fixed counts of the different isolates, when inoculated in NB medium with its different dilutions, were almost of constant counts for 30 days incubation at 30°C proved to be facultative not obligate oligotrophs. Eleven isolates produce IAA in amounts averaged 15 µg/ml and the rest were below 15 µg/ml. Five isolates only were capable to produce salyselic acids (S.A.) in appreciable amounts (< 100 µg/ml) and the rest was below 100 µg/ml. fifty four isolates solubilized zink (< 10 mm clear zone), and the rest was below 5 mm halo zone. Nine isolates solubilize phosphate (10mm clear zone) and the rest was below 5mm diameter. Fourty-seven isolates were able to fix nitrogen (olignitrotrophic) in medium deprived completely of N-source. Seventeen isolates were G- and the rest was G+. All isolates were oxidase and catalase positive but cannot ferment lactose. Most isolates were motile (95.5%), spore formers (76.6%) and all were short or long rods. Cellulase enzyme produced by seventeen and chitinase by five isolates only. All isolates examined were facultative oligotrophs as they grew on silica gel NB medium deprived of any source of carbon as well as on complete NB medium.},
year = {2016}
}
TY - JOUR T1 - Prevalence and Characterization of Putative Oligotrophic Bacteria in Fayoum Soils, Egypt AU - Sayed Abdelaziz AU - Khaled Elbanna AU - Rabee Elshahawy Y1 - 2016/09/18 PY - 2016 N1 - https://doi.org/10.11648/j.fem.20160202.11 DO - 10.11648/j.fem.20160202.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 6 EP - 11 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20160202.11 AB - A variety of microbes can inhabit in extreme conditions. Extreme is a relative term which is viewed compared to what is normal for human beings. These microorganisms are known as oligotrophs. This work aimed to benefit from such organisms isolated from poor soils prevailing in fayoum Governorate. A survey of these organisms (specially) olignitrotrophs and oligocarbotrophs) was made on different soil samples. One hundred and twelve isolates were obtained from eight different soil sites. Ninety eight were obtained from NB/1000 (NB= nutrient broth) and fourteen from NB/10000 dilution. Fixed counts of the different isolates, when inoculated in NB medium with its different dilutions, were almost of constant counts for 30 days incubation at 30°C proved to be facultative not obligate oligotrophs. Eleven isolates produce IAA in amounts averaged 15 µg/ml and the rest were below 15 µg/ml. Five isolates only were capable to produce salyselic acids (S.A.) in appreciable amounts (< 100 µg/ml) and the rest was below 100 µg/ml. fifty four isolates solubilized zink (< 10 mm clear zone), and the rest was below 5 mm halo zone. Nine isolates solubilize phosphate (10mm clear zone) and the rest was below 5mm diameter. Fourty-seven isolates were able to fix nitrogen (olignitrotrophic) in medium deprived completely of N-source. Seventeen isolates were G- and the rest was G+. All isolates were oxidase and catalase positive but cannot ferment lactose. Most isolates were motile (95.5%), spore formers (76.6%) and all were short or long rods. Cellulase enzyme produced by seventeen and chitinase by five isolates only. All isolates examined were facultative oligotrophs as they grew on silica gel NB medium deprived of any source of carbon as well as on complete NB medium. VL - 2 IS - 2 ER -
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