The number of indications for total hip and knee surgery is increasing. The anesthesia and analgesia techniques are very important and effectivity on success of total hip and knee arthroplasty. Multimodal analgesia makes a great contribution to pain treatment after surgery. Periarticular injection of an analgesic solution is a component of multimodal analgesia. However, it has long been unclear whether such injections damage tissue. The fibroblasts were cultured in 5 mL of D-MEM/F-12 medium in each well of a 6-well culture dish. After 1 hour, we added 5 mL of prepared periarticular injection solution to each well of the study group. To determine the effects of the solution on the fibroblasts, we assessed viability rates at the end of 24, 48, and 72 hours. When we compared the control and the study group, we did not encounter any apoptotic or dying cells in either group, which we interpreted to mean that the periarticular injection solution did not show a lethal effect on fibroblasts. Therefore, we conducted an in vitro and electron microscope study to research this issue. We found that the number and viability of fibroblasts did not change significantly with periarticular injection and thus concluded does not cause cell damage and can safely be used.
| Published in | International Journal of Anesthesia and Clinical Medicine (Volume 9, Issue 2) |
| DOI | 10.11648/j.ijacm.20210902.12 |
| Page(s) | 28-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Periarticular Injection, Hip and Knee Arthroplasty, Analgesia, Fibroblast
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APA Style
Muhammed Sadik Bilgen, Zeynep Kahveci, Ilkin Cavusoglu, Muren Mutlu, Omer Faruk Bilgen, et al. (2021). An Examination of the in Vitro Effects on Fibroblasts of Periarticular Injection Solution Applied in Total Hip and Knee Arthroplasty. International Journal of Anesthesia and Clinical Medicine, 9(2), 28-31. https://doi.org/10.11648/j.ijacm.20210902.12
ACS Style
Muhammed Sadik Bilgen; Zeynep Kahveci; Ilkin Cavusoglu; Muren Mutlu; Omer Faruk Bilgen, et al. An Examination of the in Vitro Effects on Fibroblasts of Periarticular Injection Solution Applied in Total Hip and Knee Arthroplasty. Int. J. Anesth. Clin. Med. 2021, 9(2), 28-31. doi: 10.11648/j.ijacm.20210902.12
AMA Style
Muhammed Sadik Bilgen, Zeynep Kahveci, Ilkin Cavusoglu, Muren Mutlu, Omer Faruk Bilgen, et al. An Examination of the in Vitro Effects on Fibroblasts of Periarticular Injection Solution Applied in Total Hip and Knee Arthroplasty. Int J Anesth Clin Med. 2021;9(2):28-31. doi: 10.11648/j.ijacm.20210902.12
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Download@article{10.11648/j.ijacm.20210902.12,
author = {Muhammed Sadik Bilgen and Zeynep Kahveci and Ilkin Cavusoglu and Muren Mutlu and Omer Faruk Bilgen and Aysun Yilmazlar},
title = {An Examination of the in Vitro Effects on Fibroblasts of Periarticular Injection Solution Applied in Total Hip and Knee Arthroplasty},
journal = {International Journal of Anesthesia and Clinical Medicine},
volume = {9},
number = {2},
pages = {28-31},
doi = {10.11648/j.ijacm.20210902.12},
url = {https://doi.org/10.11648/j.ijacm.20210902.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijacm.20210902.12},
abstract = {The number of indications for total hip and knee surgery is increasing. The anesthesia and analgesia techniques are very important and effectivity on success of total hip and knee arthroplasty. Multimodal analgesia makes a great contribution to pain treatment after surgery. Periarticular injection of an analgesic solution is a component of multimodal analgesia. However, it has long been unclear whether such injections damage tissue. The fibroblasts were cultured in 5 mL of D-MEM/F-12 medium in each well of a 6-well culture dish. After 1 hour, we added 5 mL of prepared periarticular injection solution to each well of the study group. To determine the effects of the solution on the fibroblasts, we assessed viability rates at the end of 24, 48, and 72 hours. When we compared the control and the study group, we did not encounter any apoptotic or dying cells in either group, which we interpreted to mean that the periarticular injection solution did not show a lethal effect on fibroblasts. Therefore, we conducted an in vitro and electron microscope study to research this issue. We found that the number and viability of fibroblasts did not change significantly with periarticular injection and thus concluded does not cause cell damage and can safely be used.},
year = {2021}
}
TY - JOUR T1 - An Examination of the in Vitro Effects on Fibroblasts of Periarticular Injection Solution Applied in Total Hip and Knee Arthroplasty AU - Muhammed Sadik Bilgen AU - Zeynep Kahveci AU - Ilkin Cavusoglu AU - Muren Mutlu AU - Omer Faruk Bilgen AU - Aysun Yilmazlar Y1 - 2021/07/08 PY - 2021 N1 - https://doi.org/10.11648/j.ijacm.20210902.12 DO - 10.11648/j.ijacm.20210902.12 T2 - International Journal of Anesthesia and Clinical Medicine JF - International Journal of Anesthesia and Clinical Medicine JO - International Journal of Anesthesia and Clinical Medicine SP - 28 EP - 31 PB - Science Publishing Group SN - 2997-2698 UR - https://doi.org/10.11648/j.ijacm.20210902.12 AB - The number of indications for total hip and knee surgery is increasing. The anesthesia and analgesia techniques are very important and effectivity on success of total hip and knee arthroplasty. Multimodal analgesia makes a great contribution to pain treatment after surgery. Periarticular injection of an analgesic solution is a component of multimodal analgesia. However, it has long been unclear whether such injections damage tissue. The fibroblasts were cultured in 5 mL of D-MEM/F-12 medium in each well of a 6-well culture dish. After 1 hour, we added 5 mL of prepared periarticular injection solution to each well of the study group. To determine the effects of the solution on the fibroblasts, we assessed viability rates at the end of 24, 48, and 72 hours. When we compared the control and the study group, we did not encounter any apoptotic or dying cells in either group, which we interpreted to mean that the periarticular injection solution did not show a lethal effect on fibroblasts. Therefore, we conducted an in vitro and electron microscope study to research this issue. We found that the number and viability of fibroblasts did not change significantly with periarticular injection and thus concluded does not cause cell damage and can safely be used. VL - 9 IS - 2 ER -
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