Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos

Feng Guangyu; Xiang Chunhe; Wang Limin; Zhou Ping; Pi Wenhui

doi:doi:10.11648/j.ijast.20230703.12

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Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos

Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui

Published in International Journal of Animal Science and Technology (Volume 7, Issue 3)

Received: 15 September 2023 Accepted: 4 October 2023 Published: 14 October 2023

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Abstract

The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.

Published in	International Journal of Animal Science and Technology (Volume 7, Issue 3)
DOI	10.11648/j.ijast.20230703.12
Page(s)	43-47
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Incubator, In Vitro Culture, Embryo, Sheep

References

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APA Style

Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. (2023). Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. International Journal of Animal Science and Technology, 7(3), 43-47. https://doi.org/10.11648/j.ijast.20230703.12

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ACS Style

Feng Guangyu; Xiang Chunhe; Wang Limin; Zhou Ping; Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int. J. Anim. Sci. Technol. 2023, 7(3), 43-47. doi: 10.11648/j.ijast.20230703.12

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AMA Style

Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int J Anim Sci Technol. 2023;7(3):43-47. doi: 10.11648/j.ijast.20230703.12

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@article{10.11648/j.ijast.20230703.12,
  author = {Feng Guangyu and Xiang Chunhe and Wang Limin and Zhou Ping and Pi Wenhui},
  title = {Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos},
  journal = {International Journal of Animal Science and Technology},
  volume = {7},
  number = {3},
  pages = {43-47},
  doi = {10.11648/j.ijast.20230703.12},
  url = {https://doi.org/10.11648/j.ijast.20230703.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20230703.12},
  abstract = {The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.},
 year = {2023}
}

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TY  - JOUR
T1  - Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos
AU  - Feng Guangyu
AU  - Xiang Chunhe
AU  - Wang Limin
AU  - Zhou Ping
AU  - Pi Wenhui
Y1  - 2023/10/14
PY  - 2023
N1  - https://doi.org/10.11648/j.ijast.20230703.12
DO  - 10.11648/j.ijast.20230703.12
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 43
EP  - 47
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20230703.12
AB  - The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.
VL  - 7
IS  - 3
ER  -

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Author Information

Feng Guangyu

College of Animal Science and Technology, Shihezi University, Shihezi, China
Xiang Chunhe

College of Animal Science and Technology, Shihezi University, Shihezi, China
Wang Limin

State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China
Zhou Ping

State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China
Pi Wenhui

College of Animal Science and Technology, Shihezi University, Shihezi, China

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Plain Text BibTeX RIS

APA Style

Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. (2023). Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. International Journal of Animal Science and Technology, 7(3), 43-47. https://doi.org/10.11648/j.ijast.20230703.12

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ACS Style

Feng Guangyu; Xiang Chunhe; Wang Limin; Zhou Ping; Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int. J. Anim. Sci. Technol. 2023, 7(3), 43-47. doi: 10.11648/j.ijast.20230703.12

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AMA Style

Feng Guangyu, Xiang Chunhe, Wang Limin, Zhou Ping, Pi Wenhui. Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos. Int J Anim Sci Technol. 2023;7(3):43-47. doi: 10.11648/j.ijast.20230703.12

Copy | Download

@article{10.11648/j.ijast.20230703.12,
  author = {Feng Guangyu and Xiang Chunhe and Wang Limin and Zhou Ping and Pi Wenhui},
  title = {Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos},
  journal = {International Journal of Animal Science and Technology},
  volume = {7},
  number = {3},
  pages = {43-47},
  doi = {10.11648/j.ijast.20230703.12},
  url = {https://doi.org/10.11648/j.ijast.20230703.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijast.20230703.12},
  abstract = {The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.},
 year = {2023}
}

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TY  - JOUR
T1  - Effect of Stainless Steel Tank Incubation System on Development of Ovine Embryos
AU  - Feng Guangyu
AU  - Xiang Chunhe
AU  - Wang Limin
AU  - Zhou Ping
AU  - Pi Wenhui
Y1  - 2023/10/14
PY  - 2023
N1  - https://doi.org/10.11648/j.ijast.20230703.12
DO  - 10.11648/j.ijast.20230703.12
T2  - International Journal of Animal Science and Technology
JF  - International Journal of Animal Science and Technology
JO  - International Journal of Animal Science and Technology
SP  - 43
EP  - 47
PB  - Science Publishing Group
SN  - 2640-1312
UR  - https://doi.org/10.11648/j.ijast.20230703.12
AB  - The utilization of separate culture chambers serves a dual purpose: it not only facilitates the creation of a hypoxic environment but also contributes to the establishment of a consistently stable local environment. This, in turn, mitigates environmental stress factors. The primary objective of this experiment was to assess the developmental potential of sheep embryos before their implantation. This assessment was conducted within the confines of 304 stainless steel culture chambers, offering a viable alternative for maintaining a stable in vitro embryo culture environment. The experiment involved a comparative analysis between the development outcomes of parthenogenetic and reconstituted sheep embryos cultured in both stainless steel tanks or MIC-101 incubators over a period of 7 days. Notably, the blastocyst rates for parthenogenetic embryos were 26.45% and 24.84% in stainless steel tanks and MIC-101 incubators, respectively, with no statistically significant difference (P>0.05) between them. Similarly, the blastocyst rates for reconstituted embryos stood at 16.07% and 16.84% (P>0.05) for stainless steel tanks and MIC-101 incubators, respectively. It is worth highlighting that the incubation system, comprising a 304 stainless steel tank, a standard gas mixture, and a thermostat, presents an in vitro culture system with the advantages of cost-effectiveness, environmental stability, and efficacy.
VL  - 7
IS  - 3
ER  -

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