Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs

Ahonsi Cyril Ohikhatemen; Ovobovwori Solomon Uvietobore; Etatuvie Samuel Oghene

doi:doi:10.11648/j.ijhnm.20230901.12

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Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs

Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene

Published in International Journal of Homeopathy & Natural Medicines (Volume 9, Issue 1)

Received: 31 January 2023 Accepted: 27 February 2023 Published: 10 June 2023

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Abstract

The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.

Published in	International Journal of Homeopathy & Natural Medicines (Volume 9, Issue 1)
DOI	10.11648/j.ijhnm.20230901.12
Page(s)	6-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Polyherbal Formulation (PHF), Total Phenolic Content (TPC), Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazil, Hydroxyl (OH) Radical Scavenging Activity

References

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Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. (2023). Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. International Journal of Homeopathy & Natural Medicines, 9(1), 6-14. https://doi.org/10.11648/j.ijhnm.20230901.12

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Ahonsi Cyril Ohikhatemen; Ovobovwori Solomon Uvietobore; Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int. J. Homeopathy Nat. Med. 2023, 9(1), 6-14. doi: 10.11648/j.ijhnm.20230901.12

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AMA Style

Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int J Homeopathy Nat Med. 2023;9(1):6-14. doi: 10.11648/j.ijhnm.20230901.12

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@article{10.11648/j.ijhnm.20230901.12,
  author = {Ahonsi Cyril Ohikhatemen and Ovobovwori Solomon Uvietobore and Etatuvie Samuel Oghene},
  title = {Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs},
  journal = {International Journal of Homeopathy & Natural Medicines},
  volume = {9},
  number = {1},
  pages = {6-14},
  doi = {10.11648/j.ijhnm.20230901.12},
  url = {https://doi.org/10.11648/j.ijhnm.20230901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijhnm.20230901.12},
  abstract = {The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.},
 year = {2023}
}

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TY  - JOUR
T1  - Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs
AU  - Ahonsi Cyril Ohikhatemen
AU  - Ovobovwori Solomon Uvietobore
AU  - Etatuvie Samuel Oghene
Y1  - 2023/06/10
PY  - 2023
N1  - https://doi.org/10.11648/j.ijhnm.20230901.12
DO  - 10.11648/j.ijhnm.20230901.12
T2  - International Journal of Homeopathy & Natural Medicines
JF  - International Journal of Homeopathy & Natural Medicines
JO  - International Journal of Homeopathy & Natural Medicines
SP  - 6
EP  - 14
PB  - Science Publishing Group
SN  - 2472-2316
UR  - https://doi.org/10.11648/j.ijhnm.20230901.12
AB  - The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.
VL  - 9
IS  - 1
ER  -

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Ahonsi Cyril Ohikhatemen

Department of Product Development/Quality Assurance, Nigeria Natural Medicine Development Agency, Federal Ministry of Science, Technology and Innovation, Abuja, Nigeria
Ovobovwori Solomon Uvietobore

Department of Product Development/Quality Assurance, Nigeria Natural Medicine Development Agency, Federal Ministry of Science, Technology and Innovation, Abuja, Nigeria
Etatuvie Samuel Oghene

Department of Product Development/Quality Assurance, Nigeria Natural Medicine Development Agency, Federal Ministry of Science, Technology and Innovation, Abuja, Nigeria

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Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. (2023). Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. International Journal of Homeopathy & Natural Medicines, 9(1), 6-14. https://doi.org/10.11648/j.ijhnm.20230901.12

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Ahonsi Cyril Ohikhatemen; Ovobovwori Solomon Uvietobore; Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int. J. Homeopathy Nat. Med. 2023, 9(1), 6-14. doi: 10.11648/j.ijhnm.20230901.12

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AMA Style

Ahonsi Cyril Ohikhatemen, Ovobovwori Solomon Uvietobore, Etatuvie Samuel Oghene. Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs. Int J Homeopathy Nat Med. 2023;9(1):6-14. doi: 10.11648/j.ijhnm.20230901.12

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@article{10.11648/j.ijhnm.20230901.12,
  author = {Ahonsi Cyril Ohikhatemen and Ovobovwori Solomon Uvietobore and Etatuvie Samuel Oghene},
  title = {Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs},
  journal = {International Journal of Homeopathy & Natural Medicines},
  volume = {9},
  number = {1},
  pages = {6-14},
  doi = {10.11648/j.ijhnm.20230901.12},
  url = {https://doi.org/10.11648/j.ijhnm.20230901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijhnm.20230901.12},
  abstract = {The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.},
 year = {2023}
}

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TY  - JOUR
T1  - Phytochemical and Antioxidant Activities of a Polyherbal Formulation from Selected Medicinal Herbs
AU  - Ahonsi Cyril Ohikhatemen
AU  - Ovobovwori Solomon Uvietobore
AU  - Etatuvie Samuel Oghene
Y1  - 2023/06/10
PY  - 2023
N1  - https://doi.org/10.11648/j.ijhnm.20230901.12
DO  - 10.11648/j.ijhnm.20230901.12
T2  - International Journal of Homeopathy & Natural Medicines
JF  - International Journal of Homeopathy & Natural Medicines
JO  - International Journal of Homeopathy & Natural Medicines
SP  - 6
EP  - 14
PB  - Science Publishing Group
SN  - 2472-2316
UR  - https://doi.org/10.11648/j.ijhnm.20230901.12
AB  - The present study aimed to investigate the phytochemical and antioxidant activities of a polyherbal formulation (PHF) obtained from the Nigerian Natural Medicine Development Agency (NNMDA) using In vitro methods. Ethanolic extract of the polyhebal formulation was prepared by the Soxhlation process, Phytochemical screening, Total Phenolic Content (TPC) and Total flavonoid content (TFC) of the extracts were estimated using standard methods. Extracts were analyzed for its antioxidant potential using DPPH (1,1-diphenyl-2-picrylhydrazil), FRAP (Ferric Reducing Antioxidant Power), OH (Hydroxyl radical scavenging activity), NO (Nitric oxide scavenging activity), ABTS {2,2’-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid)}, and SRS (Superoxide Radical Scavenging activity) methods to assay their free scavenging activity. Results shows that PHF contains Flavonoids (QE) 15.61±1.95, Total phenol (GAE) 25.24±3.56, preliminary phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, glycosides, Anthraquinone, Terpenoid, Steroid, Phlobatannins and Coumarins. Antioxidant study for DPPH, FRAP, OH, NO, ABTS, and SRS of PHF were 86.09±1.58, 66.95±0.03, 80.89±0.10, 68.13±0.16, 65.54±0.55, and 69.69±1.12, at 250 µg/mL respectively. When compared with known standards PHF possess high antioxidant activity in a concentration dependant manner. IC50 values of drug combination exhibited higher antioxidant potential in DPPH (26.40± 0.56 µg/mL) and ABTS (28.91±0.34 µg/mL), which may be due to the combined activity of the individual plant extracts with its high phenolic and flavonoid content. these findings may provide efficient, supportive data on the use of medicinal herbs for treatment of numerous health ailments associated with the accumulation of harmful free radicals and reactive oxygen species.
VL  - 9
IS  - 1
ER  -

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