Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus

Jing Yang; Minhui Wu; Yi Wu; Runzhang He; Yating Nong; Chun Zhang; Sheng Zhou

doi:doi:10.11648/j.ijovs.20220701.17

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Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus

Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, Chun Zhang, Sheng Zhou

Published in International Journal of Ophthalmology & Visual Science (Volume 7, Issue 1)

Received: 15 February 2022 Accepted: 4 March 2022 Published: 12 March 2022

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Abstract

Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm² versus 4057.81 ± 316.29 cells/mm² versus 3522.65 ± 978.10 cells/mm²). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.

Published in	International Journal of Ophthalmology & Visual Science (Volume 7, Issue 1)
DOI	10.11648/j.ijovs.20220701.17
Page(s)	40-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Confocal, Keratoconus, Epithelial Cell, Fold

References

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APA Style

Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. (2022). Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. International Journal of Ophthalmology & Visual Science, 7(1), 40-44. https://doi.org/10.11648/j.ijovs.20220701.17

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ACS Style

Jing Yang; Minhui Wu; Yi Wu; Runzhang He; Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int. J. Ophthalmol. Vis. Sci. 2022, 7(1), 40-44. doi: 10.11648/j.ijovs.20220701.17

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AMA Style

Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int J Ophthalmol Vis Sci. 2022;7(1):40-44. doi: 10.11648/j.ijovs.20220701.17

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@article{10.11648/j.ijovs.20220701.17,
  author = {Jing Yang and Minhui Wu and Yi Wu and Runzhang He and Yating Nong and Chun Zhang and Sheng Zhou},
  title = {Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {7},
  number = {1},
  pages = {40-44},
  doi = {10.11648/j.ijovs.20220701.17},
  url = {https://doi.org/10.11648/j.ijovs.20220701.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20220701.17},
  abstract = {Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.},
 year = {2022}
}

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TY - JOUR
T1 - Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus
AU - Jing Yang
AU - Minhui Wu
AU - Yi Wu
AU - Runzhang He
AU - Yating Nong
AU - Chun Zhang
AU - Sheng Zhou
Y1 - 2022/03/12
PY - 2022
N1 - https://doi.org/10.11648/j.ijovs.20220701.17
DO - 10.11648/j.ijovs.20220701.17
T2 - International Journal of Ophthalmology & Visual Science
JF - International Journal of Ophthalmology & Visual Science
JO - International Journal of Ophthalmology & Visual Science
SP - 40
EP - 44
PB - Science Publishing Group
SN - 2637-3858
UR - https://doi.org/10.11648/j.ijovs.20220701.17
AB - Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.
VL - 7
IS - 1
ER -

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Author Information

Jing Yang

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Minhui Wu

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Yi Wu

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Runzhang He

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Yating Nong

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Chun Zhang

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
Sheng Zhou

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

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Plain Text BibTeX RIS

APA Style

Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. (2022). Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. International Journal of Ophthalmology & Visual Science, 7(1), 40-44. https://doi.org/10.11648/j.ijovs.20220701.17

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ACS Style

Jing Yang; Minhui Wu; Yi Wu; Runzhang He; Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int. J. Ophthalmol. Vis. Sci. 2022, 7(1), 40-44. doi: 10.11648/j.ijovs.20220701.17

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AMA Style

Jing Yang, Minhui Wu, Yi Wu, Runzhang He, Yating Nong, et al. Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus. Int J Ophthalmol Vis Sci. 2022;7(1):40-44. doi: 10.11648/j.ijovs.20220701.17

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@article{10.11648/j.ijovs.20220701.17,
  author = {Jing Yang and Minhui Wu and Yi Wu and Runzhang He and Yating Nong and Chun Zhang and Sheng Zhou},
  title = {Cornea Confocal Microscopy Study of Patients with Preclinical Keratoconus},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {7},
  number = {1},
  pages = {40-44},
  doi = {10.11648/j.ijovs.20220701.17},
  url = {https://doi.org/10.11648/j.ijovs.20220701.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20220701.17},
  abstract = {Purpose: To report the difference of corneal confocal microscopy examination in patients with preclinical keratoconus or clinical keratoconus. Methods: 8 unilateral keratoconus patients were examined by confocal microscopy before they had cross-linking surgery. As control groups, 23 patients with myopia and 13 patients with keratoconus in both eyes were also examined by confocal microscopy. Then we used Image J to compared the density of epithelium cell, length of cornea nerve fiber, density of Langerhans cell, density of keratocyte in shallow, middle, deep stroma, and the width and grayscale value of fold near Descement membrane for each group. Result: There was differences in corneal epithelial cell count between three groups (4358.27 ± 635.14 cells/mm2 versus 4057.81 ± 316.29 cells/mm2 versus 3522.65 ± 978.10 cells/mm2). The density of keratocyte in stroma showed a tendency to decrease with increasing depth. The density of keratocyte in keratoconus was less than that in normal eyes. The fold showed much wider and daker in keratoconus eyes than in normal eyes. The density of Langerhans cell was more in keratoconus group than it in normal group. Conclusion: We first report the differences in corneal epithelial cell count and fold near Descement membrane. It might provide a new way to diagnose early keratoconus. The difference of density of Langerhans cell suggested us the possibility of inflammation in keratoconus.},
 year = {2022}
}

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