Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso.
| Published in | Journal of Diseases and Medicinal Plants (Volume 9, Issue 4) |
| DOI | 10.11648/j.jdmp.20230904.14 |
| Page(s) | 148-155 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Cytotoxic, Antiradical, Anti-lipoxygenase, Acti-plus
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APA Style
Donald Wilfried Adico, M., Bayala, B., Valérie Elvira Jean Télesphore Bazié, B., Traoré, L., Théophane Yonli, A., et al. (2023). Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. Journal of Diseases and Medicinal Plants, 9(4), 148-155. https://doi.org/10.11648/j.jdmp.20230904.14
ACS Style
Donald Wilfried Adico, M.; Bayala, B.; Valérie Elvira Jean Télesphore Bazié, B.; Traoré, L.; Théophane Yonli, A., et al. Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. J. Dis. Med. Plants 2023, 9(4), 148-155. doi: 10.11648/j.jdmp.20230904.14
AMA Style
Donald Wilfried Adico M, Bayala B, Valérie Elvira Jean Télesphore Bazié B, Traoré L, Théophane Yonli A, et al. Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso. J Dis Med Plants. 2023;9(4):148-155. doi: 10.11648/j.jdmp.20230904.14
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Download@article{10.11648/j.jdmp.20230904.14,
author = {Marc Donald Wilfried Adico and Bagora Bayala and Bapio Valérie Elvira Jean Télesphore Bazié and Lassina Traoré and Albert Théophane Yonli and Jacques Simporé},
title = {Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso},
journal = {Journal of Diseases and Medicinal Plants},
volume = {9},
number = {4},
pages = {148-155},
doi = {10.11648/j.jdmp.20230904.14},
url = {https://doi.org/10.11648/j.jdmp.20230904.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230904.14},
abstract = {Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso.
},
year = {2023}
}
TY - JOUR T1 - Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «Acti-plus», a Recipe Based on Medicinal Plants from Burkina Faso AU - Marc Donald Wilfried Adico AU - Bagora Bayala AU - Bapio Valérie Elvira Jean Télesphore Bazié AU - Lassina Traoré AU - Albert Théophane Yonli AU - Jacques Simporé Y1 - 2023/12/11 PY - 2023 N1 - https://doi.org/10.11648/j.jdmp.20230904.14 DO - 10.11648/j.jdmp.20230904.14 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 148 EP - 155 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20230904.14 AB - Medicinal plants are increasingly emerging as a real alternative that can contribute to the fight against cancer. The purpose of this study was to evaluate in vitro the antiradical, anti-lipoxygenase and cytotoxic properties on cancerous cells in culture of the medicinal recipe «Acti-plus». The extraction of «Acti-plus» was carried out according the instructions of the traditional health practitioner. Phytochemical screening, as well as the determination of polyphenols and total flavonoids were performed using characterization and assay tests by spectrometry as well as by high performance thin layer chromatography (HPTLC). Antiradical tests for trapping DPPH and ABTS radicals were used to measure the antioxidant potential. Anti-lipoxygenase activity was studied using 15-lipoxygenase (15-LOX) inhibition test. The cytotoxic activity of aqueous extract of «Acti-plus» was evaluated in vitro on human cancers cell using MTT test. The results of the study showed that aqueous extract of «Acti-plus» contains flavonoids, coumarins and tannins. Total phenolic compounds (TPC) and total flavonoids (TFC) were 61.95 ± 0.01 mg GAE/g dry extract and 43.01 ± 0.05 mg QE/g dry extract respectively. The extract exhibited excellent scavenging capacity of DPPH (IC50 = 9.33 ± 1.53 µg/mL) and ABTS (IC50 = 53.33 ± 0.89 µg/mL) radicals antioxidant potential. The study also found that the extract moderately inhibits 15-lipoxygenase at 100 µg/mL. Aqueous extract of «Acti-plus» inhibited in a concentration-dependent manner the proliferation of the three cell lines with best activity (IC50 = 0.317 ± 0.02 mg/mL) on prostate cancer PC-3 cells. These properties give a rational basis for the use of the recipe «Acti-plus» in traditional medicine in Burkina Faso. VL - 9 IS - 4 ER -
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso; Higher Normal School (ENS), Koudougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
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