Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire

Guinagui N’Doua Bertrand; Yeo Gnenakan; Tuo Seydou; Kouda Affiba Genevieve; Lehi Malidy Irenee; Danon Aubin Silvere Djiwha; Fatogoma Sorho

doi:doi:10.11648/j.jps.20221006.16

Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire

Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, Danon Aubin Silvere Djiwha, Fatogoma Sorho

Published in Journal of Plant Sciences (Volume 10, Issue 6)

Received: 24 September 2022 Accepted: 28 October 2022 Published: 8 December 2022

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Abstract

Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.

Published in	Journal of Plant Sciences (Volume 10, Issue 6)
DOI	10.11648/j.jps.20221006.16
Page(s)	235-240
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

RYMV, Resistance-Breaking, Gigante, Tog 5674, Côte d'Ivoire

References

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APA Style

Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. (2022). Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. Journal of Plant Sciences, 10(6), 235-240. https://doi.org/10.11648/j.jps.20221006.16

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ACS Style

Guinagui N’Doua Bertrand; Yeo Gnenakan; Tuo Seydou; Kouda Affiba Genevieve; Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J. Plant Sci. 2022, 10(6), 235-240. doi: 10.11648/j.jps.20221006.16

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AMA Style

Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J Plant Sci. 2022;10(6):235-240. doi: 10.11648/j.jps.20221006.16

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@article{10.11648/j.jps.20221006.16,
  author = {Guinagui N’Doua Bertrand and Yeo Gnenakan and Tuo Seydou and Kouda Affiba Genevieve and Lehi Malidy Irenee and Danon Aubin Silvere Djiwha and Fatogoma Sorho},
  title = {Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {6},
  pages = {235-240},
  doi = {10.11648/j.jps.20221006.16},
  url = {https://doi.org/10.11648/j.jps.20221006.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.16},
  abstract = {Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.},
 year = {2022}
}

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TY - JOUR
T1 - Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire
AU - Guinagui N’Doua Bertrand
AU - Yeo Gnenakan
AU - Tuo Seydou
AU - Kouda Affiba Genevieve
AU - Lehi Malidy Irenee
AU - Danon Aubin Silvere Djiwha
AU - Fatogoma Sorho
Y1 - 2022/12/08
PY - 2022
N1 - https://doi.org/10.11648/j.jps.20221006.16
DO - 10.11648/j.jps.20221006.16
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 235
EP - 240
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20221006.16
AB - Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.
VL - 10
IS - 6
ER -

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Author Information

Guinagui N’Doua Bertrand

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Yeo Gnenakan

National Center for Agricultural Research, Ferkessedougou Research Station, Ferkessedougou, C?te d’Ivoire
Tuo Seydou

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Kouda Affiba Genevieve

Afrique Pesage, Abidjan, C?te d’Ivoire
Lehi Malidy Irenee

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Danon Aubin Silvere Djiwha

Department of Entomology, Faculty of Natural Sciences, Nangui Abrogoua University, Abidjan, C?te d’Ivoire
Fatogoma Sorho

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Pedagogical and Research Unit of Plant Physiology and Pathology, UFR Biosciences, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

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Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. (2022). Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. Journal of Plant Sciences, 10(6), 235-240. https://doi.org/10.11648/j.jps.20221006.16

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ACS Style

Guinagui N’Doua Bertrand; Yeo Gnenakan; Tuo Seydou; Kouda Affiba Genevieve; Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J. Plant Sci. 2022, 10(6), 235-240. doi: 10.11648/j.jps.20221006.16

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AMA Style

Guinagui N’Doua Bertrand, Yeo Gnenakan, Tuo Seydou, Kouda Affiba Genevieve, Lehi Malidy Irenee, et al. Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire. J Plant Sci. 2022;10(6):235-240. doi: 10.11648/j.jps.20221006.16

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@article{10.11648/j.jps.20221006.16,
  author = {Guinagui N’Doua Bertrand and Yeo Gnenakan and Tuo Seydou and Kouda Affiba Genevieve and Lehi Malidy Irenee and Danon Aubin Silvere Djiwha and Fatogoma Sorho},
  title = {Identification of Resistance-Breaking RYMV Isolates in Four Agro-Ecological Zones of Côte d’Ivoire},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {6},
  pages = {235-240},
  doi = {10.11648/j.jps.20221006.16},
  url = {https://doi.org/10.11648/j.jps.20221006.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.16},
  abstract = {Rice is the third most produced cereal in the world after maize and wheat. In Côte d'Ivoire, rice is the main food of the population. However, despite its importance, rice cultivation is subject to biotic constraints, including rice yellow mottle virus (RYMV), which is the most damaging viral disease of rice native to Africa. RYMV is a highly variable virus. This study aims to assess the ability of RYMV isolates to overcome the resistance-breaking genes RYMV1, RYMV2 and RYMV3. Sixteen RYMV isolates from Côte d'Ivoire representative of the agro-ecological zones (AEZ) visited and the varieties Bouake 189, Gigante, Tog 5681, Tog 5674, Tog 7291 and Tog 5307, carrying respectively rymv1-1 rymv1-2, rymv1-3, rymv1-5, RYMV2 and RYMV3 alleles were used were used. Varieties were sown in trays filled with sterilized soil so as to keep six plants per variety, i.e. 36 plants per tray per isolate. Rice plants were inoculated mechanically 14 days after sowing. Leaves of infected plants were collected individually per variety 45 days after inoculation in order to determine the virus concentration by serological DAS-ELISA tests. Asymptomatic infections were also investigated. The results showed that 37.5% of the tested isolates broke only the rymv1-2 gene with the presence of apparent symptoms. However, 12.5% of the isolates are infected both Gigante and Tog5674. None of the 16 isolates tested could induce symptoms in Tog5703, Tog5681 and Tog7291. Furthermore, only Tog7291 are showed the presence of the virus without apparent symptoms on rice leaves after inoculation. The identification of such isolates in rice-growing areas raises a serious threat to Ivorian rice production and calls for surveillance of this disease in Côte d'Ivoire.},
 year = {2022}
}

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