One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.
| Published in | Modern Chemistry (Volume 11, Issue 3) |
| DOI | 10.11648/j.mc.20231103.12 |
| Page(s) | 60-69 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
In Vitro Antibacterial, Anti Diabetic, Agar Disk Diffusion, Rosemary Leave, Phytochemical Screening, Alpha-Amylase Inhibitory Assay
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APA Style
Tesfahun Dagnaw, Lomi Abayneh, Alebachew Molla, Tangut Masreshaw, Hailemariam Assefa, et al. (2023). Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Modern Chemistry, 11(3), 60-69. https://doi.org/10.11648/j.mc.20231103.12
ACS Style
Tesfahun Dagnaw; Lomi Abayneh; Alebachew Molla; Tangut Masreshaw; Hailemariam Assefa, et al. Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Mod. Chem. 2023, 11(3), 60-69. doi: 10.11648/j.mc.20231103.12
AMA Style
Tesfahun Dagnaw, Lomi Abayneh, Alebachew Molla, Tangut Masreshaw, Hailemariam Assefa, et al. Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Mod Chem. 2023;11(3):60-69. doi: 10.11648/j.mc.20231103.12
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author = {Tesfahun Dagnaw and Lomi Abayneh and Alebachew Molla and Tangut Masreshaw and Hailemariam Assefa and Liyew Yizengaw},
title = {Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary)},
journal = {Modern Chemistry},
volume = {11},
number = {3},
pages = {60-69},
doi = {10.11648/j.mc.20231103.12},
url = {https://doi.org/10.11648/j.mc.20231103.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.mc.20231103.12},
abstract = {One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.
},
year = {2023}
}
TY - JOUR T1 - Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary) AU - Tesfahun Dagnaw AU - Lomi Abayneh AU - Alebachew Molla AU - Tangut Masreshaw AU - Hailemariam Assefa AU - Liyew Yizengaw Y1 - 2023/10/31 PY - 2023 N1 - https://doi.org/10.11648/j.mc.20231103.12 DO - 10.11648/j.mc.20231103.12 T2 - Modern Chemistry JF - Modern Chemistry JO - Modern Chemistry SP - 60 EP - 69 PB - Science Publishing Group SN - 2329-180X UR - https://doi.org/10.11648/j.mc.20231103.12 AB - One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts. VL - 11 IS - 3 ER -
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