Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model

Jaianand Kannaiyan; Pavankumar Shukla; Firdosh Mahuvawalla

doi:doi:10.11648/j.rs.20220702.12

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Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model

Jaianand Kannaiyan, Pavankumar Shukla, Firdosh Mahuvawalla

Published in Rehabilitation Science (Volume 7, Issue 2)

Received: 16 March 2022 Accepted: 11 April 2022 Published: 12 May 2022

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Abstract

In-vitro and in-vivo investigations using ex vivo-expanded human Wharton’s jelly-derived mesenchymal stromal cells (WJMSCs) isolated from single donors has shown therapeutic advantages. Clinical grade expansion, safety, and acute toxicity of Wharton’s jelly-derived mesenchymal stromal cells isolated from five healthy donors were evaluated in Wistar Albino Rats via intravenous and subcutaneous routes in this study. The expression of several cell surface markers, differentiation capacity, and immunomodulatory activities of the isolated WJMSCs were all evaluated. In a series of preclinical investigations, the clinical grade expanded cells were given to rodents by intravenous and subcutaneous methods to examine their in-vivo safety, overall toxicity, and tumorigenic potential. We confirmed that WJ cells exhibit morphological and phenotypic features that are comparable to MSCs. In rats, the study found no mortality, aberrant clinical symptoms, or significant pathological alterations at varied dosages administered intravenously or subcutaneously. Furthermore, rats tolerated repeated treatment of WJMSCs well, with no evidence of prenatal harm in the same animal species. The WJMSCs were shown to be non-toxic, non-teratogenic, and non-tumorigenic in Wistar Albino Rats. More research is needed to see if it can be safely supplied to human patients.

Published in	Rehabilitation Science (Volume 7, Issue 2)
DOI	10.11648/j.rs.20220702.12
Page(s)	20-28
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Wharton's Jelly, Umbilical Cord Mesenchymal Stromal Cells, Intravenous, Subcutaneous, Safety, Toxicity

References

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APA Style

Jaianand Kannaiyan, Pavankumar Shukla, Firdosh Mahuvawalla. (2022). Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabilitation Science, 7(2), 20-28. https://doi.org/10.11648/j.rs.20220702.12

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ACS Style

Jaianand Kannaiyan; Pavankumar Shukla; Firdosh Mahuvawalla. Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabil. Sci. 2022, 7(2), 20-28. doi: 10.11648/j.rs.20220702.12

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AMA Style

Jaianand Kannaiyan, Pavankumar Shukla, Firdosh Mahuvawalla. Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabil Sci. 2022;7(2):20-28. doi: 10.11648/j.rs.20220702.12

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@article{10.11648/j.rs.20220702.12,
  author = {Jaianand Kannaiyan and Pavankumar Shukla and Firdosh Mahuvawalla},
  title = {Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model},
  journal = {Rehabilitation Science},
  volume = {7},
  number = {2},
  pages = {20-28},
  doi = {10.11648/j.rs.20220702.12},
  url = {https://doi.org/10.11648/j.rs.20220702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20220702.12},
  abstract = {In-vitro and in-vivo investigations using ex vivo-expanded human Wharton’s jelly-derived mesenchymal stromal cells (WJMSCs) isolated from single donors has shown therapeutic advantages. Clinical grade expansion, safety, and acute toxicity of Wharton’s jelly-derived mesenchymal stromal cells isolated from five healthy donors were evaluated in Wistar Albino Rats via intravenous and subcutaneous routes in this study. The expression of several cell surface markers, differentiation capacity, and immunomodulatory activities of the isolated WJMSCs were all evaluated. In a series of preclinical investigations, the clinical grade expanded cells were given to rodents by intravenous and subcutaneous methods to examine their in-vivo safety, overall toxicity, and tumorigenic potential. We confirmed that WJ cells exhibit morphological and phenotypic features that are comparable to MSCs. In rats, the study found no mortality, aberrant clinical symptoms, or significant pathological alterations at varied dosages administered intravenously or subcutaneously. Furthermore, rats tolerated repeated treatment of WJMSCs well, with no evidence of prenatal harm in the same animal species. The WJMSCs were shown to be non-toxic, non-teratogenic, and non-tumorigenic in Wistar Albino Rats. More research is needed to see if it can be safely supplied to human patients.},
 year = {2022}
}

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TY  - JOUR
T1  - Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model
AU  - Jaianand Kannaiyan
AU  - Pavankumar Shukla
AU  - Firdosh Mahuvawalla
Y1  - 2022/05/12
PY  - 2022
N1  - https://doi.org/10.11648/j.rs.20220702.12
DO  - 10.11648/j.rs.20220702.12
T2  - Rehabilitation Science
JF  - Rehabilitation Science
JO  - Rehabilitation Science
SP  - 20
EP  - 28
PB  - Science Publishing Group
SN  - 2637-594X
UR  - https://doi.org/10.11648/j.rs.20220702.12
AB  - In-vitro and in-vivo investigations using ex vivo-expanded human Wharton’s jelly-derived mesenchymal stromal cells (WJMSCs) isolated from single donors has shown therapeutic advantages. Clinical grade expansion, safety, and acute toxicity of Wharton’s jelly-derived mesenchymal stromal cells isolated from five healthy donors were evaluated in Wistar Albino Rats via intravenous and subcutaneous routes in this study. The expression of several cell surface markers, differentiation capacity, and immunomodulatory activities of the isolated WJMSCs were all evaluated. In a series of preclinical investigations, the clinical grade expanded cells were given to rodents by intravenous and subcutaneous methods to examine their in-vivo safety, overall toxicity, and tumorigenic potential. We confirmed that WJ cells exhibit morphological and phenotypic features that are comparable to MSCs. In rats, the study found no mortality, aberrant clinical symptoms, or significant pathological alterations at varied dosages administered intravenously or subcutaneously. Furthermore, rats tolerated repeated treatment of WJMSCs well, with no evidence of prenatal harm in the same animal species. The WJMSCs were shown to be non-toxic, non-teratogenic, and non-tumorigenic in Wistar Albino Rats. More research is needed to see if it can be safely supplied to human patients.
VL  - 7
IS  - 2
ER  -

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Author Information

Jaianand Kannaiyan

Research and Development, Cell Cure Therapeutics, Coimbatore, India
Pavankumar Shukla

Regeneracion Corporation, Vigan City, Philippines
Firdosh Mahuvawalla

Regeneracion Corporation, Vigan City, Philippines

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APA Style

Jaianand Kannaiyan, Pavankumar Shukla, Firdosh Mahuvawalla. (2022). Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabilitation Science, 7(2), 20-28. https://doi.org/10.11648/j.rs.20220702.12

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ACS Style

Jaianand Kannaiyan; Pavankumar Shukla; Firdosh Mahuvawalla. Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabil. Sci. 2022, 7(2), 20-28. doi: 10.11648/j.rs.20220702.12

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AMA Style

Jaianand Kannaiyan, Pavankumar Shukla, Firdosh Mahuvawalla. Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model. Rehabil Sci. 2022;7(2):20-28. doi: 10.11648/j.rs.20220702.12

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@article{10.11648/j.rs.20220702.12,
  author = {Jaianand Kannaiyan and Pavankumar Shukla and Firdosh Mahuvawalla},
  title = {Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model},
  journal = {Rehabilitation Science},
  volume = {7},
  number = {2},
  pages = {20-28},
  doi = {10.11648/j.rs.20220702.12},
  url = {https://doi.org/10.11648/j.rs.20220702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.rs.20220702.12},
  abstract = {In-vitro and in-vivo investigations using ex vivo-expanded human Wharton’s jelly-derived mesenchymal stromal cells (WJMSCs) isolated from single donors has shown therapeutic advantages. Clinical grade expansion, safety, and acute toxicity of Wharton’s jelly-derived mesenchymal stromal cells isolated from five healthy donors were evaluated in Wistar Albino Rats via intravenous and subcutaneous routes in this study. The expression of several cell surface markers, differentiation capacity, and immunomodulatory activities of the isolated WJMSCs were all evaluated. In a series of preclinical investigations, the clinical grade expanded cells were given to rodents by intravenous and subcutaneous methods to examine their in-vivo safety, overall toxicity, and tumorigenic potential. We confirmed that WJ cells exhibit morphological and phenotypic features that are comparable to MSCs. In rats, the study found no mortality, aberrant clinical symptoms, or significant pathological alterations at varied dosages administered intravenously or subcutaneously. Furthermore, rats tolerated repeated treatment of WJMSCs well, with no evidence of prenatal harm in the same animal species. The WJMSCs were shown to be non-toxic, non-teratogenic, and non-tumorigenic in Wistar Albino Rats. More research is needed to see if it can be safely supplied to human patients.},
 year = {2022}
}

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TY  - JOUR
T1  - Acute Toxicity Evaluation and Safety Study of Allogeneic Wharton’s Jelly-Derived Mesenchymal Stromal Cells Therapy in the Animal Model
AU  - Jaianand Kannaiyan
AU  - Pavankumar Shukla
AU  - Firdosh Mahuvawalla
Y1  - 2022/05/12
PY  - 2022
N1  - https://doi.org/10.11648/j.rs.20220702.12
DO  - 10.11648/j.rs.20220702.12
T2  - Rehabilitation Science
JF  - Rehabilitation Science
JO  - Rehabilitation Science
SP  - 20
EP  - 28
PB  - Science Publishing Group
SN  - 2637-594X
UR  - https://doi.org/10.11648/j.rs.20220702.12
AB  - In-vitro and in-vivo investigations using ex vivo-expanded human Wharton’s jelly-derived mesenchymal stromal cells (WJMSCs) isolated from single donors has shown therapeutic advantages. Clinical grade expansion, safety, and acute toxicity of Wharton’s jelly-derived mesenchymal stromal cells isolated from five healthy donors were evaluated in Wistar Albino Rats via intravenous and subcutaneous routes in this study. The expression of several cell surface markers, differentiation capacity, and immunomodulatory activities of the isolated WJMSCs were all evaluated. In a series of preclinical investigations, the clinical grade expanded cells were given to rodents by intravenous and subcutaneous methods to examine their in-vivo safety, overall toxicity, and tumorigenic potential. We confirmed that WJ cells exhibit morphological and phenotypic features that are comparable to MSCs. In rats, the study found no mortality, aberrant clinical symptoms, or significant pathological alterations at varied dosages administered intravenously or subcutaneously. Furthermore, rats tolerated repeated treatment of WJMSCs well, with no evidence of prenatal harm in the same animal species. The WJMSCs were shown to be non-toxic, non-teratogenic, and non-tumorigenic in Wistar Albino Rats. More research is needed to see if it can be safely supplied to human patients.
VL  - 7
IS  - 2
ER  -

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