Perpetual Smoking Compromise the Osmotic Fragility of RBC
Mazhar Mushtaq,
Uzma Jabbar,
Mumtaz Begum,
Naim Ahmad Nazami
Issue:
Volume 3, Issue 3, May 2017
Pages:
58-62
Received:
16 May 2017
Accepted:
14 June 2017
Published:
21 July 2017
Abstract: Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.
Abstract: Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and co...
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Classical Methods of Tissue Studies: The Artifacts and the Impact of Transverse Serial Sections
DEH Zhou Patricia,
Koffi DP,
Atto V,
Monteomo GF
Issue:
Volume 3, Issue 3, May 2017
Pages:
63-66
Received:
26 May 2017
Accepted:
19 July 2017
Published:
9 August 2017
Abstract: This work aims to study the mechanism of appearance of the histological alterations inherent in classical methods of tissue study and to give the difficulties related to the incidence of transverse serial sections. 4 lefts and rights testes were taken from two immature rats aged between 11 and 12 days. The testes were taken under anesthesia by ether inhalation. The gonads were fixed in 10% formic aldehyde and dehydrated in alcohol and then included in the paraffin. The ribbon of transverse serial sections of 5 μm were made by a microtome from the upper pole to the lower pole of the testes. The portions of the ribbon were then spread on slides. The smears were stained with hemalun-eosin and then covered with coverslip. The observation of transverse serial sections presented different types of degradation on them caused by technology. This involves deforming the contours of sections characterized by dye deposits, albuginea folds, tearing or destruction of the seminiferous tubes and then the formation of artificial vacuum between the tubes in the testicular parenchyma. It resulted from the stage of section, the loss of the three-dimensional (3D) coherence of the testes, generating the images visualized in a plan in a two-dimensional (2D) plane resulting from the serial histological sections. The results of this study show that the steps of the methods of tissue study are major factors that can condition the success or failure of reading the results of the histological technique. This study invites us to consider artefacts that combine artificial images created by classical methods of tissue study. In addition, images visualized in a plan with two dimensions (2D) do not allow a direct assessment of the spatial configuration. Thus, a better understanding of the structures, a correction of deformities and a three-dimensional reconstitution (3D) could improve compliance.
Abstract: This work aims to study the mechanism of appearance of the histological alterations inherent in classical methods of tissue study and to give the difficulties related to the incidence of transverse serial sections. 4 lefts and rights testes were taken from two immature rats aged between 11 and 12 days. The testes were taken under anesthesia by ethe...
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