Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive

Temam Abrar Hamza

doi:doi:10.11648/j.ajls.20170505.11

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Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive

Temam Abrar Hamza

Published in American Journal of Life Sciences (Volume 5, Issue 5)

Received: 15 June 2017 Accepted: 23 June 2017 Published: 30 August 2017

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Abstract

Proteases are among the most important hydrolytic enzymes that found in every organism to undertake important physiological functions. They are multipurpose enzymes used in various industries such as detergent, silver recovery, food, pharmaceutical, leather, and textile industries. This work aimed to produce protease from indigenous microbes for use as detergent additive. Isolation of protease producer was undertaken using skim milk agar medium. Crude enzyme was characterized in terms of wash and stain removal tests. A total of 188 protease positive bacteria were isolated from seven samples collected from Arba Minch town. Out of 36 alkaline protease producing bacteria, one isolate designated as Bacillus sp. Cab44 was selected. The optimum activity was reached at pH 9 and 50°C. The enzyme was stable in the pH range of 7 to 10. It retained 75%, 86% and 72% activity after one hr pre-incubation at 50°C, in 15% H₂O₂ and 0.3% commercial detergent respectively. The enzyme activity was increased by Mg²⁺, Cu²⁺, and Mn²⁺, was not affected by Ca²⁺ but decreased by Zn²⁺, Hg²⁺ and Fe²⁺. It removed stains of blood and egg yolk on cotton cloth at pH 9, 40°C, 5.07 U/ml in 30-40 min. These properties suggest that protease from Bacillus sp. Cab44 could find potential application in detergent industries as good candidate of additive in detergent formulation which have economic implication.

Published in	American Journal of Life Sciences (Volume 5, Issue 5)
DOI	10.11648/j.ajls.20170505.11
Page(s)	116-124
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bacillus sp. Cab44, Detergent Additive, Enzyme, Protease

References

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APA Style

Temam Abrar Hamza. (2017). Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. American Journal of Life Sciences, 5(5), 116-124. https://doi.org/10.11648/j.ajls.20170505.11

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Temam Abrar Hamza. Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. Am. J. Life Sci. 2017, 5(5), 116-124. doi: 10.11648/j.ajls.20170505.11

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AMA Style

Temam Abrar Hamza. Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. Am J Life Sci. 2017;5(5):116-124. doi: 10.11648/j.ajls.20170505.11

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@article{10.11648/j.ajls.20170505.11,
  author = {Temam Abrar Hamza},
  title = {Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {5},
  pages = {116-124},
  doi = {10.11648/j.ajls.20170505.11},
  url = {https://doi.org/10.11648/j.ajls.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170505.11},
  abstract = {Proteases are among the most important hydrolytic enzymes that found in every organism to undertake important physiological functions. They are multipurpose enzymes used in various industries such as detergent, silver recovery, food, pharmaceutical, leather, and textile industries. This work aimed to produce protease from indigenous microbes for use as detergent additive. Isolation of protease producer was undertaken using skim milk agar medium. Crude enzyme was characterized in terms of wash and stain removal tests. A total of 188 protease positive bacteria were isolated from seven samples collected from Arba Minch town. Out of 36 alkaline protease producing bacteria, one isolate designated as Bacillus sp. Cab44 was selected. The optimum activity was reached at pH 9 and 50°C. The enzyme was stable in the pH range of 7 to 10. It retained 75%, 86% and 72% activity after one hr pre-incubation at 50°C, in 15% H2O2 and 0.3% commercial detergent respectively. The enzyme activity was increased by Mg2+, Cu2+, and Mn2+, was not affected by Ca2+ but decreased by Zn2+, Hg2+ and Fe2+. It removed stains of blood and egg yolk on cotton cloth at pH 9, 40°C, 5.07 U/ml in 30-40 min. These properties suggest that protease from Bacillus sp. Cab44 could find potential application in detergent industries as good candidate of additive in detergent formulation which have economic implication.},
 year = {2017}
}

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TY - JOUR
T1 - Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive
AU - Temam Abrar Hamza
Y1 - 2017/08/30
PY - 2017
N1 - https://doi.org/10.11648/j.ajls.20170505.11
DO - 10.11648/j.ajls.20170505.11
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 116
EP - 124
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20170505.11
AB - Proteases are among the most important hydrolytic enzymes that found in every organism to undertake important physiological functions. They are multipurpose enzymes used in various industries such as detergent, silver recovery, food, pharmaceutical, leather, and textile industries. This work aimed to produce protease from indigenous microbes for use as detergent additive. Isolation of protease producer was undertaken using skim milk agar medium. Crude enzyme was characterized in terms of wash and stain removal tests. A total of 188 protease positive bacteria were isolated from seven samples collected from Arba Minch town. Out of 36 alkaline protease producing bacteria, one isolate designated as Bacillus sp. Cab44 was selected. The optimum activity was reached at pH 9 and 50°C. The enzyme was stable in the pH range of 7 to 10. It retained 75%, 86% and 72% activity after one hr pre-incubation at 50°C, in 15% H2O2 and 0.3% commercial detergent respectively. The enzyme activity was increased by Mg2+, Cu2+, and Mn2+, was not affected by Ca2+ but decreased by Zn2+, Hg2+ and Fe2+. It removed stains of blood and egg yolk on cotton cloth at pH 9, 40°C, 5.07 U/ml in 30-40 min. These properties suggest that protease from Bacillus sp. Cab44 could find potential application in detergent industries as good candidate of additive in detergent formulation which have economic implication.
VL - 5
IS - 5
ER -

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Temam Abrar Hamza

Department of Biotechnology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia

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APA Style

Temam Abrar Hamza. (2017). Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. American Journal of Life Sciences, 5(5), 116-124. https://doi.org/10.11648/j.ajls.20170505.11

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ACS Style

Temam Abrar Hamza. Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. Am. J. Life Sci. 2017, 5(5), 116-124. doi: 10.11648/j.ajls.20170505.11

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AMA Style

Temam Abrar Hamza. Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive. Am J Life Sci. 2017;5(5):116-124. doi: 10.11648/j.ajls.20170505.11

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@article{10.11648/j.ajls.20170505.11,
  author = {Temam Abrar Hamza},
  title = {Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive},
  journal = {American Journal of Life Sciences},
  volume = {5},
  number = {5},
  pages = {116-124},
  doi = {10.11648/j.ajls.20170505.11},
  url = {https://doi.org/10.11648/j.ajls.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20170505.11},
  abstract = {Proteases are among the most important hydrolytic enzymes that found in every organism to undertake important physiological functions. They are multipurpose enzymes used in various industries such as detergent, silver recovery, food, pharmaceutical, leather, and textile industries. This work aimed to produce protease from indigenous microbes for use as detergent additive. Isolation of protease producer was undertaken using skim milk agar medium. Crude enzyme was characterized in terms of wash and stain removal tests. A total of 188 protease positive bacteria were isolated from seven samples collected from Arba Minch town. Out of 36 alkaline protease producing bacteria, one isolate designated as Bacillus sp. Cab44 was selected. The optimum activity was reached at pH 9 and 50°C. The enzyme was stable in the pH range of 7 to 10. It retained 75%, 86% and 72% activity after one hr pre-incubation at 50°C, in 15% H2O2 and 0.3% commercial detergent respectively. The enzyme activity was increased by Mg2+, Cu2+, and Mn2+, was not affected by Ca2+ but decreased by Zn2+, Hg2+ and Fe2+. It removed stains of blood and egg yolk on cotton cloth at pH 9, 40°C, 5.07 U/ml in 30-40 min. These properties suggest that protease from Bacillus sp. Cab44 could find potential application in detergent industries as good candidate of additive in detergent formulation which have economic implication.},
 year = {2017}
}

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