In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.
| Published in | American Journal of Laboratory Medicine (Volume 2, Issue 4) |
| DOI | 10.11648/j.ajlm.20170204.13 |
| Page(s) | 52-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Antisickling Activity, Antioxidant Activity, Antibacterial Activity, Inhibitory Concentration, ABTS, DPPH
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APA Style
Gédéon Bongo, Clément Inkoto, Colette Masengo, Claudine Tshiama, Emmanuel Lengbiye, et al. (2017). Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine, 2(4), 52-59. https://doi.org/10.11648/j.ajlm.20170204.13
ACS Style
Gédéon Bongo; Clément Inkoto; Colette Masengo; Claudine Tshiama; Emmanuel Lengbiye, et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Am. J. Lab. Med. 2017, 2(4), 52-59. doi: 10.11648/j.ajlm.20170204.13
AMA Style
Gédéon Bongo, Clément Inkoto, Colette Masengo, Claudine Tshiama, Emmanuel Lengbiye, et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Am J Lab Med. 2017;2(4):52-59. doi: 10.11648/j.ajlm.20170204.13
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Download@article{10.11648/j.ajlm.20170204.13,
author = {Gédéon Bongo and Clément Inkoto and Colette Masengo and Claudine Tshiama and Emmanuel Lengbiye and Ruphin Djolu and Mutwale Kapepula and Kabamba Ngombe and Théophile Mbemba and Dorothée Tshilanda and Pius Mpiana and Koto-te-Nyiwa Ngbolua},
title = {Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels},
journal = {American Journal of Laboratory Medicine},
volume = {2},
number = {4},
pages = {52-59},
doi = {10.11648/j.ajlm.20170204.13},
url = {https://doi.org/10.11648/j.ajlm.20170204.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170204.13},
abstract = {In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo.},
year = {2017}
}
TY - JOUR T1 - Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels AU - Gédéon Bongo AU - Clément Inkoto AU - Colette Masengo AU - Claudine Tshiama AU - Emmanuel Lengbiye AU - Ruphin Djolu AU - Mutwale Kapepula AU - Kabamba Ngombe AU - Théophile Mbemba AU - Dorothée Tshilanda AU - Pius Mpiana AU - Koto-te-Nyiwa Ngbolua Y1 - 2017/10/17 PY - 2017 N1 - https://doi.org/10.11648/j.ajlm.20170204.13 DO - 10.11648/j.ajlm.20170204.13 T2 - American Journal of Laboratory Medicine JF - American Journal of Laboratory Medicine JO - American Journal of Laboratory Medicine SP - 52 EP - 59 PB - Science Publishing Group SN - 2575-386X UR - https://doi.org/10.11648/j.ajlm.20170204.13 AB - In accordance with statistics from the World Health Organization, nearly 80% of populations depend on traditional medicine for primary health care. In order to scientifically validate the phyto-therapeutic wealth of the Democratic Republic of the Congo, three plants were chosen namely: Afromomum alboviolaceum, Annona senegalensis and Mondia whitei. The objective of our study was to assess the antisickling, antioxidant and antibacterial activities of the ethanolic extracts and the dichloromethane fractions of these three plants. Sickle cell blood was supplied from Centre de Médecine Mixte and Anémie SS of Yolo Sud, Kinshasa. Three bacterial strains were used including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027. The ethanolic extracts of A. alboviolaceum and A. senegalensis and dichloromethane fractions of A. alboviolaceum and M. whitei showed IC50 values < 100 μg/mL in the ABTS test. Only the dichloromethane fraction of A. senengalensis showed an IC50 value of less than 100 μg/mL for the DPPH test. The inhibitory concentration 50 (IC50) values obtained from the ABTS test are lower than those of the DPPH test. All tested extracts possess a high antisickling activity and only soluble dichloromethane extracts are active vis-à-vis Staphylococcus aureus (MIC = 31.5 μg/mL). These results constitute a scientific evidence validating the use of these three medicinal plants for the management of sickle-cell anemia in the Democratic Republic of the Congo. VL - 2 IS - 4 ER -
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