American Journal of Laboratory Medicine

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Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria

Received: Feb. 11, 2017    Accepted: Mar. 07, 2017    Published: Oct. 24, 2017
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Abstract

Cytomegalovirus cause viral congenital infection at any stage of pregnancy leading to congenital defects, which can either be primary or recurrent and hearing loss is one of the common congenital CMV infection occurring in 10-15% of infected children. This study, therefore examined the seroprevalence of cytomegalovirus among paediatric patients with hearing loss attending National Ear Care Centre Kaduna northwest Nigeria. A total of 77 samples was collected from patients aged between 0-13 years, diagnosed of hearing impairment and screened for Cytomegalovirus IgG and IgM specific antibodies using ELISA method at National Ear Care Centre Kaduna State North West Nigeria. Among the 77 (100%) patients screened, 35 (45.5%) were male, while 42 (54.5%) were female with mean age (2.04). The study reveavled that, of the total numbers screened, 62 (80.5%) were IgG specific antibodies positive, while 15 (19.5%) were IgG specific antibodies negative, and none of the patient were positive for IgM specific antibodies. the result of patients based on their gender status show male patients 21 (60%) were positive while 14 (40%) were negative for CMV IgG specific antibodies. The female is 33 (78.5%) positive and 9 (21.4%) negative for CMV IgG specific antibodies. The findings hearing impairment is associated with cytomegalosvirus showing statistically significant in IgG specific antibodies (P<0.05), while in IgM it is not statistically significant. Therefore CMV infection is prevalent among patients with Hearing impairment and affect the social economic and political status of the patients.

DOI 10.11648/j.ajlm.20170205.12
Published in American Journal of Laboratory Medicine ( Volume 2, Issue 5, September 2017 )
Page(s) 90-95
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cytomegalovirus (CMV), Hearing Loss, IgG, IgM, Congenital, Antibody

References
[1] Prescott, M. L.; Harley, J. P.; and Klein, D. A; (2005): Statement in: Microbiology. 6th Edition pp. 862.
[2] Nathalie, J. S.; Richard, W. E. (2005): Statement in: Diagnostic Procedures for Viral, Rickettsia and Chlamydial Infections 6th Edition pp. 321 –329.
[3] Boppana, S. B.; Rivera, L. B.; Fowler, K. B.; Mach, M. and Britt, W. J. (2001): Intrauterine Transmission of Cytomegalovirus to Infants of Women with Preconceptional Immunity. New England Journal of Medicine. 3334: 1366 – 1371 (s).
[4] Griffiths, P. D. and Walter, S. (2005): Cytomegalovirus. Current opinion in infectious Disease 18 (3): 242-245.
[5] Bello, C. and Whittle, H. (1991): Cytomegalovirus infection in Gambian mothers and their Babies: Journal of Clinical Pathology 1991; 44: 366-369.
[6] Timbury, C. M. (1986): Herpes Disease: In: Notes on Medical Virology 8th Edition: pp 88- 89.
[7] Fowler, K. B.; and Boppana, S. B. (2006). Congenital cytomegalovirus (CMV) infection and hearing deficit. Journal of Clinical Virology, 35 (2), 226-231.
[8] Spring, 1998: Cytomegalovirus Registry Stagno, S.; Pass, R. F.; Cloud G. Brith; W. J.; Henderson, R. F.; Walton, P. O.; Veren, D. A.; Page, F.; Alford C. A. (1986): Primary Cytomegalovirus infection in pregnancy incidence, transmission of foetus and clinical outcome.; Journal of the American Medical Association 256: 1904-1908.
[9] Gabrielli, L.; Lazzaroho, T.; Foschini, M. P.; Lanari, M.; Guerra, B.; Eusebi, V. and Poala, M. (2003): Horizontal in utero acquisition of cytomegalovirus infection in a twin pregnancy. Journal of clinical microbiology: 14 (3) 1329 – 1331 (s).
[10] Bottieau, E.; Clerinx, J.; Van den Enden, E. (2006): Infectious mononucleosis-like syndromes in febrile travellers returning from the Tropics. Journal of Travel Medicine; 13 (4): 191-197.
[11] Stagno S, Pass RF, Cloud G, Brith WJ, Henderson RF, Walton PO, Veren DA, Page F, Alford CA. Primary Cytomegalovirus infection in pregnancy incidence, transmission of foetus and clinical outcome.; Journal of the American Medical Association. 1986; 256: 1904-1908.
[12] Dworsky, M. E.; Welch, K.; Cassady, G. and Stagno, S. (1983). Occupational risk for primary cytomegalovirus infection among paediatric health-care workers. New England Journal of Medicine, 309 (16), 950–953.
[13] HO, M. (1990) Epidemiology of Cytomegalovirus infection. Reviews of Infectious Diseases; 12 (7): 5701-5710.
[14] Colugnati, A. B. F.; Stephanie, A. S. S.; Sheila, C. D. and Michael, J. C. (2007): Incidence of Cytomegalovirus infection among the general population and pregnant women in the United States. doi: 10. 1186/1471 – 2334 – 7 – 71.
[15] Ahmad, R. M.; Kawo, A. H.; Udeani, T. K. C.; Manga, S. B.; Ibrahim, M. L.; and Danjuma, B (2011): Sero-prevelence of cytomegalovirus antibodies in pregnant women attending two Hospitals in Sokoto State, North West Nigeria; Bayaro Journal of pure and applied Science, 4 (1); 63-66.
[16] Ryan, K. J. and Ray, C. G. (2004): Statement in Sherris Medical Microbiology (4th Edition) McGraw- Hill 556; 556-559.
[17] Jawetz, M. and Adelberg (2007): Statement in: Text Book Medical Microbiology 24th Edition. Pp. 441 – 445.
[18] Fowler, K. B.; Dahle, A. J.; Boppana, S. B. and Pass, R. F. (1999). New-born hearing screening: will children with hearing loss due to congenital cytomegalovirus infection be missed? Journal of Paediatrics, 135: 60-64.
[19] Morton, C. C. and Nance, W. E. (2006): New-born hearing screening-a silent revolution. New England Journal of Medicine, 354: 2151-2164.
[20] Dahle, A. J.; Fowler, K. B.; Wright, J. R.; Boppana, S. B.; Britt, W. J.; and Pass, R. F. (2000): Longitudinal investigation of hearing disorders in children with congenital cytomegalovirus. Journal of the American Academy of Audiology, 11: 283-290.
[21] Ross, D. S. (2006): Mild and Unilateral hearing loss in children. Access Audiology 5 (2) pp 6.
[22] Fowler, K. B. and Pass, R. F. (2006): Risk factors for congenital cytomegalovirus infection in the offspring of young women: Exposure to young children and recent onset of sexual activity. Paediatric; 118: 286-292.
[23] Kerry, B. T., Morrow, A., Geraghty, S., Huey, N. Sapsford, A., Schleiss, M. R. (2006): Breast milk as a source of acquisition of cytomegalovirus (HCMV) in a premature infant with sepsis syndrome: detection by real – time PCR Journal of clinical Virology; 35 (3): 33-6.
[24] Olusanya, B. O.; Luxan, L. M.; Wirz, S. L. (2005): Screening for early childhood hearing loss in Nigeria: J Med Screen; 12: 115-118.
[25] Valerie, E. N. (2006): Community Ear and Hearing Health Publications; Issue NO 3, 3: pp 1-16 The Heyford Press Ltd ISSN 1743-9914.
[26] Hollier, L. M. and Grissom, H. (2005): Human Herpes viruses in pregnancy: Cytomegalovirus, Epstein Bar virus and Varicella Zooster virus. Clinics in Perinatology; 32: 71-696.
[27] Duff, P. (2005): Immunotherapy for congenital Cytomegalovirus infection: New England Journal of Medicine; 353 (13): 1402-1404.
[28] Brooks, G. F.; Butel, J. S. and Morse, S. A. (2007): Herpes Viruses In: Jawetz, Melnick and Adelberg’s (Ed) Medical Microbiology. 24th Edition, McGraw-Hill. New York 441-445.
[29] Schleiss, M. R., (2008): Prospects for development and impact of a vaccine against congenital cytomegalovirus infection. Journal of Pediatrics; 151: 564-70.
[30] Okwori, A.; Olabode, A.; Emmuwen, E.; Lugos, M.; Okpe, E.; Okopi, J. and Adetunji, j. (2008): Sero- Epidemiological Survey of human cytomegalovirus infection among expectant mothers in Bida Nigeria. The Internet Journal of infectious Diseases; 6 (2).
[31] Grosse, S. D.; Dollard, S. C.; Ross, D. S.; (2008) Congenital cytomegalovirus (CMV) infections a cause of permanent bilateral hearing loss: A quantitative assessment. Journal of Clinical Virology. E- pub ahead of print.
[32] Lazzarotto, T., Verani, S. Guerra, B. Nicolosi, A., Lanrari, M, Landini, M. P. (2001): Prenatal indicators of cytomegalovirus infection. Journal of paediatrics; (13791): 90-5.
[33] Wang, A.; Tank, H.; Tee, C. S. (2000): Seroprevalence of Cytomegalovirus, Toxoplasmosis, and Parvovirus in pregnancy. Singapore Med J.; 41 (4): 151–155.
[34] Arabpou, M.; Kariyanee, K.; Jankhah, A.; (2007). Human cytomegalovirus in women of child bearing age throughout Fars province-Iran: A. population based cohort study. Malaysian Journal of Microbiology.; 3 (2): 23–28.
[35] Ali, S.; Askin, G.; Hakan, O; (2007): CMV seroconversion in pregnancy and the incidence of congenital CMV infection. Turk J Peadriatr.; 49: 30–36.
[36] Estripeant, D.; Moreno, Y.; Ahumada, R. (2007): Seroprevalence of Cytomegalovirus infection in peuperal women and its impact on their newborns. Ann Pediatr.; 66: 135–139.
[37] Staras SA, Dollard SC, Rayford KW, Flanders WD, Pass RF, Cannon MJ. (2006) Seroprevalence of Cytomegalosvirus infection in the United State (1988-1994). Clinical Infectious Diseases.; 43 (9): 1143- 1151.
[38] Kassim O. O.; Afolabi, O.; Ako-Nai, K. A.; Torimiro, S. E. A.; Littleton, G. K.; Oke, O. O.; Grisson, F. C.; (1987): Cytomegalovirus antibodies in breast milk and sera of mother- infant pairs. J Trop Pediatr; 33 (2): 75-77.
[39] Akinsegun, A. A.; Kabiru, A. R.; Adeniyi, A. A.; Kikelomo, O. W. (2011) Sero prevalence of Cytomegalovirus antibodies amongst normal pregnant women in Nigeria. Int. J Women Health 2011; 3: 423-428.
[40] Edward, Deborah S., Edward, Isaac U., Nwankiti, O. Shallangwa, Ishaku B., Abdullahi, Musa M., (2015): The Sero-Prevalence of Cytomegalovirus (IgM) Antibodies among Pregnant Women Attending Ante-natal Clinic at the General Hospital Kafanchan, Kaduna State Nigeria. British Microbiology Research Journal; 2015, Vol 9 (5) pp 1-6, 2015. Article no. BMRJ. 16863 ISSN: 2231-0886. DOI: 10.9734/BMRJ/2015/16863.
[41] Adisa, T. Bukbuk, D. Harry, T. (2010): Maternofoetal transfer of Cytomegalovirus IgG antibodies in Maiduguri, North Eastern Nigeria. The Internet Journal of Microbiology. Volume 9 Number 1. DOI: 10.5580/c53.
[42] Redwan, A. N. Ahmed, M. M. AlAwfi, M. S. (2011) Prevalence Study of Cytomegalovirus infection among foreign man power in Jeddah Saudi Arabia; African Journal Of Microbiology Research Vol 5 (17) pp 2539-2549.
[43] Yeroh, M. Aminu, M. Musa, B. O. (2014): Seroprevalence of Cytomegalovirus infection among Pregnant Women in Kaduna Nigeria. African Journal of Clinical and Experimental Microbiology Vol 16 (1) 37-44.
[44] Barbi M., Binda, S., Caroppa, S., Amborestti, U., Corbetta, C., Sergi, P. (2003). A wider role for congenital cytomegalovirus infection in sensorineural hearing loss. Pediatric Infectious Disease Journal, 22 (1), 39–42.
[45] Ogawa, H., Suzutani, T., Baba, Y., Koyano, S., Nozawa, N., Ishibashi, K., (2007) Etiology of severe sensorineural hearing loss in children: independent impact of congenital cytomegalovirus infection and GJB2 mutations. Journal of Infectious Diseases, 195 (6), 782–8.
[46] Center for Disease Control (CDC) USA, Principles of Epidemiology in Public Health Practice Third Edition (2012).
Cite This Article
  • APA Style

    Edward Isaac Usman, Elayo Salihu, Ogboi Johnbull Sunny, Musa Abdullahi Maikano, Edward Deborah Shetu, et al. (2017). Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria. American Journal of Laboratory Medicine, 2(5), 90-95. https://doi.org/10.11648/j.ajlm.20170205.12

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    ACS Style

    Edward Isaac Usman; Elayo Salihu; Ogboi Johnbull Sunny; Musa Abdullahi Maikano; Edward Deborah Shetu, et al. Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria. Am. J. Lab. Med. 2017, 2(5), 90-95. doi: 10.11648/j.ajlm.20170205.12

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    AMA Style

    Edward Isaac Usman, Elayo Salihu, Ogboi Johnbull Sunny, Musa Abdullahi Maikano, Edward Deborah Shetu, et al. Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria. Am J Lab Med. 2017;2(5):90-95. doi: 10.11648/j.ajlm.20170205.12

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  • @article{10.11648/j.ajlm.20170205.12,
      author = {Edward Isaac Usman and Elayo Salihu and Ogboi Johnbull Sunny and Musa Abdullahi Maikano and Edward Deborah Shetu and Shallangwa Bata Ishaku and Meshubi Florence and Isaac Agyigra},
      title = {Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria},
      journal = {American Journal of Laboratory Medicine},
      volume = {2},
      number = {5},
      pages = {90-95},
      doi = {10.11648/j.ajlm.20170205.12},
      url = {https://doi.org/10.11648/j.ajlm.20170205.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajlm.20170205.12},
      abstract = {Cytomegalovirus cause viral congenital infection at any stage of pregnancy leading to congenital defects, which can either be primary or recurrent and hearing loss is one of the common congenital CMV infection occurring in 10-15% of infected children. This study, therefore examined the seroprevalence of cytomegalovirus among paediatric patients with hearing loss attending National Ear Care Centre Kaduna northwest Nigeria. A total of 77 samples was collected from patients aged between 0-13 years, diagnosed of hearing impairment and screened for Cytomegalovirus IgG and IgM specific antibodies using ELISA method at National Ear Care Centre Kaduna State North West Nigeria. Among the 77 (100%) patients screened, 35 (45.5%) were male, while 42 (54.5%) were female with mean age (2.04). The study reveavled that, of the total numbers screened, 62 (80.5%) were IgG specific antibodies positive, while 15 (19.5%) were IgG specific antibodies negative, and none of the patient were positive for IgM specific antibodies. the result of patients based on their gender status show male patients 21 (60%) were positive while 14 (40%) were negative for CMV IgG specific antibodies. The female is 33 (78.5%) positive and 9 (21.4%) negative for CMV IgG specific antibodies. The findings hearing impairment is associated with cytomegalosvirus showing statistically significant in IgG specific antibodies (P<0.05), while in IgM it is not statistically significant. Therefore CMV infection is prevalent among patients with Hearing impairment and affect the social economic and political status of the patients.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria
    AU  - Edward Isaac Usman
    AU  - Elayo Salihu
    AU  - Ogboi Johnbull Sunny
    AU  - Musa Abdullahi Maikano
    AU  - Edward Deborah Shetu
    AU  - Shallangwa Bata Ishaku
    AU  - Meshubi Florence
    AU  - Isaac Agyigra
    Y1  - 2017/10/24
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    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
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    EP  - 95
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20170205.12
    AB  - Cytomegalovirus cause viral congenital infection at any stage of pregnancy leading to congenital defects, which can either be primary or recurrent and hearing loss is one of the common congenital CMV infection occurring in 10-15% of infected children. This study, therefore examined the seroprevalence of cytomegalovirus among paediatric patients with hearing loss attending National Ear Care Centre Kaduna northwest Nigeria. A total of 77 samples was collected from patients aged between 0-13 years, diagnosed of hearing impairment and screened for Cytomegalovirus IgG and IgM specific antibodies using ELISA method at National Ear Care Centre Kaduna State North West Nigeria. Among the 77 (100%) patients screened, 35 (45.5%) were male, while 42 (54.5%) were female with mean age (2.04). The study reveavled that, of the total numbers screened, 62 (80.5%) were IgG specific antibodies positive, while 15 (19.5%) were IgG specific antibodies negative, and none of the patient were positive for IgM specific antibodies. the result of patients based on their gender status show male patients 21 (60%) were positive while 14 (40%) were negative for CMV IgG specific antibodies. The female is 33 (78.5%) positive and 9 (21.4%) negative for CMV IgG specific antibodies. The findings hearing impairment is associated with cytomegalosvirus showing statistically significant in IgG specific antibodies (P<0.05), while in IgM it is not statistically significant. Therefore CMV infection is prevalent among patients with Hearing impairment and affect the social economic and political status of the patients.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Department of Medical Laboratory, Medical Microbiology Laboratory, Federal Neuropsychiatric Hospital, Barnawa, Nigeria

  • Department of Virology, Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria

  • Research Department Jedima International Health Consult Ltd, Lagos, Nigeria

  • Department of Medical Laboratory, Medical Microbiology Laboratory, Federal Neuropsychiatric Hospital, Barnawa, Nigeria

  • Department of Virology, Tehilah Medical Laboratory, Sabon Tasha, Kaduna State, Nigeria

  • Department of Animal Health, Federal College of Animal Health and Production Technology, Vom, Plateau State, Nigeria

  • Department of Medical Laboratory, Medical Microbiology Laboratory, Federal Neuropsychiatric Hospital, Barnawa, Nigeria

  • Department of Medical Laboratory, Microbiology Unit, National Ear Care Cente, Kaduna State, Nigeria

  • Section