Determination of Antimicrobial Resistance of <i>Enterobacteriaceae</i> in Patients with Community Acquired Urinary Tract Infections

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir

doi:doi:10.11648/j.ajlm.20170206.20

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Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir

Published in American Journal of Laboratory Medicine (Volume 2, Issue 6)

Received: 2 November 2017 Accepted: 14 November 2017 Published: 4 January 2018

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Abstract

This study was conducted in Port Sudan city, Red Sea state during the period from January to June 2017 to investigate antimicrobial resistance of Enterobacteriaceae isolated from patients suffering from community acquired urinary tract infections. One hundred and fifty urine specimens were collected from patients attended Port Sudan Teaching Hospital. The specimens were cultured on blood agar and Mac Conkey's agar and Cystine Lysine Electrolyte deficient (CLED) agar for primary isolation of pathogens. Identification of the isolates was done by colonial morphology, Gram stain and Routine biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of Enterobacteriaceae to Imipenem, Ciprofloxacin, Chloramphenicol, Amikacin, Piperacillin, Tetracycline, Ceftazidime and Ticarcillin. Out one hundred and fifty urine specimens examined Enterobacteriaceae was detected in only 49(32.6%) specimens. The results revealed that the antimicrobial resistance of Enterobacteriaceae was as follows: Imipenem (6.1%), Ciprofloxacin (32.6%), Chloramphenicol (48.9%), Amikacin (61.2%), Piperacillin (79.5%), Tetracycline (83.6%), Ceftazidime (89.7%) and Ticarcillin (91.8%). Females were more affected than males (60%) and young adults were more affected than other age groups. Imipenem represented the least sensitive antimicrobial agent (6.1%), while Ticarcillin showed the highest resistance rate.

Published in	American Journal of Laboratory Medicine (Volume 2, Issue 6)
DOI	10.11648/j.ajlm.20170206.20
Page(s)	179-182
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Enterobacteriaceae, Urinary Tract Infections, CLED, Port Sudan

References

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[2]	Bush K, (2008). Extended-spectrum β-lactamases in North America. Clin Microbiol Infect 14, 134–143.
[3]	Sakoulas G and Moellering R, (2008). Increasing Antibiotic Resistance among Methicillin-resistant Staphylococcus aureus strains. Clin Infect Dis. 46: S360–S367.
[4]	Johann D and Kevin B (2008). Extended-spectrum β-lactamase-producing Enterobacteriaceae:an emerging public-health concern. The Lancet Infectious Disease, Volume 8, Issue 3, pp 159-166.
[5]	Mahrouki Achour N, Chouchan C, Moussa M, and Belhadj O, (2008). Identification of Plasmid-encoded Extended Spectrum β-lactamases Produced by a clinical Strain of Proteus mirabilis. Pathologie Biologie. Volume 57, Issue3, pp 55-59.
[6]	George G, Mel D, Kim N, Aleksandra W, Patricia J, James A, Philippe L, Andrew W, Michael R, and Daryi J, (2008). Antimicrobial Susceptibility of 3931 Organisms Isolated from Intensive Care Units in canada. Diagnostic Microbiology and Infectious Disease. Volume 62, Pages 67-80.
[7]	Mims Cedric, dockrell. Hazel. M, Goering. V, Roitt. Ivan, Wakelin. Derek and Zuckerman. Mark. (2004). Medical microbiology, third edition, ELSEVIER MOSBY.
[8]	Tsai L, Clifford M, Yih R, Pei C, Hui Y, Jui F, Monto H and TSAR Participating Hospitals, (2003). The Status of Antimicrobial Resistance in Taiwan among Gram-negative Pathogens: the Taiwan Surveillance of Antimicrobial Resistance (TSAR) Program. Diagnostic Microbiology and Infectious Disease. Volume 48, Issue 3, pp 211-219.
[9]	Sita N, Merode T, Bartelds A, and Stobberingh E, (2005), Antibiotic Treatment and Resistance of Unselected Uropathogens in the Elderly. International Journal of Antimicrobial Agent. Volume27. Issue3, pp 236-241.
[10]	Nijssen, Florijn A, Bonten M, Schmitz F, Verhoef J and Fluit A, (2004). Beta-lactam Susceptibilities and Prevalence of ESBL-producing Isolates among more than 5000 European Enterobacteriaceae isolates, University Medical Center Utrecht. International Journal of Antimicrobial Agent. Volume24. Issue6, pp 585-591.
[11]	Farmer J, 1999. Enterobacteriaceae, pp.442-458. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. ASM Press, Washington, D. C.

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APA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. (2018). Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. American Journal of Laboratory Medicine, 2(6), 179-182. https://doi.org/10.11648/j.ajlm.20170206.20

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ACS Style

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir. Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. Am. J. Lab. Med. 2018, 2(6), 179-182. doi: 10.11648/j.ajlm.20170206.20

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AMA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. Am J Lab Med. 2018;2(6):179-182. doi: 10.11648/j.ajlm.20170206.20

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@article{10.11648/j.ajlm.20170206.20,
  author = {Abd Elrahman Mustafa Abd Elrahman Osman and Shingray Osman Hashim and Mohammed Abdall Musa and Omer Mohammed Tahir},
  title = {Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections},
  journal = {American Journal of Laboratory Medicine},
  volume = {2},
  number = {6},
  pages = {179-182},
  doi = {10.11648/j.ajlm.20170206.20},
  url = {https://doi.org/10.11648/j.ajlm.20170206.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170206.20},
  abstract = {This study was conducted in Port Sudan city, Red Sea state during the period from January to June 2017 to investigate antimicrobial resistance of Enterobacteriaceae isolated from patients suffering from community acquired urinary tract infections. One hundred and fifty urine specimens were collected from patients attended Port Sudan Teaching Hospital. The specimens were cultured on blood agar and Mac Conkey's agar and Cystine Lysine Electrolyte deficient (CLED) agar for primary isolation of pathogens. Identification of the isolates was done by colonial morphology, Gram stain and Routine biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of Enterobacteriaceae to Imipenem, Ciprofloxacin, Chloramphenicol, Amikacin, Piperacillin, Tetracycline, Ceftazidime and Ticarcillin. Out one hundred and fifty urine specimens examined Enterobacteriaceae was detected in only 49(32.6%) specimens. The results revealed that the antimicrobial resistance of Enterobacteriaceae was as follows: Imipenem (6.1%), Ciprofloxacin (32.6%), Chloramphenicol (48.9%), Amikacin (61.2%), Piperacillin (79.5%), Tetracycline (83.6%), Ceftazidime (89.7%) and Ticarcillin (91.8%). Females were more affected than males (60%) and young adults were more affected than other age groups. Imipenem represented the least sensitive antimicrobial agent (6.1%), while Ticarcillin showed the highest resistance rate.},
 year = {2018}
}

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TY  - JOUR
T1  - Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections
AU  - Abd Elrahman Mustafa Abd Elrahman Osman
AU  - Shingray Osman Hashim
AU  - Mohammed Abdall Musa
AU  - Omer Mohammed Tahir
Y1  - 2018/01/04
PY  - 2018
N1  - https://doi.org/10.11648/j.ajlm.20170206.20
DO  - 10.11648/j.ajlm.20170206.20
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 179
EP  - 182
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20170206.20
AB  - This study was conducted in Port Sudan city, Red Sea state during the period from January to June 2017 to investigate antimicrobial resistance of Enterobacteriaceae isolated from patients suffering from community acquired urinary tract infections. One hundred and fifty urine specimens were collected from patients attended Port Sudan Teaching Hospital. The specimens were cultured on blood agar and Mac Conkey's agar and Cystine Lysine Electrolyte deficient (CLED) agar for primary isolation of pathogens. Identification of the isolates was done by colonial morphology, Gram stain and Routine biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of Enterobacteriaceae to Imipenem, Ciprofloxacin, Chloramphenicol, Amikacin, Piperacillin, Tetracycline, Ceftazidime and Ticarcillin. Out one hundred and fifty urine specimens examined Enterobacteriaceae was detected in only 49(32.6%) specimens. The results revealed that the antimicrobial resistance of Enterobacteriaceae was as follows: Imipenem (6.1%), Ciprofloxacin (32.6%), Chloramphenicol (48.9%), Amikacin (61.2%), Piperacillin (79.5%), Tetracycline (83.6%), Ceftazidime (89.7%) and Ticarcillin (91.8%). Females were more affected than males (60%) and young adults were more affected than other age groups. Imipenem represented the least sensitive antimicrobial agent (6.1%), while Ticarcillin showed the highest resistance rate.
VL  - 2
IS  - 6
ER  -

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Author Information

Abd Elrahman Mustafa Abd Elrahman Osman

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Shingray Osman Hashim

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Mohammed Abdall Musa

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Omer Mohammed Tahir

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan

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APA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. (2018). Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. American Journal of Laboratory Medicine, 2(6), 179-182. https://doi.org/10.11648/j.ajlm.20170206.20

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ACS Style

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir. Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. Am. J. Lab. Med. 2018, 2(6), 179-182. doi: 10.11648/j.ajlm.20170206.20

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AMA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections. Am J Lab Med. 2018;2(6):179-182. doi: 10.11648/j.ajlm.20170206.20

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@article{10.11648/j.ajlm.20170206.20,
  author = {Abd Elrahman Mustafa Abd Elrahman Osman and Shingray Osman Hashim and Mohammed Abdall Musa and Omer Mohammed Tahir},
  title = {Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections},
  journal = {American Journal of Laboratory Medicine},
  volume = {2},
  number = {6},
  pages = {179-182},
  doi = {10.11648/j.ajlm.20170206.20},
  url = {https://doi.org/10.11648/j.ajlm.20170206.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170206.20},
  abstract = {This study was conducted in Port Sudan city, Red Sea state during the period from January to June 2017 to investigate antimicrobial resistance of Enterobacteriaceae isolated from patients suffering from community acquired urinary tract infections. One hundred and fifty urine specimens were collected from patients attended Port Sudan Teaching Hospital. The specimens were cultured on blood agar and Mac Conkey's agar and Cystine Lysine Electrolyte deficient (CLED) agar for primary isolation of pathogens. Identification of the isolates was done by colonial morphology, Gram stain and Routine biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of Enterobacteriaceae to Imipenem, Ciprofloxacin, Chloramphenicol, Amikacin, Piperacillin, Tetracycline, Ceftazidime and Ticarcillin. Out one hundred and fifty urine specimens examined Enterobacteriaceae was detected in only 49(32.6%) specimens. The results revealed that the antimicrobial resistance of Enterobacteriaceae was as follows: Imipenem (6.1%), Ciprofloxacin (32.6%), Chloramphenicol (48.9%), Amikacin (61.2%), Piperacillin (79.5%), Tetracycline (83.6%), Ceftazidime (89.7%) and Ticarcillin (91.8%). Females were more affected than males (60%) and young adults were more affected than other age groups. Imipenem represented the least sensitive antimicrobial agent (6.1%), while Ticarcillin showed the highest resistance rate.},
 year = {2018}
}

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TY  - JOUR
T1  - Determination of Antimicrobial Resistance of Enterobacteriaceae in Patients with Community Acquired Urinary Tract Infections
AU  - Abd Elrahman Mustafa Abd Elrahman Osman
AU  - Shingray Osman Hashim
AU  - Mohammed Abdall Musa
AU  - Omer Mohammed Tahir
Y1  - 2018/01/04
PY  - 2018
N1  - https://doi.org/10.11648/j.ajlm.20170206.20
DO  - 10.11648/j.ajlm.20170206.20
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 179
EP  - 182
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20170206.20
AB  - This study was conducted in Port Sudan city, Red Sea state during the period from January to June 2017 to investigate antimicrobial resistance of Enterobacteriaceae isolated from patients suffering from community acquired urinary tract infections. One hundred and fifty urine specimens were collected from patients attended Port Sudan Teaching Hospital. The specimens were cultured on blood agar and Mac Conkey's agar and Cystine Lysine Electrolyte deficient (CLED) agar for primary isolation of pathogens. Identification of the isolates was done by colonial morphology, Gram stain and Routine biochemical tests. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of Enterobacteriaceae to Imipenem, Ciprofloxacin, Chloramphenicol, Amikacin, Piperacillin, Tetracycline, Ceftazidime and Ticarcillin. Out one hundred and fifty urine specimens examined Enterobacteriaceae was detected in only 49(32.6%) specimens. The results revealed that the antimicrobial resistance of Enterobacteriaceae was as follows: Imipenem (6.1%), Ciprofloxacin (32.6%), Chloramphenicol (48.9%), Amikacin (61.2%), Piperacillin (79.5%), Tetracycline (83.6%), Ceftazidime (89.7%) and Ticarcillin (91.8%). Females were more affected than males (60%) and young adults were more affected than other age groups. Imipenem represented the least sensitive antimicrobial agent (6.1%), while Ticarcillin showed the highest resistance rate.
VL  - 2
IS  - 6
ER  -

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