International Journal of Microbiology and Biotechnology

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New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans

Received: Mar. 05, 2019    Accepted: Apr. 08, 2019    Published: May 15, 2019
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Abstract

Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.

DOI 10.11648/j.ijmb.20190401.14
Published in International Journal of Microbiology and Biotechnology ( Volume 4, Issue 1, March 2019 )
Page(s) 19-23
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Candida albicans, C. dubliniensis, Chlamydospore Production, Differential Medium, Seeds, Leaves

References
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[13] KIRKPATRICK, W. R.; REVANKAR, S. G.; McATEE, R. K. et al. - Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates. J. Clin. Microbiol. 1998. 36: 3007-3012.
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    Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. (2019). New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. International Journal of Microbiology and Biotechnology, 4(1), 19-23. https://doi.org/10.11648/j.ijmb.20190401.14

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    ACS Style

    Zenab Abduhall Krema; Ebtesam Salem Mokthar; Hameda Sadkali El Magrahi; Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int. J. Microbiol. Biotechnol. 2019, 4(1), 19-23. doi: 10.11648/j.ijmb.20190401.14

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    AMA Style

    Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int J Microbiol Biotechnol. 2019;4(1):19-23. doi: 10.11648/j.ijmb.20190401.14

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  • @article{10.11648/j.ijmb.20190401.14,
      author = {Zenab Abduhall Krema and Ebtesam Salem Mokthar and Hameda Sadkali El Magrahi and Mohamed Suliman Ellabib},
      title = {New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {4},
      number = {1},
      pages = {19-23},
      doi = {10.11648/j.ijmb.20190401.14},
      url = {https://doi.org/10.11648/j.ijmb.20190401.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijmb.20190401.14},
      abstract = {Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans
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    AU  - Ebtesam Salem Mokthar
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    JO  - International Journal of Microbiology and Biotechnology
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    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20190401.14
    AB  - Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.
    VL  - 4
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    ER  - 

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Author Information
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya

  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya

  • Department of Medical Laboratory Science, Faculty of Medical Technology, Tripoli, Libya

  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya

  • Section