Background and Objective: Huaizhi Mixture is widely used in clinical practice for the treatment of hemorrhoids, but its pharmacological mechanism remains unclear. This study systematically analyzed the potential active components, targets, and signaling pathways of Huaizhi Mixture in treating hemorrhoids based on network pharmacology and molecular docking technology, aiming to elucidate its pharmacological mechanism. Methods: The potential targets of Huaizhi Mixture were obtained from the TCMSP database, while hemorrhoid-related targets were collected from GeneCards and the NCBI databases. The overlapping targets between the drug and disease were then identified through Venn diagram analysis. Cytoscape 3.8.0 was employed to construct a "drug-component-target-disease" network, and the STRING database was used to build a protein-protein interaction (PPI) network. Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID platform. Finally, molecular docking technology was applied to validate the binding affinity between key active components and core targets. Results: A total of 381 potential active components of Huaizhi Mixture were screened, with quercetin, luteolin, ursolic acid, and apigenin identified as the key components. Forty-two intersection targets between the drug and disease were identified, including core targets such as IL6, IL1B, TNF, and AKT1. GO analysis revealed that the targets were primarily enriched in biological processes such as cellular response to lipopolysaccharides, cell proliferation, and apoptosis regulation. KEGG analysis indicated that the relevant pathways involved the IL-17, HIF-1, TNF, and relaxin signaling pathways. Molecular docking confirmed strong binding activity between the four key components and the core targets. Conclusion: Huaizhi Mixture may exert its anti-hemorrhoidal effects through a synergistic mechanism involving multiple components, targets, and pathways, providing new insights for the study of hemorrhoid mechanisms and drug development.

Huaizhi Mixture, Hemorrhoids, Traditional Chinese Medicine Compound, Network Pharmacology, Molecular Docking Technology, Signaling Pathways