Because of the rising costs of chemical fertilizers and the growing environmental concerns, there is a need in the use of bio-inoculants in crop production and soil fertility enhancement. A field study was therefore conducted at Jimma zone Kersa and Nadi gibe District during 2019/2020 and 2020/2021 main cropping season to evaluate soybean nodulating rhizobia isolates under field condition in order to select the best performing rhizobia strain for nodulation, yield and yield attributes of soybean. Eight treatments were arranged in RCBD with three replications. Nodule number, number of pods per plant, dry biomass and grain yield was responded significantly to the interaction effects of Tgp-3 inoculation. The maximum mean yield of 2506.5kg per hectare and mean dry biomass of 8.12 ton per hectare was obtained from Tgp-3 inoculation. Similarly the highest mean nodule number of 298.68 per plant was counted after inoculation with Tgp-3. Similarly the highest mean number of pods per plant was obtained by inoculation of Tgp-3. These results demonstrated that the dominance of Tgp-3 rhizobium isolate and could explain the effectiveness of the isolate in South west soil of Ethiopia. Inoculation of Tgp-3 isolate with Clark 63-K soybean variety was being considered effective symbionts for Clark 63-K soybean production in the area.
| Published in | Advances (Volume 4, Issue 1) |
| DOI | 10.11648/j.advances.20230401.12 |
| Page(s) | 9-16 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Effective Rhizobia, Growth, Nodulation, Soybean, Isolate
| [1] | Mekuanint Lewoyehu, Zinash Alemu & Enyew Adgo (2020). The effects of land management on soil fertility and nutrient balance in Kecha and Laguna micro watersheds, Amhara Region, Northwestern, Ethiopia, Cogent Food & Agriculture, 6:1, 1853996, DOI: 10.1080/23311932.2020.1853996 |
| [2] | Karim MR, Islam F, Akkas Ali M, Haque F. (2001). On-farm trail with Rhizobium inoculants on lentil. Bangladesh J Agric Res 26, 93-94. |
| [3] | Okazaki, S., Nukui, N., Sugawara, M. and Minamisawa, K. (2004). Rhizobial strategies to enhance symbiotic Interactions: Rhizobiotoxine and 1-Aminocyclopropane-1-Carboxylate deaminase. Microb. Environ. 19 (2): 99-111. |
| [4] | Ellafi AM, Gadalla A, Galal YGM. (2011). Biofertilizers in action: contributions of BNF in sustainable agricultural ecosystems. Int. Sci.Res. J. 3 (2): 108-116. |
| [5] | FAO, (2016). Promoting regional trade in pulses in the Horn of Africa. |
| [6] | Nedumaran, S., Abinaya, P., Jyosthnaa, P., Shraavya, B., Parthasarathy, Rao and Cynthia Bantilan. (2015). Grain Legumes Production, Consumption and Trade Trends in Developing Countries. Working Paper Series No. 60. ICRISAT Research Program, Markets, Institutions and Policies. Patancheru 502 324, Telangana, India: International Crops Research Institute for the Semi-Arid Tropics. pp: 64. |
| [7] | Central Statistical Authority (CSA) (2000). Statistical abstract of Ethiopia. Addis Ababa, Ethiopia: Central Statistical Authority. |
| [8] | Martin, J. H. and Leonard, W. H. (1967). Soybeans. In: Principles of Field Crop Production, pp. 643^662. MacMillan, New York. |
| [9] | Hirth, F. (1908). The ancient History of China and the End of the Cou Dynasty. Columbia University Press, New York. |
| [10] | CSA. (2012). Agricultural sample survey 2011-2012. In Annual Bulletin, CSA, Addis Ababa, Ethiopia. |
| [11] | Urgessa Tilahun Bekabil 2015. Empirical review of production, productivity and marketability of soya bean in Ethiopia. Ijunesst, 8, 61–66. 10.14257/ijunesst. |
| [12] | Bala, A., Karanja, N., Murwira, M., Lwimbi, L., Abaidoo, R., Giller, K. (2011). Production and use of Rhizobial inoculants in Africa, www.N2Africa.org, pp: 21. |
| [13] | Asfaw Hailemariam and Angaw Tsige (2006). Biological Nitrogen Fixation Research in Food Legumes in Ethiopia. Proceedings of a National Workshop on Food and Forage legumes, September, 2003. Addis Ababa, Ethiopia. |
| [14] | Crozat Y, Cleyet-Marel JC, Giraud JJ, Obaton M. (1982). Survival rates of Rhizobium japonicum populations introduced into soils. Soil Biol Biochem 14, 401-405. |
| [15] | Jensen ES, Peoples MB, Boddey RM, Gresshoff PM, Hauggaard- Nielsen H, Alves BJR, Morrison MJ (2012). Legumes for Mitigation of Climate Change and Provision of Feedstocks for Biofuels and Biorefineries. Agron. Sustain. Dev. 32: 329-364. |
| [16] | Jefwa J, Pypers P, Jemo M, Thuita M, Mutegi E, Laditi M, Faye A, Kavoo A, Munyahali W, Herrmann L. (2014). Do commercial biological and chemical products increase crop yields and economic returns under smallholder farmer conditions? In Challenges and Opportunities for Agricultural Intensification of the Humid Highland Systems of subSaharan Africa, eds. Vanlauwe B, VanAsten PG. pp. 81–96. Springer International Publishing, Cham, Switzerland. |
| [17] | Daniel Muleta, Maarten H. Ryder & Matthew D. Denton (2017). The potential for rhizobial inoculation to increase soybean grain yields on acid soils in Ethiopia, Soil Science and Plant Nutrition, 63:5, 441-451, DOI: 10.1080/00380768.2017.1370961 |
| [18] | Vincent, JM (1970). A manual for practical study of root nodule bacteria. IBP Handbook No. 15, Blackwell Scientific Publishers, Oxford, 164p. |
| [19] | FAO-Unesco. (1974). FAO/UNESCO Soil Map of the World 1: 5,000,000, UNESCO, Paris. |
| [20] | Philpotts H. (1976). Filter mud as a carrier for Rhizobium inoculants. J. Appl. Bacteriol., 41, 277–281. 10.1111/j.1365-2672.1976.tb00631.x |
| [21] | Somasegaran P, Hoben H. J. (1985). Methods in legume-Rhizobium technology. Hawaii Institute of Tropical Agriculture and Human Resources. p. 550. |
| [22] | Deaker R, Roughley RJ, Kennedy IR. (2004). Legume seed Inoculation Technology. A Review. Soil Biol. Biochem. 36: 1275-1288. |
| [23] | Gee GW, Bauder JW. (1986). Particle-size analysis. In Methods of Soil Analysis. Part 1. Physical and Mineralogical Methods, ed. Klute A, pp. 383–411. American Society of Agronomy, Inc. and Soil Science Society of America, Inc., Madison, WI. |
| [24] | Van Reeuwijk L. (2002). Procedures for Soil Analysis, International soil reference and information centre (ISRIC), Wageningen, The Netherlands. |
| [25] | Sahlemedhin Sertsu S, Bekele T eds (2000). Procedures for Soil and Plant Analysis, National Soil Reseach Center EARO, Addis Ababa, Ethiopia. |
| [26] | Purcino, H. M. A., Festin, P. M. and Elkan, G. H. (2000). Identification of Effective Strains of Bradyrhizobium for Archis pintoi. Trop. Agric., 77: 226-231. |
| [27] | Gomez KA, Gomez AA. (1984). Statistical Procedures for Agricultural Research. 2nd edition. John Wiley and Sons, Inc. pp. 84-118. |
| [28] | London, J. R. (Ed.), (1991). Booker Tropical Soil Manual: A Handbook for Soil Survey and Agricultural Land Eval uation in the Tropics and Subtropics. Longman. |
| [29] | FAO. (1990). Guideline for Soil Description. Rome, Italy, pp: 193. |
| [30] | Nyalemegbe, K., Oteng and Asuming-Brempong, J. (2009). Integrated organic-inorganic fertilizer management for rice production on the vertisols of the Accra Plains of Ghana, W. Afric. J. App. Ecol., 16, 23-33. |
| [31] | Mungai, N. W. and Karubiu, N. M. (2012). Effectiveness of rhizobia isolates from Njoro soils (Kenya) and commercial inoculants in nodulation of common beans (Phaseolus vulgaris). Journal of Agricultural Science and Technology, pp: 47-59. |
| [32] | Tahir, M. M., Abbasi, M. K. Rahim, N. Khaliq A and Kazmi, M. H. (2009). Effects of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. Afr. J. Biotech. 8, 6191-6200. |
| [33] | Kutcher, H. R., Lafond, G, Johnston, A. M, Miller, P. R., Gill, K. S., May, W. E., Hogg, T., Johnson, E., Biederbeck V. O., and Nybo, B. (2002). Rhizobium inoculant and seed-applied fungicide effects on field pea production. Canadian Journal of Plant Science, 82: 645-651. |
| [34] | Assefa Keneni, Fassil Assefa and Prabu, P. C. (2010). Characterization of Acid and Salt Tolerant Rhizobial Strains Isolated from Faba Bean Fields of Wollo, Northern Ethiopia. Journal of Agricultural Science and Technology, 2: 365-376. |
| [35] | Dubey, S. K., (1998). Response of soybean to biofertilizers with and without nitrogen, phosphorus and potassium on swell-shrink soil. Ind. J. Agron. 43, 546-549. |
| [36] | Guo, Y., Ni Y. and Huang, J. (2010). Effects of rhizobium, arbuscular mycorrhiza and lime on nodulation, growth and nutrient uptake of Lucerne in acid purplish soil in China. Tropical Grasslands, 44: 109-114. |
| [37] | Xavier, L. J. C. and Germida, J. J. (2003). Selective interactions between arbuscular mycorrhizal fungi and Rhizobium leguminosarum bv. Viceae enhance pea yield and nutrition. Biology and Fertility of Soils, 37: 261-267. |
| [38] | Raposeiras, R., Marriel, I. E., Muzzi, M. R. S., Paiva, E., Pereira Filho, I. A., Carvalhais, L. C. and Sá, N. M. H. D. (2006). Rhizobium Strains Competitiveness on Bean Nodulation in Cerrado Soils. Pesquisa Agropecuária Brasileira, 41, 439-447. http://dx.doi.org/10.1590/S0100-204X2006000300010 |
| [39] | Howieson, J. G., Ewing, M. A. & D'Antuono, M. F. (1988). Selection for acid tolerance inRhizobium meliloti. Plant Soil 105, 179–188.https://doi.org/10.1007/BF02376781 |
APA Style
Reshid Abafita Abawari, Gabresilase Hailu. (2023). Collection, Isolation and Symbiotic Efficiency Test and Field Evaluation of Native Rhizobia for Soybean (G. max) in Acid-Prone Areas of South-West Ethiopia. Advances, 4(1), 9-16. https://doi.org/10.11648/j.advances.20230401.12
ACS Style
Reshid Abafita Abawari; Gabresilase Hailu. Collection, Isolation and Symbiotic Efficiency Test and Field Evaluation of Native Rhizobia for Soybean (G. max) in Acid-Prone Areas of South-West Ethiopia. Advances. 2023, 4(1), 9-16. doi: 10.11648/j.advances.20230401.12
AMA Style
Reshid Abafita Abawari, Gabresilase Hailu. Collection, Isolation and Symbiotic Efficiency Test and Field Evaluation of Native Rhizobia for Soybean (G. max) in Acid-Prone Areas of South-West Ethiopia. Advances. 2023;4(1):9-16. doi: 10.11648/j.advances.20230401.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.advances.20230401.12,
author = {Reshid Abafita Abawari and Gabresilase Hailu},
title = {Collection, Isolation and Symbiotic Efficiency Test and Field Evaluation of Native Rhizobia for Soybean (G. max) in Acid-Prone Areas of South-West Ethiopia},
journal = {Advances},
volume = {4},
number = {1},
pages = {9-16},
doi = {10.11648/j.advances.20230401.12},
url = {https://doi.org/10.11648/j.advances.20230401.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.advances.20230401.12},
abstract = {Because of the rising costs of chemical fertilizers and the growing environmental concerns, there is a need in the use of bio-inoculants in crop production and soil fertility enhancement. A field study was therefore conducted at Jimma zone Kersa and Nadi gibe District during 2019/2020 and 2020/2021 main cropping season to evaluate soybean nodulating rhizobia isolates under field condition in order to select the best performing rhizobia strain for nodulation, yield and yield attributes of soybean. Eight treatments were arranged in RCBD with three replications. Nodule number, number of pods per plant, dry biomass and grain yield was responded significantly to the interaction effects of Tgp-3 inoculation. The maximum mean yield of 2506.5kg per hectare and mean dry biomass of 8.12 ton per hectare was obtained from Tgp-3 inoculation. Similarly the highest mean nodule number of 298.68 per plant was counted after inoculation with Tgp-3. Similarly the highest mean number of pods per plant was obtained by inoculation of Tgp-3. These results demonstrated that the dominance of Tgp-3 rhizobium isolate and could explain the effectiveness of the isolate in South west soil of Ethiopia. Inoculation of Tgp-3 isolate with Clark 63-K soybean variety was being considered effective symbionts for Clark 63-K soybean production in the area.},
year = {2023}
}
TY - JOUR T1 - Collection, Isolation and Symbiotic Efficiency Test and Field Evaluation of Native Rhizobia for Soybean (G. max) in Acid-Prone Areas of South-West Ethiopia AU - Reshid Abafita Abawari AU - Gabresilase Hailu Y1 - 2023/02/21 PY - 2023 N1 - https://doi.org/10.11648/j.advances.20230401.12 DO - 10.11648/j.advances.20230401.12 T2 - Advances JF - Advances JO - Advances SP - 9 EP - 16 PB - Science Publishing Group SN - 2994-7200 UR - https://doi.org/10.11648/j.advances.20230401.12 AB - Because of the rising costs of chemical fertilizers and the growing environmental concerns, there is a need in the use of bio-inoculants in crop production and soil fertility enhancement. A field study was therefore conducted at Jimma zone Kersa and Nadi gibe District during 2019/2020 and 2020/2021 main cropping season to evaluate soybean nodulating rhizobia isolates under field condition in order to select the best performing rhizobia strain for nodulation, yield and yield attributes of soybean. Eight treatments were arranged in RCBD with three replications. Nodule number, number of pods per plant, dry biomass and grain yield was responded significantly to the interaction effects of Tgp-3 inoculation. The maximum mean yield of 2506.5kg per hectare and mean dry biomass of 8.12 ton per hectare was obtained from Tgp-3 inoculation. Similarly the highest mean nodule number of 298.68 per plant was counted after inoculation with Tgp-3. Similarly the highest mean number of pods per plant was obtained by inoculation of Tgp-3. These results demonstrated that the dominance of Tgp-3 rhizobium isolate and could explain the effectiveness of the isolate in South west soil of Ethiopia. Inoculation of Tgp-3 isolate with Clark 63-K soybean variety was being considered effective symbionts for Clark 63-K soybean production in the area. VL - 4 IS - 1 ER -
Information
Email: