Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect

Li Huiduan; Yu Jianzhong

doi:doi:10.11648/j.ajcbe.20170101.12

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Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect

Li Huiduan, Yu Jianzhong

Published in American Journal of Chemical and Biochemical Engineering (Volume 1, Issue 1)

Received: 8 September 2016 Accepted: 7 December 2016 Published: 7 January 2017

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Abstract

Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 1, Issue 1)
DOI	10.11648/j.ajcbe.20170101.12
Page(s)	7-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Extract Methodology, Total Flavonoids, Ginger, Extraction Ratio, Response Surface Methodology, Hydroxyl Radicals Scavenging Effect

References

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[7]	Wang ZC, Jiang YR, Liu XW, et al. Response Surface Methodology Optimization of total flavonids from ginger leaf and study on antioxidation effect[J]. Natural product research and developmen, 2015, 27(9): 1582-1588.
[8]	Mo KJ, Cheng C. Huang P. et al. Study on extract technology, purification and structural appraisal of flavonoid from ginger [J]. Food science, 2005, 26(9): 229-233.
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APA Style

Li Huiduan, Yu Jianzhong. (2017). Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. American Journal of Chemical and Biochemical Engineering, 1(1), 7-16. https://doi.org/10.11648/j.ajcbe.20170101.12

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ACS Style

Li Huiduan; Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am. J. Chem. Biochem. Eng. 2017, 1(1), 7-16. doi: 10.11648/j.ajcbe.20170101.12

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AMA Style

Li Huiduan, Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am J Chem Biochem Eng. 2017;1(1):7-16. doi: 10.11648/j.ajcbe.20170101.12

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@article{10.11648/j.ajcbe.20170101.12,
  author = {Li Huiduan and Yu Jianzhong},
  title = {Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {1},
  number = {1},
  pages = {7-16},
  doi = {10.11648/j.ajcbe.20170101.12},
  url = {https://doi.org/10.11648/j.ajcbe.20170101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20170101.12},
  abstract = {Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.},
 year = {2017}
}

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TY  - JOUR
T1  - Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect
AU  - Li Huiduan
AU  - Yu Jianzhong
Y1  - 2017/01/07
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcbe.20170101.12
DO  - 10.11648/j.ajcbe.20170101.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 7
EP  - 16
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20170101.12
AB  - Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.
VL  - 1
IS  - 1
ER  -

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Author Information

Li Huiduan

Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China
Yu Jianzhong

Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China

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APA Style

Li Huiduan, Yu Jianzhong. (2017). Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. American Journal of Chemical and Biochemical Engineering, 1(1), 7-16. https://doi.org/10.11648/j.ajcbe.20170101.12

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ACS Style

Li Huiduan; Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am. J. Chem. Biochem. Eng. 2017, 1(1), 7-16. doi: 10.11648/j.ajcbe.20170101.12

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AMA Style

Li Huiduan, Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am J Chem Biochem Eng. 2017;1(1):7-16. doi: 10.11648/j.ajcbe.20170101.12

Copy | Download

@article{10.11648/j.ajcbe.20170101.12,
  author = {Li Huiduan and Yu Jianzhong},
  title = {Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {1},
  number = {1},
  pages = {7-16},
  doi = {10.11648/j.ajcbe.20170101.12},
  url = {https://doi.org/10.11648/j.ajcbe.20170101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20170101.12},
  abstract = {Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect
AU  - Li Huiduan
AU  - Yu Jianzhong
Y1  - 2017/01/07
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcbe.20170101.12
DO  - 10.11648/j.ajcbe.20170101.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 7
EP  - 16
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20170101.12
AB  - Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.
VL  - 1
IS  - 1
ER  -

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