Antibacterial Effects of Chemical Constituents Isolated from the Roots of <i>Cordia myxa</i> (Boraginaceae) on <i>Salmonella typhi</i> (Enterobacteriaceae)

Dabole Bernard; Matcheme Mathieu; Moussa Djaouda; Abdou Tchoukoua; Wakayansam Bouba Romeo; Meli Alain; Loura Benoit

doi:doi:10.11648/j.ajcbe.20210501.11

| Peer-Reviewed

Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)

Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, Meli Alain, Loura Benoit

Published in American Journal of Chemical and Biochemical Engineering (Volume 5, Issue 1)

Received: 22 January 2021 Accepted: 30 January 2021 Published: 10 February 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 5, Issue 1)
DOI	10.11648/j.ajcbe.20210501.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Cordia myxa, Antibacterial Effect, Salmonella typhi, Triterpene

References

[1]	Bolou. G. EK, Attioua. B, N’guessam. A. C., Coulibaly A, N’Guessan J. D. et Djaman A. J., «In vitro evaluation of antibacterial activity of Terminalia glaucescens planch extract on Salmonella typhi and Salmonella typhimurium.», Newsletter of Liege Sciences royal Society, 2011, vol. 80, P. 772-790.
[2]	Mariani-Kurkdjian. P, Bingen. E, Gault. G, Jourdan Da Silva N, Weill FX, “Escherichia coli O104: H4 south-west France, June 2011”, Lancet Infectious Diseases, 2011, Vol. 11 (10),. P. 732-733.
[3]	Nataro. J. P, Kaper, “Diarrheagenic Escherichia coli”, Clim Microbiol Rev, 1999, Vol. 11, P. 142-201.
[4]	Kaper. J. B, Nataro. J. P, Mobley H. L, “Pathologenic Escherichia coli”, Nat Rev Microbiol., 2004, Vol. 2. P. 123-40.
[5]	Haddouchi. F, Zerhouni. K, Sidi-Yekhelef. A., «Evaluation of antimicrobial activity of various extraits of Helichrysum stoechas», Newsletter of Liege Sciences royal Society, 2016, Vol. 85,. P. 152-159.
[6]	Chaouche. T. M, Haddouchi. F, Ksouri. R, “In vitro evaluation of antioxidant activity of the hydro-methanolic extracts of junipeur oxycedrus subsp. Oxycedrus”, Phytothérapie, 2013, Vol. 11 (4), P. 244-249.
[7]	Sabira. B, Sobiya. P, Bina. SS, Shazia. K, Shahina. F, Musarrat R., “Chemical constituents of Cordia latifolia and Their Nematicidal Activity”, Chemistry and Biodiversity, 2011, Vol. 8, P. 850-861.
[8]	Thamer. M. J, Suhad. F., Ibrahim. S, Aljubory, Qabas. N. L,”Phytochemical study and antibacterial activity of crude alkalo¨ds and mucilage of Cordia myxa in Iraq”, International Journal of Pharmaceutical Sciences Review and Research, 2016, Vol. 39 (1), P. 232-236.
[9]	Arbonnier. M, «shrubs and lianas of ouest african dried zoneArbustes et lianes des zone », CIRAD MNHN, Paris, 2002.
[10]	Bauer. A. W, Kirby. W. M. M, Sherris. T. C, Truck. M, “Antibiotical susceptibility testing by a standardized single disc method”, American journal of clinical pathology, Vol. 45, 1966, P. 493-496.
[11]	Barry. A. L, Thornsberry. C,” Susceptibility test, diffusion test procedure”, American journal of clinical pathology, 1985, Vol. 19, P. 492-500.
[12]	FSM, French Society of Microbiology, Recommendations of Antibiogram committee of French Society of Microbiology, 2008, P. 49.
[13]	Adesokan. A. A, Akanji M. A, “Antibacterial potentials of aqueous extract of Enantia chlorantha stem barck”, African journal of Biotechnology, 2007, P. 2502-2505.
[14]	Doughari. J. H, Pukuma. M. S, De. N, “Antibacterial effects of blanites aegyptiaca L. Drel and Moringa oleifera Lam. On Salmonella typhi”, African journal of biotechnology, 2007, Vol.. 6 (19), P. 2212-2215.
[15]	Gandini Chloe, «Phytochemical study of a peruvian medicinal plant Cordia lutea Lam. Which demonstrated a biological activity against Helicobacter pylori bacteria», Faculty of Pharmaceutical Sciences, University of Toulouse III Paul Sabatier, 2016, P. 32-45.
[16]	Goad L. John and Toshihiro Akihisa, “Analysis of sterols”, Blackie Academic & Professional 1997, P. 4-23.
[17]	Kumar. U. P, Sreedhar. S, Purushothaman. E,” Secondary metabolites from the heart Wood of Combretum albidum G. Don”, International journal of Psychophysiology, 2015, P. 319-324.
[18]	Mahato. SB, Kundu AP,” ¹³C NMR Spectra of pentacyclic triterpenoid: A compilation and some salient features”, Phytochemistry, 1994, Vol. 37, P. 1547-1575.
[19]	Gracez. F. R, Gracez. W. S, Santana. ALBD, Alves. M. M, Matos MFC, Scaliante AB. “Bioactive flavonoids and triterpenes from Terminalia fagifolia (Combretaceae)”, J Braz. Chem. Soc., 2006, Vol. 17, P. 1223-1228.
[20]	FSM/EUCAST, (Antibiogram committee of French Society of Microbiology), European Committee on Antimicrobial Susceptibility Testing (CA-SFM/EUCAST), 2016, p117.
[21]	Li. Z. H, Cai. M, Liu. Y. S, Sun. P. L, Luo. S. L, Antibacterial activity and mecanisms of essential oil from Citru medica L. var. sarcodactylis. Molecules, 2019, Vol. 24, P. 1577.
[22]	Xiao-Nian. X, Fang. W, Yi-Ting. Y, Jing. L, Yue-Zhen. L, Xing. Y, Antibacterial activity and Mode of action of Dihydromyricetin from Ampelopsis. Molecules, 2019, Vol. 24 (15), P. 2831.
[23]	Ben Abdallah. R, Frikha. D, Maalej. S, Sassi. S, In vitro evaluation of the antibacterial and anti fungal activities of marine algae. Le Journal de l’information Médicale de Sfax, Nº 31, 2019, P. 38-44.

Cite This Article

Plain Text BibTeX RIS

APA Style

Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. (2021). Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). American Journal of Chemical and Biochemical Engineering, 5(1), 1-7. https://doi.org/10.11648/j.ajcbe.20210501.11

Copy | Download

ACS Style

Dabole Bernard; Matcheme Mathieu; Moussa Djaouda; Abdou Tchoukoua; Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am. J. Chem. Biochem. Eng. 2021, 5(1), 1-7. doi: 10.11648/j.ajcbe.20210501.11

Copy | Download

AMA Style

Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am J Chem Biochem Eng. 2021;5(1):1-7. doi: 10.11648/j.ajcbe.20210501.11

Copy | Download

@article{10.11648/j.ajcbe.20210501.11,
  author = {Dabole Bernard and Matcheme Mathieu and Moussa Djaouda and Abdou Tchoukoua and Wakayansam Bouba Romeo and Meli Alain and Loura Benoit},
  title = {Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajcbe.20210501.11},
  url = {https://doi.org/10.11648/j.ajcbe.20210501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20210501.11},
  abstract = {Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.},
 year = {2021}
}

Copy | Download

TY - JOUR
T1 - Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)
AU - Dabole Bernard
AU - Matcheme Mathieu
AU - Moussa Djaouda
AU - Abdou Tchoukoua
AU - Wakayansam Bouba Romeo
AU - Meli Alain
AU - Loura Benoit
Y1 - 2021/02/10
PY - 2021
N1 - https://doi.org/10.11648/j.ajcbe.20210501.11
DO - 10.11648/j.ajcbe.20210501.11
T2 - American Journal of Chemical and Biochemical Engineering
JF - American Journal of Chemical and Biochemical Engineering
JO - American Journal of Chemical and Biochemical Engineering
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2639-9989
UR - https://doi.org/10.11648/j.ajcbe.20210501.11
AB - Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.
VL - 5
IS - 1
ER -

Copy | Download

Author Information

Dabole Bernard

Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon
Matcheme Mathieu

Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon
Moussa Djaouda

Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon
Abdou Tchoukoua

Department of Chemistry, Faculty of science, University of Maroua, Maroua, Cameroon
Wakayansam Bouba Romeo

Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua, Cameroon
Meli Alain

Department of Chemistry, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon
Loura Benoit

Department of Life and Earth Sciences, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. (2021). Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). American Journal of Chemical and Biochemical Engineering, 5(1), 1-7. https://doi.org/10.11648/j.ajcbe.20210501.11

Copy | Download

ACS Style

Dabole Bernard; Matcheme Mathieu; Moussa Djaouda; Abdou Tchoukoua; Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am. J. Chem. Biochem. Eng. 2021, 5(1), 1-7. doi: 10.11648/j.ajcbe.20210501.11

Copy | Download

AMA Style

Dabole Bernard, Matcheme Mathieu, Moussa Djaouda, Abdou Tchoukoua, Wakayansam Bouba Romeo, et al. Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae). Am J Chem Biochem Eng. 2021;5(1):1-7. doi: 10.11648/j.ajcbe.20210501.11

Copy | Download

@article{10.11648/j.ajcbe.20210501.11,
  author = {Dabole Bernard and Matcheme Mathieu and Moussa Djaouda and Abdou Tchoukoua and Wakayansam Bouba Romeo and Meli Alain and Loura Benoit},
  title = {Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajcbe.20210501.11},
  url = {https://doi.org/10.11648/j.ajcbe.20210501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20210501.11},
  abstract = {Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.},
 year = {2021}
}

Copy | Download