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Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae

Received: 30 August 2024     Accepted: 18 September 2024     Published: 29 September 2024
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Abstract

Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.

Published in American Journal of Chemical and Biochemical Engineering (Volume 8, Issue 2)
DOI 10.11648/j.ajcbe.20240802.12
Page(s) 45-57
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bioethanol, Fruit Peels, Pretreatment, Hydrolysis, Fermentation, Saccharomyces cerevisiae, Optimization

References
[1] Vijayanand, C., Kamaraj, S., Karthikeyan, S., & Sriramajayam, S. (2016). Research Article characterization of indigenous biomass. 8(50), 2124–2127.
[2] Tsegu, G., Birri, D. J., Tigu, F., & Tesfaye, A. (2022). Bioethanol production from biodegradable wastes using native yeast isolates from Ethiopian traditional alcoholic beverages. Biocatalysis and Agricultural Biotechnology, 43, 102401.
[3] Jahid, M., Gupta, A., & Sharma, D. K. (2018). Biotechniques Production of Bioethanol from Fruit Wastes (Banana, Papaya, Pineapple and Mango Peels) Under Milder Conditions. 8(3).
[4] Singh, A., & Bishnoi, N. R. (2013). Comparative study of various pretreatment techniques for ethanol production from water hyacinth. Industrial Crops and Products, 44, 283–289.
[5] Salam, N. (2018). Review of ethanol production from fruit wastes. Procedings of Research World International Conference, June, 1–5.
[6] Pavithra, C. S., Devi, S. S., Suneetha, J. W., & Durga Rani, C. V. (2017). Nutritional properties of papaya peel. The Pharma Innovation Journal NAAS Rating TPI, 6(7), 170173.
[7] Mitiku, A. A., & Hatsa, T. M. (2020). Bioethanol production from decaying fruits peel using Saccharomyces cerevisiae. International Journal of Current Research and Academic Review, 8(5), 50–59.
[8] Maisyarah, A., Shiun, J., Nasir, F., & Hashim, H. (2019). Characteristics of Cellulose, Hemicellulose and Lignin of MD2 Pineapple Biomass. 7(December 2018), 79–84.
[9] Kabenge, I., Omulo, G., Banadda, N., Seay, J., Zziwa, A., & Kiggundu, N. (2018). Characterization of Banana Peels Wastes as Potential Slow Pyrolysis Feedstock. Journal of Sustainable Development, 11(2), 14.
[10] Hu, J., Arantes, V., & Saddler, J. N. (2011). The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: Is it an additive or synergistic effect? Biotechnology for Biofuels, 4(October).
[11] Guragain, Y. N., De Coninck, J., Husson, F., Durand, A., & Rakshit, S. K. (2011). Comparison of some new pretreatment methods for second generation bioethanol production from wheat straw and water hyacinth. Bioresource Technology, 102(6), 4416–4424.
[12] Faustine, A. S., & Djamaan, A. (2021). Bioethanol Production from Various Agricultural Waste Substrate using Saccharomyces cerevisiae. 16(1), 7–13.
[13] Debebe, D., Gabriel, T., Brhane, Y., Temesgen, A., Nigatu, M., & Marew, T. (2018). Comparative in Vitro Evaluation of Brands of Clotrimazole Cream Formulations Marketed in Ethiopia. Journal of Drug Delivery and Therapeutics, 8(1), 17–22.
[14] Danmaliki, G. I., Muhammad, A. M., Shamsuddeen, A. A., & Usman, B. J. (2016). Bioethanol Production from Banana Peels Bioethanol Production from Banana Peels. June.
[15] Barrera, E., & Cajero, P. (2014). com Characterization of Lignocellulosic Fruit Waste as an Alternative Feedstock for Bioethanol Production. 9(2), 1873–1885.
[16] Ayeni, A. O., Adeeyo, O. A., Oresegun, O. M., & Oladimeji, T. E. (2015). Compositional analysis of lignocellulosic materials: Evaluation of an economically viable method suitable for woody and non-woody biomass. American Journal of Engineering Research, 44, 2320–2847.
[17] Amanullah, A., & Kapilan, R. (2021). Utilization of bioethanol generated from papaw peel waste for hand sanitizer production. 8(Iii), 101–124.
[18] Abdulla, R., Derman, E., Ravintaran, P. T., & Azmah, S. (2017). Fuel Ethanol Production from Papaya Waste using Immobilized Saccharomyces cerevisiae. 2, 112–123.
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  • APA Style

    Tadesse, A. A. (2024). Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. American Journal of Chemical and Biochemical Engineering, 8(2), 45-57. https://doi.org/10.11648/j.ajcbe.20240802.12

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    ACS Style

    Tadesse, A. A. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am. J. Chem. Biochem. Eng. 2024, 8(2), 45-57. doi: 10.11648/j.ajcbe.20240802.12

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    AMA Style

    Tadesse AA. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am J Chem Biochem Eng. 2024;8(2):45-57. doi: 10.11648/j.ajcbe.20240802.12

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  • @article{10.11648/j.ajcbe.20240802.12,
      author = {Asab Alemneh Tadesse},
      title = {Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae
    },
      journal = {American Journal of Chemical and Biochemical Engineering},
      volume = {8},
      number = {2},
      pages = {45-57},
      doi = {10.11648/j.ajcbe.20240802.12},
      url = {https://doi.org/10.11648/j.ajcbe.20240802.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20240802.12},
      abstract = {Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae
    
    AU  - Asab Alemneh Tadesse
    Y1  - 2024/09/29
    PY  - 2024
    N1  - https://doi.org/10.11648/j.ajcbe.20240802.12
    DO  - 10.11648/j.ajcbe.20240802.12
    T2  - American Journal of Chemical and Biochemical Engineering
    JF  - American Journal of Chemical and Biochemical Engineering
    JO  - American Journal of Chemical and Biochemical Engineering
    SP  - 45
    EP  - 57
    PB  - Science Publishing Group
    SN  - 2639-9989
    UR  - https://doi.org/10.11648/j.ajcbe.20240802.12
    AB  - Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
    
    VL  - 8
    IS  - 2
    ER  - 

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