Cloning and Expression Analysis of <i>GmCYP78A5</i> Promoter

Xiaofeng Chen; Qiuli Du; Chunmei Zhao; Zhaoyong Lv; Ren-Gao Xue

doi:doi:10.11648/j.ajpb.20190401.12

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Cloning and Expression Analysis of GmCYP78A5 Promoter

Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue

Published in American Journal of Plant Biology (Volume 4, Issue 1)

Received: 21 April 2019 Accepted: 17 June 2019 Published: 4 July 2019

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Abstract

CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.

Published in	American Journal of Plant Biology (Volume 4, Issue 1)
DOI	10.11648/j.ajpb.20190401.12
Page(s)	7-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Soybean, GmCYP78A5 Promoter, Tissue Specific Expression, GUS Assay

References

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[3]	Eriksson S, Stransfeld L, Adamski N M, Breuninger H, Lenhard M (2010). Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Curr Biol, 20 (6):527-32.
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[6]	Adamski N M, Anastasiou E, Eriksson S, O'Neill C M, and Lenhard M (2009). Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Proc. Natl. Acad. Sci. USA, 106 (47): 20115-20120.
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[8]	Wei J, Mao W H., Lin Y J, and Chen H (2012). Isolation and characterization of a novel rice constitutive promoter. Journal of Huazhong Agricultural University, 31 (2): 139-146.
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APA Style

Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. (2019). Cloning and Expression Analysis of GmCYP78A5 Promoter. American Journal of Plant Biology, 4(1), 7-11. https://doi.org/10.11648/j.ajpb.20190401.12

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ACS Style

Xiaofeng Chen; Qiuli Du; Chunmei Zhao; Zhaoyong Lv; Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am. J. Plant Biol. 2019, 4(1), 7-11. doi: 10.11648/j.ajpb.20190401.12

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AMA Style

Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am J Plant Biol. 2019;4(1):7-11. doi: 10.11648/j.ajpb.20190401.12

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@article{10.11648/j.ajpb.20190401.12,
  author = {Xiaofeng Chen and Qiuli Du and Chunmei Zhao and Zhaoyong Lv and Ren-Gao Xue},
  title = {Cloning and Expression Analysis of GmCYP78A5 Promoter},
  journal = {American Journal of Plant Biology},
  volume = {4},
  number = {1},
  pages = {7-11},
  doi = {10.11648/j.ajpb.20190401.12},
  url = {https://doi.org/10.11648/j.ajpb.20190401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20190401.12},
  abstract = {CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.},
 year = {2019}
}

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TY  - JOUR
T1  - Cloning and Expression Analysis of GmCYP78A5 Promoter
AU  - Xiaofeng Chen
AU  - Qiuli Du
AU  - Chunmei Zhao
AU  - Zhaoyong Lv
AU  - Ren-Gao Xue
Y1  - 2019/07/04
PY  - 2019
N1  - https://doi.org/10.11648/j.ajpb.20190401.12
DO  - 10.11648/j.ajpb.20190401.12
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 7
EP  - 11
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20190401.12
AB  - CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.
VL  - 4
IS  - 1
ER  -

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Author Information

Xiaofeng Chen

College of Life Sciences, Qingdao Agricultural University, Qingdao, China
Qiuli Du

Quancheng College, University of Jinan, Penglai, China
Chunmei Zhao

College of Life Sciences, Qingdao Agricultural University, Qingdao, China
Zhaoyong Lv

College of Life Sciences, Qingdao Agricultural University, Qingdao, China
Ren-Gao Xue

College of Life Sciences, Qingdao Agricultural University, Qingdao, China

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Plain Text BibTeX RIS

APA Style

Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. (2019). Cloning and Expression Analysis of GmCYP78A5 Promoter. American Journal of Plant Biology, 4(1), 7-11. https://doi.org/10.11648/j.ajpb.20190401.12

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ACS Style

Xiaofeng Chen; Qiuli Du; Chunmei Zhao; Zhaoyong Lv; Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am. J. Plant Biol. 2019, 4(1), 7-11. doi: 10.11648/j.ajpb.20190401.12

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AMA Style

Xiaofeng Chen, Qiuli Du, Chunmei Zhao, Zhaoyong Lv, Ren-Gao Xue. Cloning and Expression Analysis of GmCYP78A5 Promoter. Am J Plant Biol. 2019;4(1):7-11. doi: 10.11648/j.ajpb.20190401.12

Copy | Download

@article{10.11648/j.ajpb.20190401.12,
  author = {Xiaofeng Chen and Qiuli Du and Chunmei Zhao and Zhaoyong Lv and Ren-Gao Xue},
  title = {Cloning and Expression Analysis of GmCYP78A5 Promoter},
  journal = {American Journal of Plant Biology},
  volume = {4},
  number = {1},
  pages = {7-11},
  doi = {10.11648/j.ajpb.20190401.12},
  url = {https://doi.org/10.11648/j.ajpb.20190401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20190401.12},
  abstract = {CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Cloning and Expression Analysis of GmCYP78A5 Promoter
AU  - Xiaofeng Chen
AU  - Qiuli Du
AU  - Chunmei Zhao
AU  - Zhaoyong Lv
AU  - Ren-Gao Xue
Y1  - 2019/07/04
PY  - 2019
N1  - https://doi.org/10.11648/j.ajpb.20190401.12
DO  - 10.11648/j.ajpb.20190401.12
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 7
EP  - 11
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.20190401.12
AB  - CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.
VL  - 4
IS  - 1
ER  -

Copy | Download