Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P < 0.05) reduced the elevated levels of Serum bilirubin, SGPT, SGOT and SALP compared to the CCl4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.
| Published in | Biochemistry and Molecular Biology (Volume 1, Issue 2) |
| DOI | 10.11648/j.bmb.20160102.12 |
| Page(s) | 17-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Antioxidant Activity, Carbon Tetrachloride, Ethanolic Extracts, Hepatoprotective Activity
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APA Style
Khondakar Mahmudul Hasan, Samina Yesmin, Sarkar Farhana Akhter, Subrata Paul, Sabarni Sarker, et al. (2016). Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochemistry and Molecular Biology, 1(2), 17-22. https://doi.org/10.11648/j.bmb.20160102.12
ACS Style
Khondakar Mahmudul Hasan; Samina Yesmin; Sarkar Farhana Akhter; Subrata Paul; Sabarni Sarker, et al. Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochem. Mol. Biol. 2016, 1(2), 17-22. doi: 10.11648/j.bmb.20160102.12
AMA Style
Khondakar Mahmudul Hasan, Samina Yesmin, Sarkar Farhana Akhter, Subrata Paul, Sabarni Sarker, et al. Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats. Biochem Mol Biol. 2016;1(2):17-22. doi: 10.11648/j.bmb.20160102.12
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Download@article{10.11648/j.bmb.20160102.12,
author = {Khondakar Mahmudul Hasan and Samina Yesmin and Sarkar Farhana Akhter and Subrata Paul and Sabarni Sarker and Md Ariful Islam and Mir Imam Ibne Wahed and Md Rafiqul Islam Khan},
title = {Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats},
journal = {Biochemistry and Molecular Biology},
volume = {1},
number = {2},
pages = {17-22},
doi = {10.11648/j.bmb.20160102.12},
url = {https://doi.org/10.11648/j.bmb.20160102.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160102.12},
abstract = {Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P 4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity.},
year = {2016}
}
TY - JOUR T1 - Hepatoprotective Potentiality of Various Fractions of Ethanolic Extracts of Lawsonia Inermis (Henna) Leaves Against Chemical-Induced Hepatitis in Rats AU - Khondakar Mahmudul Hasan AU - Samina Yesmin AU - Sarkar Farhana Akhter AU - Subrata Paul AU - Sabarni Sarker AU - Md Ariful Islam AU - Mir Imam Ibne Wahed AU - Md Rafiqul Islam Khan Y1 - 2016/08/21 PY - 2016 N1 - https://doi.org/10.11648/j.bmb.20160102.12 DO - 10.11648/j.bmb.20160102.12 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 17 EP - 22 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20160102.12 AB - Liver is a metabolically active organ responsible for many vital life functions. More than 900 drugs, toxins, and herbs have been reported to cause liver injury. In spite of tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease. In the present study, we investigated the hepatoprotective activity of ethanolic extracts of various fractions (Ethyl acetate, Petroleum ether and Chloroform) of Lowsonia inermis leaves in carbon tetrachloride (CCl4)-induced hepatitis rats. Hepatotoxicity was induced in rats by intraperitoneal injection of CCl4 on alternate days for 7 days. After 7 days of pretreatment of test extracts, the biochemical markers such as Serum bilirubin, Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP), were examined. The extracts at the doses of 200 mg/kg p.o. significantly (P 4-treated group alone. The findings of the study indicate that the leaf extract of L. inermis showed a potential hepatoprotective activity and the protective action might be manifested by reducing the oxidative stress as the leaf extracts possess flavonoid and phenolic compounds which have free radical scavenging and antioxidant activity. However, further study is necessary uncovering the exact molecular mechanism of hepatoprotective activity. VL - 1 IS - 2 ER -
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