Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria

Oyetola Moradeke Toyosi; Agarry Olubunmi; Oyetola Simeon

doi:doi:10.11648/j.bmb.20170206.11

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Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria

Oyetola Moradeke Toyosi, Agarry Olubunmi, Oyetola Simeon

Published in Biochemistry and Molecular Biology (Volume 2, Issue 6)

Received: 8 September 2017 Accepted: 23 September 2017 Published: 5 November 2017

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Abstract

This study was undertaken to screen for Lactic Acid Bacteria (LAB) from indigenous fermented seeds; dawadawa. Isolation of LAB was carried out on MRS Agar. Biochemical, morphological and physiological examinations according to the Bergeys manual were used to identify the bacterial isolates. Six bacterial strains were obtained and given the designations DN, DO, DJ, DT, DG and DH respectively. The obtained colonies were all capable of fermenting lactose, sucrose, trehalose, glucose, maltose and lactulose as the sole carbon source in the broth. Morphological examination revealed that the isolates obtained were non-spore forming gram positive colonies ranging from creamy to white, clear, cocci and rod shaped, smooth textured and flat elevation with transparent opacity. Their growth profile study revealed that all isolates appeared to be mesophilc with progressive growth patterns observed between 24-96 hours at the same parameters for incubation. Isolates DO and DJ were observed to exhibit the fastest growth as determined by measuring optical density of the cells in broth using spectrophotometer at 550nm. Conclusions from the observed experiment indicate that isolated LABs from dawadawa could be applied in other probiotics as starter cultures and be improved for other commercial uses.

Published in	Biochemistry and Molecular Biology (Volume 2, Issue 6)
DOI	10.11648/j.bmb.20170206.11
Page(s)	67-72
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Lactic Acid Bacteria, Growth Profile, pH, Salinity

References

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APA Style

Oyetola Moradeke Toyosi, Agarry Olubunmi, Oyetola Simeon. (2017). Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochemistry and Molecular Biology, 2(6), 67-72. https://doi.org/10.11648/j.bmb.20170206.11

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ACS Style

Oyetola Moradeke Toyosi; Agarry Olubunmi; Oyetola Simeon. Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochem. Mol. Biol. 2017, 2(6), 67-72. doi: 10.11648/j.bmb.20170206.11

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AMA Style

Oyetola Moradeke Toyosi, Agarry Olubunmi, Oyetola Simeon. Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochem Mol Biol. 2017;2(6):67-72. doi: 10.11648/j.bmb.20170206.11

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@article{10.11648/j.bmb.20170206.11,
  author = {Oyetola Moradeke Toyosi and Agarry Olubunmi and Oyetola Simeon},
  title = {Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {67-72},
  doi = {10.11648/j.bmb.20170206.11},
  url = {https://doi.org/10.11648/j.bmb.20170206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.11},
  abstract = {This study was undertaken to screen for Lactic Acid Bacteria (LAB) from indigenous fermented seeds; dawadawa. Isolation of LAB was carried out on MRS Agar. Biochemical, morphological and physiological examinations according to the Bergeys manual were used to identify the bacterial isolates. Six bacterial strains were obtained and given the designations DN, DO, DJ, DT, DG and DH respectively. The obtained colonies were all capable of fermenting lactose, sucrose, trehalose, glucose, maltose and lactulose as the sole carbon source in the broth. Morphological examination revealed that the isolates obtained were non-spore forming gram positive colonies ranging from creamy to white, clear, cocci and rod shaped, smooth textured and flat elevation with transparent opacity. Their growth profile study revealed that all isolates appeared to be mesophilc with progressive growth patterns observed between 24-96 hours at the same parameters for incubation. Isolates DO and DJ were observed to exhibit the fastest growth as determined by measuring optical density of the cells in broth using spectrophotometer at 550nm. Conclusions from the observed experiment indicate that isolated LABs from dawadawa could be applied in other probiotics as starter cultures and be improved for other commercial uses.},
 year = {2017}
}

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TY  - JOUR
T1  - Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria
AU  - Oyetola Moradeke Toyosi
AU  - Agarry Olubunmi
AU  - Oyetola Simeon
Y1  - 2017/11/05
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.11
DO  - 10.11648/j.bmb.20170206.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 67
EP  - 72
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.11
AB  - This study was undertaken to screen for Lactic Acid Bacteria (LAB) from indigenous fermented seeds; dawadawa. Isolation of LAB was carried out on MRS Agar. Biochemical, morphological and physiological examinations according to the Bergeys manual were used to identify the bacterial isolates. Six bacterial strains were obtained and given the designations DN, DO, DJ, DT, DG and DH respectively. The obtained colonies were all capable of fermenting lactose, sucrose, trehalose, glucose, maltose and lactulose as the sole carbon source in the broth. Morphological examination revealed that the isolates obtained were non-spore forming gram positive colonies ranging from creamy to white, clear, cocci and rod shaped, smooth textured and flat elevation with transparent opacity. Their growth profile study revealed that all isolates appeared to be mesophilc with progressive growth patterns observed between 24-96 hours at the same parameters for incubation. Isolates DO and DJ were observed to exhibit the fastest growth as determined by measuring optical density of the cells in broth using spectrophotometer at 550nm. Conclusions from the observed experiment indicate that isolated LABs from dawadawa could be applied in other probiotics as starter cultures and be improved for other commercial uses.
VL  - 2
IS  - 6
ER  -

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Author Information

Oyetola Moradeke Toyosi

Department of Health and Biomedical Sciences, Federal Ministry of Science and Technology, Abuja, Nigeria
Agarry Olubunmi

Department of Microbiology, University of Abuja, Abuja, Nigeria
Oyetola Simeon

Department of Animal Science, University of Abuja, Abuja, Nigeria

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Plain Text BibTeX RIS

APA Style

Oyetola Moradeke Toyosi, Agarry Olubunmi, Oyetola Simeon. (2017). Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochemistry and Molecular Biology, 2(6), 67-72. https://doi.org/10.11648/j.bmb.20170206.11

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ACS Style

Oyetola Moradeke Toyosi; Agarry Olubunmi; Oyetola Simeon. Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochem. Mol. Biol. 2017, 2(6), 67-72. doi: 10.11648/j.bmb.20170206.11

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AMA Style

Oyetola Moradeke Toyosi, Agarry Olubunmi, Oyetola Simeon. Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria. Biochem Mol Biol. 2017;2(6):67-72. doi: 10.11648/j.bmb.20170206.11

Copy | Download

@article{10.11648/j.bmb.20170206.11,
  author = {Oyetola Moradeke Toyosi and Agarry Olubunmi and Oyetola Simeon},
  title = {Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria},
  journal = {Biochemistry and Molecular Biology},
  volume = {2},
  number = {6},
  pages = {67-72},
  doi = {10.11648/j.bmb.20170206.11},
  url = {https://doi.org/10.11648/j.bmb.20170206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20170206.11},
  abstract = {This study was undertaken to screen for Lactic Acid Bacteria (LAB) from indigenous fermented seeds; dawadawa. Isolation of LAB was carried out on MRS Agar. Biochemical, morphological and physiological examinations according to the Bergeys manual were used to identify the bacterial isolates. Six bacterial strains were obtained and given the designations DN, DO, DJ, DT, DG and DH respectively. The obtained colonies were all capable of fermenting lactose, sucrose, trehalose, glucose, maltose and lactulose as the sole carbon source in the broth. Morphological examination revealed that the isolates obtained were non-spore forming gram positive colonies ranging from creamy to white, clear, cocci and rod shaped, smooth textured and flat elevation with transparent opacity. Their growth profile study revealed that all isolates appeared to be mesophilc with progressive growth patterns observed between 24-96 hours at the same parameters for incubation. Isolates DO and DJ were observed to exhibit the fastest growth as determined by measuring optical density of the cells in broth using spectrophotometer at 550nm. Conclusions from the observed experiment indicate that isolated LABs from dawadawa could be applied in other probiotics as starter cultures and be improved for other commercial uses.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Screening of Indigenous Bacterial Isolates from Dawadawa (African Locust Beans) in North Central Nigeria
AU  - Oyetola Moradeke Toyosi
AU  - Agarry Olubunmi
AU  - Oyetola Simeon
Y1  - 2017/11/05
PY  - 2017
N1  - https://doi.org/10.11648/j.bmb.20170206.11
DO  - 10.11648/j.bmb.20170206.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 67
EP  - 72
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20170206.11
AB  - This study was undertaken to screen for Lactic Acid Bacteria (LAB) from indigenous fermented seeds; dawadawa. Isolation of LAB was carried out on MRS Agar. Biochemical, morphological and physiological examinations according to the Bergeys manual were used to identify the bacterial isolates. Six bacterial strains were obtained and given the designations DN, DO, DJ, DT, DG and DH respectively. The obtained colonies were all capable of fermenting lactose, sucrose, trehalose, glucose, maltose and lactulose as the sole carbon source in the broth. Morphological examination revealed that the isolates obtained were non-spore forming gram positive colonies ranging from creamy to white, clear, cocci and rod shaped, smooth textured and flat elevation with transparent opacity. Their growth profile study revealed that all isolates appeared to be mesophilc with progressive growth patterns observed between 24-96 hours at the same parameters for incubation. Isolates DO and DJ were observed to exhibit the fastest growth as determined by measuring optical density of the cells in broth using spectrophotometer at 550nm. Conclusions from the observed experiment indicate that isolated LABs from dawadawa could be applied in other probiotics as starter cultures and be improved for other commercial uses.
VL  - 2
IS  - 6
ER  -

Copy | Download