The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis

Larisa Mikhailovna Somova; Irina Nikolaevna Lyapun; Elena Igorevna Drobot; Felix Nikolayevich Shubin; Natalya Gennadyevna Plekhova

doi:doi:10.11648/j.bmb.20180303.11

The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis

Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova

Published in Biochemistry and Molecular Biology (Volume 3, Issue 3)

Received: 18 September 2018 Accepted: 5 October 2018 Published: 1 November 2018

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Abstract

In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82⁺: 48⁺, containing two plasmids pVM 82 and pYV; type 82⁺: 48- containing single pVM 82 plasmid; type 48⁺: 82^- containing single pYV plasmid; plasmid-free type 48^-: 82^-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.

Published in	Biochemistry and Molecular Biology (Volume 3, Issue 3)
DOI	10.11648/j.bmb.20180303.11
Page(s)	49-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Metabolic Activity, Innate Immunity Cells, ATPase, Lactate Dehydrogenase, Myelo Peroxidase, Nitric Oxide Metabolites, Stimulation Index (Т), Variability, Plasmid Types of Yersinia Pseudotu Berculosis

References

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Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. (2018). The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochemistry and Molecular Biology, 3(3), 49-55. https://doi.org/10.11648/j.bmb.20180303.11

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Larisa Mikhailovna Somova; Irina Nikolaevna Lyapun; Elena Igorevna Drobot; Felix Nikolayevich Shubin; Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem. Mol. Biol. 2018, 3(3), 49-55. doi: 10.11648/j.bmb.20180303.11

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Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem Mol Biol. 2018;3(3):49-55. doi: 10.11648/j.bmb.20180303.11

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@article{10.11648/j.bmb.20180303.11,
  author = {Larisa Mikhailovna Somova and Irina Nikolaevna Lyapun and Elena Igorevna Drobot and Felix Nikolayevich Shubin and Natalya Gennadyevna Plekhova},
  title = {The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {3},
  pages = {49-55},
  doi = {10.11648/j.bmb.20180303.11},
  url = {https://doi.org/10.11648/j.bmb.20180303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180303.11},
  abstract = {In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.},
 year = {2018}
}

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TY  - JOUR
T1  - The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis
AU  - Larisa Mikhailovna Somova
AU  - Irina Nikolaevna Lyapun
AU  - Elena Igorevna Drobot
AU  - Felix Nikolayevich Shubin
AU  - Natalya Gennadyevna Plekhova
Y1  - 2018/11/01
PY  - 2018
N1  - https://doi.org/10.11648/j.bmb.20180303.11
DO  - 10.11648/j.bmb.20180303.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 49
EP  - 55
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180303.11
AB  - In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.
VL  - 3
IS  - 3
ER  -

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Author Information

Larisa Mikhailovna Somova

Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Irina Nikolaevna Lyapun

Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Elena Igorevna Drobot

Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Felix Nikolayevich Shubin

Ministry of Science and Higher Education, Somov Institute of Epidemiology and Microbiology, Vladivostok, Russian Federation
Natalya Gennadyevna Plekhova

Ministry of Health of Russia, Pacific Medical University, Vladivostok, Russian Federation

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Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. (2018). The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochemistry and Molecular Biology, 3(3), 49-55. https://doi.org/10.11648/j.bmb.20180303.11

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ACS Style

Larisa Mikhailovna Somova; Irina Nikolaevna Lyapun; Elena Igorevna Drobot; Felix Nikolayevich Shubin; Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem. Mol. Biol. 2018, 3(3), 49-55. doi: 10.11648/j.bmb.20180303.11

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AMA Style

Larisa Mikhailovna Somova, Irina Nikolaevna Lyapun, Elena Igorevna Drobot, Felix Nikolayevich Shubin, Natalya Gennadyevna Plekhova. The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis. Biochem Mol Biol. 2018;3(3):49-55. doi: 10.11648/j.bmb.20180303.11

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@article{10.11648/j.bmb.20180303.11,
  author = {Larisa Mikhailovna Somova and Irina Nikolaevna Lyapun and Elena Igorevna Drobot and Felix Nikolayevich Shubin and Natalya Gennadyevna Plekhova},
  title = {The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis},
  journal = {Biochemistry and Molecular Biology},
  volume = {3},
  number = {3},
  pages = {49-55},
  doi = {10.11648/j.bmb.20180303.11},
  url = {https://doi.org/10.11648/j.bmb.20180303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20180303.11},
  abstract = {In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.},
 year = {2018}
}

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TY  - JOUR
T1  - The Metabolic Activity of Innate Immunity Cells in Experimental Infection Caused by Various Plasmid Types of Yersinia Pseudotuberculosis
AU  - Larisa Mikhailovna Somova
AU  - Irina Nikolaevna Lyapun
AU  - Elena Igorevna Drobot
AU  - Felix Nikolayevich Shubin
AU  - Natalya Gennadyevna Plekhova
Y1  - 2018/11/01
PY  - 2018
N1  - https://doi.org/10.11648/j.bmb.20180303.11
DO  - 10.11648/j.bmb.20180303.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 49
EP  - 55
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20180303.11
AB  - In a comparative aspect, the functional state of inflammation effector cells in animals infected with various plasmid types of Yersinia pseudotuberculosis was studied. The metabolic activity of peritoneal exudate cells has been investigated in an experimental infection caused by four plasmid types of Y. pseudotuberculosis: type 82+: 48+, containing two plasmids pVM 82 and pYV; type 82+: 48- containing single pVM 82 plasmid; type 48+: 82- containing single pYV plasmid; plasmid-free type 48-: 82-. The parameters of enzyme activity (ATP-ase, 5'-nucleotidase, lactate dehydrogenase, myeloperoxidase) and the level of nitric oxide metabolites were determined. The variability of the metabolic activity of the cells in the inflammatory focus (peritoneal exudate containing neutrophils and macrophages) in infected animals has been established. In response to infection with Y. pseudotuberculosis strain containing two plasmids pYV and pVM82, the production of the nitric oxide metabolites, rather than the active forms of oxygen, had the primary importance in providing the bactericidal potential of phagocytes, compared to animals infected with a strain containing a single pVM82 plasmid. It was concluded that a special biological effect associated with the pVM 82 plasmid available in the Far Eastern strains of the causative agent of epidemic pseudotuberculosis (Far Eastern scarlet-like fever) was involved in the provision of predominantly nitroxide-dependent bactericidal mechanisms of innate immunity cells in this infection.
VL  - 3
IS  - 3
ER  -

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