First Report of Peanut Stripe Virus Infecting Groundnut (<i>Arachis hypogaea</i> L.) in Western Kenya

Anthony Simiyu Mabele; Fredrick Wotia

doi:doi:10.11648/j.bmb.20210601.13

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First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya

Anthony Simiyu Mabele, Fredrick Wotia

Published in Biochemistry and Molecular Biology (Volume 6, Issue 1)

Received: 30 January 2021 Accepted: 14 February 2021 Published: 26 February 2021

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Abstract

Peanut (Arachis hypogaea) is a leguminous food crop in Kenya, whose yields are probably affected by Peanut stripe virus (PStV) belonging to the genus Potyvirus and family Potyviridae, that causes Peanut stripe disease (PSD), also referred to as groundnut stripe disease (GSD). PSD/GSD is rapidly spread through seed and mechanically transmitted in a non-persistent manner by aphid vectors causing 30-100% decrease in production. Different isolates of PStV induce different symptoms in groundnut ranging from striping, ring spots, vein banding and oakleaf mosaic. PStV occurrence in Kenya has neither been reported nor its resistance identified in commercial cultivars of groundnut in research stations. This informs the need for resistance breeding programmes of wild genotypes to be explored as the only practical solution. This study reports the first occurrence, distribution and molecular detection of PStV infecting groundnut in western Kenya, which is crucial in developing appropriate crop protection and plant health management strategies for this new disease. A survey of PSD was conducted in western Kenya among the four counties of Bungoma, Busia, Kisumu and Kisii during the short rains season of 2019. A total of 14 symptomatic leaf samples were selected from the collected samples and preserved until use. Total RNA was extracted from the symptomatic leaf samples using GeneJET Plant RNA Purification Mini Kit according to the manufacturers’ protocol. Two-step RT-PCR detection of PStV was done using specific primers that yielded the expected 454 bp. DNA libraries were prepared and sequenced using the Sanger sequencing platform, but produced poor quality sequences after performing sequence quality check using BioEdit workbench that based on the low quality peaks of the electropherograms generated, therefore recommend for further research. To our knowledge, this is the ﬁrst report of the occurrence, distribution and molecular diagnostics of PStV in Kenya. Further studies are needed to assess the molecular and biological diversity of PStV strains, the spatial distribution and the incidence of this emerging groundnut disease in Kenya.

Published in	Biochemistry and Molecular Biology (Volume 6, Issue 1)
DOI	10.11648/j.bmb.20210601.13
Page(s)	11-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Occurrence, Peanut stripe virus, Potyvirus, Two-step RT-PCR, Western Kenya

References

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APA Style

Anthony Simiyu Mabele, Fredrick Wotia. (2021). First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochemistry and Molecular Biology, 6(1), 11-18. https://doi.org/10.11648/j.bmb.20210601.13

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ACS Style

Anthony Simiyu Mabele; Fredrick Wotia. First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochem. Mol. Biol. 2021, 6(1), 11-18. doi: 10.11648/j.bmb.20210601.13

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AMA Style

Anthony Simiyu Mabele, Fredrick Wotia. First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochem Mol Biol. 2021;6(1):11-18. doi: 10.11648/j.bmb.20210601.13

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@article{10.11648/j.bmb.20210601.13,
  author = {Anthony Simiyu Mabele and Fredrick Wotia},
  title = {First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {1},
  pages = {11-18},
  doi = {10.11648/j.bmb.20210601.13},
  url = {https://doi.org/10.11648/j.bmb.20210601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210601.13},
  abstract = {Peanut (Arachis hypogaea) is a leguminous food crop in Kenya, whose yields are probably affected by Peanut stripe virus (PStV) belonging to the genus Potyvirus and family Potyviridae, that causes Peanut stripe disease (PSD), also referred to as groundnut stripe disease (GSD). PSD/GSD is rapidly spread through seed and mechanically transmitted in a non-persistent manner by aphid vectors causing 30-100% decrease in production. Different isolates of PStV induce different symptoms in groundnut ranging from striping, ring spots, vein banding and oakleaf mosaic. PStV occurrence in Kenya has neither been reported nor its resistance identified in commercial cultivars of groundnut in research stations. This informs the need for resistance breeding programmes of wild genotypes to be explored as the only practical solution. This study reports the first occurrence, distribution and molecular detection of PStV infecting groundnut in western Kenya, which is crucial in developing appropriate crop protection and plant health management strategies for this new disease. A survey of PSD was conducted in western Kenya among the four counties of Bungoma, Busia, Kisumu and Kisii during the short rains season of 2019. A total of 14 symptomatic leaf samples were selected from the collected samples and preserved until use. Total RNA was extracted from the symptomatic leaf samples using GeneJET Plant RNA Purification Mini Kit according to the manufacturers’ protocol. Two-step RT-PCR detection of PStV was done using specific primers that yielded the expected 454 bp. DNA libraries were prepared and sequenced using the Sanger sequencing platform, but produced poor quality sequences after performing sequence quality check using BioEdit workbench that based on the low quality peaks of the electropherograms generated, therefore recommend for further research. To our knowledge, this is the ﬁrst report of the occurrence, distribution and molecular diagnostics of PStV in Kenya. Further studies are needed to assess the molecular and biological diversity of PStV strains, the spatial distribution and the incidence of this emerging groundnut disease in Kenya.},
 year = {2021}
}

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TY  - JOUR
T1  - First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya
AU  - Anthony Simiyu Mabele
AU  - Fredrick Wotia
Y1  - 2021/02/26
PY  - 2021
N1  - https://doi.org/10.11648/j.bmb.20210601.13
DO  - 10.11648/j.bmb.20210601.13
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 11
EP  - 18
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20210601.13
AB  - Peanut (Arachis hypogaea) is a leguminous food crop in Kenya, whose yields are probably affected by Peanut stripe virus (PStV) belonging to the genus Potyvirus and family Potyviridae, that causes Peanut stripe disease (PSD), also referred to as groundnut stripe disease (GSD). PSD/GSD is rapidly spread through seed and mechanically transmitted in a non-persistent manner by aphid vectors causing 30-100% decrease in production. Different isolates of PStV induce different symptoms in groundnut ranging from striping, ring spots, vein banding and oakleaf mosaic. PStV occurrence in Kenya has neither been reported nor its resistance identified in commercial cultivars of groundnut in research stations. This informs the need for resistance breeding programmes of wild genotypes to be explored as the only practical solution. This study reports the first occurrence, distribution and molecular detection of PStV infecting groundnut in western Kenya, which is crucial in developing appropriate crop protection and plant health management strategies for this new disease. A survey of PSD was conducted in western Kenya among the four counties of Bungoma, Busia, Kisumu and Kisii during the short rains season of 2019. A total of 14 symptomatic leaf samples were selected from the collected samples and preserved until use. Total RNA was extracted from the symptomatic leaf samples using GeneJET Plant RNA Purification Mini Kit according to the manufacturers’ protocol. Two-step RT-PCR detection of PStV was done using specific primers that yielded the expected 454 bp. DNA libraries were prepared and sequenced using the Sanger sequencing platform, but produced poor quality sequences after performing sequence quality check using BioEdit workbench that based on the low quality peaks of the electropherograms generated, therefore recommend for further research. To our knowledge, this is the ﬁrst report of the occurrence, distribution and molecular diagnostics of PStV in Kenya. Further studies are needed to assess the molecular and biological diversity of PStV strains, the spatial distribution and the incidence of this emerging groundnut disease in Kenya.
VL  - 6
IS  - 1
ER  -

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Author Information

Anthony Simiyu Mabele

Department of Agriculture and Land Use Management (ALUM), Masinde Muliro University of Science and Technology (MMUST), Kakamega, Kenya
Fredrick Wotia

Department of Agriculture and Land Use Management (ALUM), Masinde Muliro University of Science and Technology (MMUST), Kakamega, Kenya

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APA Style

Anthony Simiyu Mabele, Fredrick Wotia. (2021). First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochemistry and Molecular Biology, 6(1), 11-18. https://doi.org/10.11648/j.bmb.20210601.13

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ACS Style

Anthony Simiyu Mabele; Fredrick Wotia. First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochem. Mol. Biol. 2021, 6(1), 11-18. doi: 10.11648/j.bmb.20210601.13

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AMA Style

Anthony Simiyu Mabele, Fredrick Wotia. First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya. Biochem Mol Biol. 2021;6(1):11-18. doi: 10.11648/j.bmb.20210601.13

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@article{10.11648/j.bmb.20210601.13,
  author = {Anthony Simiyu Mabele and Fredrick Wotia},
  title = {First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {1},
  pages = {11-18},
  doi = {10.11648/j.bmb.20210601.13},
  url = {https://doi.org/10.11648/j.bmb.20210601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210601.13},
  abstract = {Peanut (Arachis hypogaea) is a leguminous food crop in Kenya, whose yields are probably affected by Peanut stripe virus (PStV) belonging to the genus Potyvirus and family Potyviridae, that causes Peanut stripe disease (PSD), also referred to as groundnut stripe disease (GSD). PSD/GSD is rapidly spread through seed and mechanically transmitted in a non-persistent manner by aphid vectors causing 30-100% decrease in production. Different isolates of PStV induce different symptoms in groundnut ranging from striping, ring spots, vein banding and oakleaf mosaic. PStV occurrence in Kenya has neither been reported nor its resistance identified in commercial cultivars of groundnut in research stations. This informs the need for resistance breeding programmes of wild genotypes to be explored as the only practical solution. This study reports the first occurrence, distribution and molecular detection of PStV infecting groundnut in western Kenya, which is crucial in developing appropriate crop protection and plant health management strategies for this new disease. A survey of PSD was conducted in western Kenya among the four counties of Bungoma, Busia, Kisumu and Kisii during the short rains season of 2019. A total of 14 symptomatic leaf samples were selected from the collected samples and preserved until use. Total RNA was extracted from the symptomatic leaf samples using GeneJET Plant RNA Purification Mini Kit according to the manufacturers’ protocol. Two-step RT-PCR detection of PStV was done using specific primers that yielded the expected 454 bp. DNA libraries were prepared and sequenced using the Sanger sequencing platform, but produced poor quality sequences after performing sequence quality check using BioEdit workbench that based on the low quality peaks of the electropherograms generated, therefore recommend for further research. To our knowledge, this is the ﬁrst report of the occurrence, distribution and molecular diagnostics of PStV in Kenya. Further studies are needed to assess the molecular and biological diversity of PStV strains, the spatial distribution and the incidence of this emerging groundnut disease in Kenya.},
 year = {2021}
}

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TY  - JOUR
T1  - First Report of Peanut Stripe Virus Infecting Groundnut (Arachis hypogaea L.) in Western Kenya
AU  - Anthony Simiyu Mabele
AU  - Fredrick Wotia
Y1  - 2021/02/26
PY  - 2021
N1  - https://doi.org/10.11648/j.bmb.20210601.13
DO  - 10.11648/j.bmb.20210601.13
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 11
EP  - 18
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20210601.13
AB  - Peanut (Arachis hypogaea) is a leguminous food crop in Kenya, whose yields are probably affected by Peanut stripe virus (PStV) belonging to the genus Potyvirus and family Potyviridae, that causes Peanut stripe disease (PSD), also referred to as groundnut stripe disease (GSD). PSD/GSD is rapidly spread through seed and mechanically transmitted in a non-persistent manner by aphid vectors causing 30-100% decrease in production. Different isolates of PStV induce different symptoms in groundnut ranging from striping, ring spots, vein banding and oakleaf mosaic. PStV occurrence in Kenya has neither been reported nor its resistance identified in commercial cultivars of groundnut in research stations. This informs the need for resistance breeding programmes of wild genotypes to be explored as the only practical solution. This study reports the first occurrence, distribution and molecular detection of PStV infecting groundnut in western Kenya, which is crucial in developing appropriate crop protection and plant health management strategies for this new disease. A survey of PSD was conducted in western Kenya among the four counties of Bungoma, Busia, Kisumu and Kisii during the short rains season of 2019. A total of 14 symptomatic leaf samples were selected from the collected samples and preserved until use. Total RNA was extracted from the symptomatic leaf samples using GeneJET Plant RNA Purification Mini Kit according to the manufacturers’ protocol. Two-step RT-PCR detection of PStV was done using specific primers that yielded the expected 454 bp. DNA libraries were prepared and sequenced using the Sanger sequencing platform, but produced poor quality sequences after performing sequence quality check using BioEdit workbench that based on the low quality peaks of the electropherograms generated, therefore recommend for further research. To our knowledge, this is the ﬁrst report of the occurrence, distribution and molecular diagnostics of PStV in Kenya. Further studies are needed to assess the molecular and biological diversity of PStV strains, the spatial distribution and the incidence of this emerging groundnut disease in Kenya.
VL  - 6
IS  - 1
ER  -

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