PCR-RFLP Fails to Reveal Variability Within <i>Schistosoma haematobium</i> Detected in Loum (Cameroon) by Isoelectrofocusing Technique

Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente; Wilfred Fon Mbacham

doi:doi:10.11648/j.bmb.20210603.11

PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, Wilfred Fon Mbacham

Published in Biochemistry and Molecular Biology (Volume 6, Issue 3)

Received: 4 June 2021 Accepted: 25 June 2021 Published: 9 July 2021

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Abstract

Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.

Published in	Biochemistry and Molecular Biology (Volume 6, Issue 3)
DOI	10.11648/j.bmb.20210603.11
Page(s)	35-39
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Schistosoma haematobium, Genetic Variability, PCR-RFLP, Loum, Cameroon

References

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Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. (2021). PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochemistry and Molecular Biology, 6(3), 35-39. https://doi.org/10.11648/j.bmb.20210603.11

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Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem. Mol. Biol. 2021, 6(3), 35-39. doi: 10.11648/j.bmb.20210603.11

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Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem Mol Biol. 2021;6(3):35-39. doi: 10.11648/j.bmb.20210603.11

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@article{10.11648/j.bmb.20210603.11,
  author = {Luogbou Nzu Deguy D’or and Palmer Masumbe Netongo and Nguemaïm Ngoufo Flore and Marc Kenmogne Kouam and Louis-Albert Tchuem-Tchuente and Wilfred Fon Mbacham},
  title = {PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {3},
  pages = {35-39},
  doi = {10.11648/j.bmb.20210603.11},
  url = {https://doi.org/10.11648/j.bmb.20210603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210603.11},
  abstract = {Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.},
 year = {2021}
}

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TY - JOUR
T1 - PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique
AU - Luogbou Nzu Deguy D’or
AU - Palmer Masumbe Netongo
AU - Nguemaïm Ngoufo Flore
AU - Marc Kenmogne Kouam
AU - Louis-Albert Tchuem-Tchuente
AU - Wilfred Fon Mbacham
Y1 - 2021/07/09
PY - 2021
N1 - https://doi.org/10.11648/j.bmb.20210603.11
DO - 10.11648/j.bmb.20210603.11
T2 - Biochemistry and Molecular Biology
JF - Biochemistry and Molecular Biology
JO - Biochemistry and Molecular Biology
SP - 35
EP - 39
PB - Science Publishing Group
SN - 2575-5048
UR - https://doi.org/10.11648/j.bmb.20210603.11
AB - Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.
VL - 6
IS - 3
ER -

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Author Information

Luogbou Nzu Deguy D’or

Centre for Schistosomiasis & Parasitology, Yaounde, Cameroon
Palmer Masumbe Netongo

The Laboratory for Public Health Research Biotechnologies, University of Yaounde I, Yaounde, Cameroon
Nguemaïm Ngoufo Flore
Marc Kenmogne Kouam

Department of Animal Sciences, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Cameroon
Louis-Albert Tchuem-Tchuente

Centre for Schistosomiasis & Parasitology, Yaounde, Cameroon
Wilfred Fon Mbacham

The Laboratory for Public Health Research Biotechnologies, University of Yaounde I, Yaounde, Cameroon

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APA Style

Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. (2021). PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochemistry and Molecular Biology, 6(3), 35-39. https://doi.org/10.11648/j.bmb.20210603.11

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Luogbou Nzu Deguy D’or; Palmer Masumbe Netongo; Nguemaïm Ngoufo Flore; Marc Kenmogne Kouam; Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem. Mol. Biol. 2021, 6(3), 35-39. doi: 10.11648/j.bmb.20210603.11

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Luogbou Nzu Deguy D’or, Palmer Masumbe Netongo, Nguemaïm Ngoufo Flore, Marc Kenmogne Kouam, Louis-Albert Tchuem-Tchuente, et al. PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique. Biochem Mol Biol. 2021;6(3):35-39. doi: 10.11648/j.bmb.20210603.11

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@article{10.11648/j.bmb.20210603.11,
  author = {Luogbou Nzu Deguy D’or and Palmer Masumbe Netongo and Nguemaïm Ngoufo Flore and Marc Kenmogne Kouam and Louis-Albert Tchuem-Tchuente and Wilfred Fon Mbacham},
  title = {PCR-RFLP Fails to Reveal Variability Within Schistosoma haematobium Detected in Loum (Cameroon) by Isoelectrofocusing Technique},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {3},
  pages = {35-39},
  doi = {10.11648/j.bmb.20210603.11},
  url = {https://doi.org/10.11648/j.bmb.20210603.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210603.11},
  abstract = {Schistosoma haematobium, which causes urinary schistosomiasis in humans is responsible for the largest number of infections in the word. Genetic variability among parasite populations is an important factor in their potential for producing harmful effects on the human populations they infect. In many areas, S. haematobium is sympatric with related schistosome parasites (most of other mammals) (i.e., S. bovis, S. mattheei, S. curassoni, S. intercalatum, S. guineensis and S. margrebowiei). PCR-RFLP analysis of ITS-2 rDNA loci is an usefull tools to detect hybrids amongs Schistosoma haematobium group. Many studies have been carry out in the town of Loum (Cameroon) in order to characterize Schistosoma haematobium species from this locality. However, no study based on PCR-RFLP analysis succeeds to detect any genetic variability as reported before using electrofocusing (IEF) technique. PCR-RFLP analysis realised on 10 isolates of Schistosoma haematobium from Loum reveals a DNA fragment of 501 bp after amplification of ITS2 ribosomal gene. For all the samples, the enzymatic digestion of the mentioned DNA fragment gene with Taq I reveals two DNA bands of 158 bp and 199 bp which is characteristic of Schistosoma haematobium. In summary, molecular characterization of S. haematobium in Loum using PCR-RFLP approach reveals once more the absence of hybrids and no genetic variability. Further studies on a larger geographic scale involving many schools in Loum should be encouraged to screen more parasite isolates with different primers and molecular toolS. Information from such studies would provide better insight into the local lineages of S. haematobium. This knowledge might play a major role in establishing control strategies for urogenital schistosomiasis in Loum.},
 year = {2021}
}

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