@article{10.11648/j.bmb.20210603.15,
  author = {Vicky Jocelyne Ama Moor and Claude Venessa Kapya Ouandji and Madeleine Chantal Ngoungoure and Pascal Emmanuel Owona and Lohik Mbolang Nguegang and Magellan Guewo-Fokeng and Amazia Falmata and Aggons Batakeh and Constant Anatole Pieme},
  title = {Impact of Aqueous Extract of the Stem Bark of Anthocleista schweinfurthii Gilg (Loganiaceae) on Some Parameters of the Reproductive Function of Adult Albino Male Rats},
  journal = {Biochemistry and Molecular Biology},
  volume = {6},
  number = {3},
  pages = {67-78},
  doi = {10.11648/j.bmb.20210603.15},
  url = {https://doi.org/10.11648/j.bmb.20210603.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20210603.15},
  abstract = {Both modern and traditional medicine options are being practiced worldwide to alleviate and treat diseases. However, some drugs used for certain diseases have deleterious effects on other systems and functions of the body. This study aims to investigate the impact of aqueous extracts of the stem bark of Anthocleista schweinfurthii on some parameters of the reproductive function of adult albino male rats, and to perform the phytochemical screening of the extract. To achieve this objective, 18 male rats aged 60 days and weighing 150 g were divided into three groups of six rats each and were orally treated for 28 days with: Distilled water (10 mL/kg) for group 1 (control group), aqueous extract of A. schweinfurthii at respective doses of 300 and 400 mg/kg for groups 2 and 3 (the two test groups). At the end of the 28-day period, the animals were anesthetized and decapitated. Blood samples were collected for serum analysis. Some androgen-dependent organs were collected and weighed. The epididymis and sperm were collected for evaluation of sperm count, mobility and viability. A sample of each organ (testis, epididymis and seminal vesicle) was homogenised for biochemical analysis and other parts were kept in Bouin’s solution for histological examinations. The phytochemical screening revealed the presence of many secondary plant metabolites in the extract, including alkaloids, flavonoids and saponins. The results obtained show that the aqueous extract induced a significant reduction of the relative weight of the testis, the epididymis and the seminal vesicles. A reduction in sperm density, mobility and viability were also observed. The rates of testicular, seminal vesicle and epididymal proteins, serum cholesterol and seminal vesicle fructose decreased significantly. Otherwise, plant extracts increased testicular cholesterol concentration. Anthocleista schweinfurthii caused a significant decrease in serum gonadotrophin levels (LH, FSH) and serum testosterone concentrations and an improvement in parameters of oxidative stress. In addition, an alteration in the structure of the androgen-dependent organs was depicted on histological sections. From these findings, it is concluded that Anthocleista schweinfurthii stem bark alters some parameters of the reproductive function of adult albino male rats and suggest a limitation of their use in male subjects.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Impact of Aqueous Extract of the Stem Bark of Anthocleista schweinfurthii Gilg (Loganiaceae) on Some Parameters of the Reproductive Function of Adult Albino Male Rats
AU  - Vicky Jocelyne Ama Moor
AU  - Claude Venessa Kapya Ouandji
AU  - Madeleine Chantal Ngoungoure
AU  - Pascal Emmanuel Owona
AU  - Lohik Mbolang Nguegang
AU  - Magellan Guewo-Fokeng
AU  - Amazia Falmata
AU  - Aggons Batakeh
AU  - Constant Anatole Pieme
Y1  - 2021/08/13
PY  - 2021
N1  - https://doi.org/10.11648/j.bmb.20210603.15
DO  - 10.11648/j.bmb.20210603.15
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 67
EP  - 78
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20210603.15
AB  - Both modern and traditional medicine options are being practiced worldwide to alleviate and treat diseases. However, some drugs used for certain diseases have deleterious effects on other systems and functions of the body. This study aims to investigate the impact of aqueous extracts of the stem bark of Anthocleista schweinfurthii on some parameters of the reproductive function of adult albino male rats, and to perform the phytochemical screening of the extract. To achieve this objective, 18 male rats aged 60 days and weighing 150 g were divided into three groups of six rats each and were orally treated for 28 days with: Distilled water (10 mL/kg) for group 1 (control group), aqueous extract of A. schweinfurthii at respective doses of 300 and 400 mg/kg for groups 2 and 3 (the two test groups). At the end of the 28-day period, the animals were anesthetized and decapitated. Blood samples were collected for serum analysis. Some androgen-dependent organs were collected and weighed. The epididymis and sperm were collected for evaluation of sperm count, mobility and viability. A sample of each organ (testis, epididymis and seminal vesicle) was homogenised for biochemical analysis and other parts were kept in Bouin’s solution for histological examinations. The phytochemical screening revealed the presence of many secondary plant metabolites in the extract, including alkaloids, flavonoids and saponins. The results obtained show that the aqueous extract induced a significant reduction of the relative weight of the testis, the epididymis and the seminal vesicles. A reduction in sperm density, mobility and viability were also observed. The rates of testicular, seminal vesicle and epididymal proteins, serum cholesterol and seminal vesicle fructose decreased significantly. Otherwise, plant extracts increased testicular cholesterol concentration. Anthocleista schweinfurthii caused a significant decrease in serum gonadotrophin levels (LH, FSH) and serum testosterone concentrations and an improvement in parameters of oxidative stress. In addition, an alteration in the structure of the androgen-dependent organs was depicted on histological sections. From these findings, it is concluded that Anthocleista schweinfurthii stem bark alters some parameters of the reproductive function of adult albino male rats and suggest a limitation of their use in male subjects.
VL  - 6
IS  - 3
ER  - 

Copy | Download