Comparison of Spray and Point Inoculation with <i>Fusarium graminearum</i> to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress

Victor Chijioke Okereke

doi:doi:10.11648/j.cbb.20170501.11

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Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress

Victor Chijioke Okereke

Published in Computational Biology and Bioinformatics (Volume 5, Issue 1)

Received: 10 August 2016 Accepted: 24 August 2016 Published: 13 April 2017

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Abstract

Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.

Published in	Computational Biology and Bioinformatics (Volume 5, Issue 1)
DOI	10.11648/j.cbb.20170501.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Point, Spray, Inoculation, Mercia 1, Mercia 2, Fusarium graminearum, Type 1 Resistance, Type 2 Resistance

References

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[2]	Gosman, N., Bayles, R., Jennings, P., Kirby, J., Nicholson, P. 2007. Evaluation and characterization of resistance to Fusarium head blight caused by Fusarium culmorum in UK winter wheat cultivars. Plant Pathology 56: 264-276.
[3]	Mesterhazy, A. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breeding 114: 377-386.
[4]	Stack, R. W. 2000. Return of an old problem: Fusarium head blight of small grains. Plant Health Review, June 2000.
[5]	Schroeder, H. W., Christensen, J. J. 1963. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53: 831-838.
[6]	Bai, G. H., Plattner, R., Desjardins, A., Kolb, F. S., Jones, S. 2001. Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding 120: 1-6.
[7]	Miedaner, T., Moldovan, M., Ittu, M. 2003. Comparison of spray and point inoculation to assess resistance to Fusarium head blight in a multi environment wheat trial. Phytopathology 93: 1068-1072.
[8]	Malbrán, I., Mourelos, C. A., Girotti, J. R., Aulicino, M. B., Balatti, P. A., Lori, G. A. 2012. Aggressiveness variation of Fusarium graminearum isolates from Argentina following point inoculation of field grown wheat spikes. Crop Protection 42: 234-243.
[9]	Miedaner, T. 1997. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding 116: 201-220.
[10]	Rudd, J. C., Horsley, R. D., McKendry, A. L. and Elias, E. M. J. C. 2001. Host Plant Resistance Genes for Fusarium Head Blight: Sources, Mechanisms and Utility in Conventional Breeding Systems. Crop Science 41: 620–627.
[11]	Desmeules, J., Paulitz, T., Rioux, S., O’Donoughue, L., Mather, D. 2001. Fusarium head blight symptom development in spring what genotypes after inoculation with point and spray methods. Proceeding of Canadian Workshop on Fusarium head blight. Ottawa, November 3-5, Pp 50.
[12]	Bai, G., Shaner, G. 1994. Scab of wheat: Prospect for control. Plant Disease 78: 760-766.13.
[13]	Zadoks, J. C., Chang, T. T, Konza, C. F. 1974. A decimal code for the growth stages of cereals. Weed Research 14: 415-421.
[14]	Bai, G., Shaner, G. 2004. Management and resistance in wheat and barley to Fusarium head blight. Annual Review of Phytopathology 42: 135-161.
[15]	Thompson, C. J., 2010. Evaluation of an inoculation method and quantitative trait loci for Fusarium head blight resistance in wheat. Unpublished Master of Science Thesis, University of Illinois at Urbana-Champaign.
[16]	Mesterházy, A., Bartok, T., Kaszonyi G., Varga, M., Toth, B., Varga., J. 2005. Common resistance to different Fusarium spp. Causing Fusarium head blight in wheat. European Journal of Plant Pathology 112: 267-281.

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APA Style

Victor Chijioke Okereke. (2017). Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Computational Biology and Bioinformatics, 5(1), 1-5. https://doi.org/10.11648/j.cbb.20170501.11

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ACS Style

Victor Chijioke Okereke. Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Comput. Biol. Bioinform. 2017, 5(1), 1-5. doi: 10.11648/j.cbb.20170501.11

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AMA Style

Victor Chijioke Okereke. Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Comput Biol Bioinform. 2017;5(1):1-5. doi: 10.11648/j.cbb.20170501.11

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@article{10.11648/j.cbb.20170501.11,
  author = {Victor Chijioke Okereke},
  title = {Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress},
  journal = {Computational Biology and Bioinformatics},
  volume = {5},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.cbb.20170501.11},
  url = {https://doi.org/10.11648/j.cbb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20170501.11},
  abstract = {Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.},
 year = {2017}
}

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TY  - JOUR
T1  - Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress
AU  - Victor Chijioke Okereke
Y1  - 2017/04/13
PY  - 2017
N1  - https://doi.org/10.11648/j.cbb.20170501.11
DO  - 10.11648/j.cbb.20170501.11
T2  - Computational Biology and Bioinformatics
JF  - Computational Biology and Bioinformatics
JO  - Computational Biology and Bioinformatics
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-8281
UR  - https://doi.org/10.11648/j.cbb.20170501.11
AB  - Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.
VL  - 5
IS  - 1
ER  -

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Author Information

Victor Chijioke Okereke

Department of Crop and Soil Science, University of Port Harcourt, Port Harcourt, Nigeria

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APA Style

Victor Chijioke Okereke. (2017). Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Computational Biology and Bioinformatics, 5(1), 1-5. https://doi.org/10.11648/j.cbb.20170501.11

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ACS Style

Victor Chijioke Okereke. Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Comput. Biol. Bioinform. 2017, 5(1), 1-5. doi: 10.11648/j.cbb.20170501.11

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AMA Style

Victor Chijioke Okereke. Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress. Comput Biol Bioinform. 2017;5(1):1-5. doi: 10.11648/j.cbb.20170501.11

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@article{10.11648/j.cbb.20170501.11,
  author = {Victor Chijioke Okereke},
  title = {Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress},
  journal = {Computational Biology and Bioinformatics},
  volume = {5},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.cbb.20170501.11},
  url = {https://doi.org/10.11648/j.cbb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20170501.11},
  abstract = {Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Comparison of Spray and Point Inoculation with Fusarium graminearum to Evaluate FHB Disease in Two Winter Wheat Genotypes Under Temperature Stress
AU  - Victor Chijioke Okereke
Y1  - 2017/04/13
PY  - 2017
N1  - https://doi.org/10.11648/j.cbb.20170501.11
DO  - 10.11648/j.cbb.20170501.11
T2  - Computational Biology and Bioinformatics
JF  - Computational Biology and Bioinformatics
JO  - Computational Biology and Bioinformatics
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-8281
UR  - https://doi.org/10.11648/j.cbb.20170501.11
AB  - Two near isogenic lines of wheat; Mercia 1 (Rht-B1b) and Mercia 2 (Rht-D1b) were evaluated for Type I and Type II resistance using point or spray inoculation and incubated under controlled environment. The experiment was a complete factorial combination of 2 x 4 x 2 x 4 {2 genotypes, 4 inoculation treatments (spray + Fusarium, spray + SDW, point + Fusarium, point + SDW) x 2 temperatures} and 4 randomised replicates. Wheat spikes were sprayed with a single spore isolate of F. graminearum 4 days after the start of flowering and transferred to controlled environment cabinets set at either 23/15°C or 28/20°C for 14 days and then taken outside to mature. Results reveals that genotype showed no difference in FHB severity in both spray inoculation and point inoculation but the temperature main effect only approached significance (P=0.071) with low temperature increasing FHB severity following point inoculation. High temperature significantly (P<0.001) increased DON concentration in spray inoculation, contrasted the effect in point inoculation. However, the amount of DON per grain showed no significant (P>0.05) effect. Grains per spike was significantly (P<0.011) reduced by 25% following spray when compared with point inoculation, but showed no significant (P>0.05) effect of temperature. On average, Fusarium infection significantly (P<0.001) reduced wheat grain weight by 28% when compared with uninoculated control.
VL  - 5
IS  - 1
ER  -

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