Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope

Vladimir Boris Karpov

doi:doi:10.11648/j.cbb.20170505.11

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Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope

Vladimir Boris Karpov

Published in Computational Biology and Bioinformatics (Volume 5, Issue 5)

Received: 29 October 2016 Accepted: 13 October 2017 Published: 20 November 2017

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Abstract

The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 µm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.

Published in	Computational Biology and Bioinformatics (Volume 5, Issue 5)
DOI	10.11648/j.cbb.20170505.11
Page(s)	57-61
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Speckle-Noise, Fourier Holography, Mach – Zehnder Scheme, CCD Detector, Digital Image Reconstruction

References

[1]	M. Born, and E. Wojf, Principles of Optics, Oxford: Pergamon, 1964.
[2]	Light Microscopy in Biology. A Practical Approach, Ed. By Alan J. Lacey, Oxford: IRL, 1989.
[3]	Biological Science, Ed. By R. Soper B. Sc., F. I. Biol Cambridge: CAMBRIGE, 1984.
[4]	B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J. D. Watson, Molecular Biology of the Cell, New York: Garland, 1989.
[5]	J. W. Goodman, Statistical Optics, New York: Wiley, 1985.
[6]	D. Gabor, “Laser speckle and its elimination,” I. B. M. Journ. Res. Dev., vol. 14, 1970, pp. 509.
[7]	M. Francon, La Granularite Laser (Spekle) et ses Applications en Optique, Paris: Masson, 1978.
[8]	B. Ya. Zel’dovich, N. F. Pilipetsky, and V. V. Shkunov, Principles of Phase Conjugation, Berlin: Springer, 1985.
[9]	J. B. De Vellis, and G. O. Reynolds, Theory and Application of Holography, Mass: Addison - Wesley, Reading, 1967.
[10]	D. Gabor, “A new microscopy principle,” Nature, vol. 161, 1948, pp. 777 - 778.
[11]	W. S. Haddad, D. Cullen, J. C. Solem, J. W. Longworth, A. McPherson, K. Boyer, and Ch. K. Rhodes, “Fourier - transform holographic microscope,” Applied Optics, vol. 31, Issue 24, 1992, pp. 4973 - 4978.
[12]	J. T. Wintrop, and C. R. Worthington, “X - ray microscopy by successive Fourier transmission,” Physics Letters, vol. 15, 1965, pp. 124.
[13]	E. N. Leith, J. U patnieks, and K. Haines, “Microscopy by wavefront reconsruction,” Journal Optical Society of America, vol. 55, 1965, pp. 981.

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Vladimir Boris Karpov. (2017). Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Computational Biology and Bioinformatics, 5(5), 57-61. https://doi.org/10.11648/j.cbb.20170505.11

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ACS Style

Vladimir Boris Karpov. Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Comput. Biol. Bioinform. 2017, 5(5), 57-61. doi: 10.11648/j.cbb.20170505.11

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AMA Style

Vladimir Boris Karpov. Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Comput Biol Bioinform. 2017;5(5):57-61. doi: 10.11648/j.cbb.20170505.11

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@article{10.11648/j.cbb.20170505.11,
  author = {Vladimir Boris Karpov},
  title = {Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope},
  journal = {Computational Biology and Bioinformatics},
  volume = {5},
  number = {5},
  pages = {57-61},
  doi = {10.11648/j.cbb.20170505.11},
  url = {https://doi.org/10.11648/j.cbb.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20170505.11},
  abstract = {The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 µm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.},
 year = {2017}
}

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TY  - JOUR
T1  - Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope
AU  - Vladimir Boris Karpov
Y1  - 2017/11/20
PY  - 2017
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AB  - The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 µm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.
VL  - 5
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Author Information

Vladimir Boris Karpov

Coherent and Nonlinear Optics Department, A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, Russia

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APA Style

Vladimir Boris Karpov. (2017). Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Computational Biology and Bioinformatics, 5(5), 57-61. https://doi.org/10.11648/j.cbb.20170505.11

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ACS Style

Vladimir Boris Karpov. Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Comput. Biol. Bioinform. 2017, 5(5), 57-61. doi: 10.11648/j.cbb.20170505.11

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AMA Style

Vladimir Boris Karpov. Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope. Comput Biol Bioinform. 2017;5(5):57-61. doi: 10.11648/j.cbb.20170505.11

Copy | Download

@article{10.11648/j.cbb.20170505.11,
  author = {Vladimir Boris Karpov},
  title = {Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope},
  journal = {Computational Biology and Bioinformatics},
  volume = {5},
  number = {5},
  pages = {57-61},
  doi = {10.11648/j.cbb.20170505.11},
  url = {https://doi.org/10.11648/j.cbb.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20170505.11},
  abstract = {The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 µm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Problems of Bad Contrast in Conventional Microscopy Solution and Speckle Elimination with a Laser Fourier Holographic Microscope
AU  - Vladimir Boris Karpov
Y1  - 2017/11/20
PY  - 2017
N1  - https://doi.org/10.11648/j.cbb.20170505.11
DO  - 10.11648/j.cbb.20170505.11
T2  - Computational Biology and Bioinformatics
JF  - Computational Biology and Bioinformatics
JO  - Computational Biology and Bioinformatics
SP  - 57
EP  - 61
PB  - Science Publishing Group
SN  - 2330-8281
UR  - https://doi.org/10.11648/j.cbb.20170505.11
AB  - The problem of bad contrast in conventional microscopy is well-known and was solved in part by colouring the samples. It is shown theoretically that a laser Fourier holographic microscope produces images undisturbed by speckle-noise. A laser holographic microscope (LHM) is investigated experimentally. The instrument uses visible radiation of λ = 0.514 µm, Mach – Zehnder scheme optical setup, and CCD detector of the hologram. Images are reconstructed digitally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied without any drying as for electron microscope. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-quality Nikon conventional optical microscope with immersion oil and green filter indicates dramatically different contrast. The ultrahigh contrast of the LHM gives much more micromorphological information.
VL  - 5
IS  - 5
ER  -

Copy | Download