Phytochemical Study and Protective effect of <sub>Diospyros lotus</sub> Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats

Gehan Fawzy Abdel Raoof; Khaled Younes Mohamed; Emam Waked; Hesham Anwar Gomaa

doi:doi:10.11648/j.ijbc.20190401.14

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Phytochemical Study and Protective effect of _{Diospyros lotus} Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats

Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa

Published in International Journal of Bioorganic Chemistry (Volume 4, Issue 1)

Received: 8 February 2019 Accepted: 8 March 2019 Published: 30 March 2019

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Abstract

The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total ﬂavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were signiﬁcantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, ﬂavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.

Published in	International Journal of Bioorganic Chemistry (Volume 4, Issue 1)
DOI	10.11648/j.ijbc.20190401.14
Page(s)	19-23
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Carbon Tetrachloride, _{Diospyros Lotus}, Hepatotoprotective, Nephro-Toxicity

References

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Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa. (2019). Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. International Journal of Bioorganic Chemistry, 4(1), 19-23. https://doi.org/10.11648/j.ijbc.20190401.14

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Gehan Fawzy Abdel Raoof; Khaled Younes Mohamed; Emam Waked; Hesham Anwar Gomaa. Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. Int. J. Bioorg. Chem. 2019, 4(1), 19-23. doi: 10.11648/j.ijbc.20190401.14

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AMA Style

Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa. Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. Int J Bioorg Chem. 2019;4(1):19-23. doi: 10.11648/j.ijbc.20190401.14

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@article{10.11648/j.ijbc.20190401.14,
  author = {Gehan Fawzy Abdel Raoof and Khaled Younes Mohamed and Emam Waked and Hesham Anwar Gomaa},
  title = {Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats},
  journal = {International Journal of Bioorganic Chemistry},
  volume = {4},
  number = {1},
  pages = {19-23},
  doi = {10.11648/j.ijbc.20190401.14},
  url = {https://doi.org/10.11648/j.ijbc.20190401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbc.20190401.14},
  abstract = {The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total ﬂavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were signiﬁcantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, ﬂavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.},
 year = {2019}
}

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TY - JOUR
T1 - Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats
AU - Gehan Fawzy Abdel Raoof
AU - Khaled Younes Mohamed
AU - Emam Waked
AU - Hesham Anwar Gomaa
Y1 - 2019/03/30
PY - 2019
N1 - https://doi.org/10.11648/j.ijbc.20190401.14
DO - 10.11648/j.ijbc.20190401.14
T2 - International Journal of Bioorganic Chemistry
JF - International Journal of Bioorganic Chemistry
JO - International Journal of Bioorganic Chemistry
SP - 19
EP - 23
PB - Science Publishing Group
SN - 2578-9392
UR - https://doi.org/10.11648/j.ijbc.20190401.14
AB - The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total ﬂavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were signiﬁcantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, ﬂavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.
VL - 4
IS - 1
ER -

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Author Information

Gehan Fawzy Abdel Raoof

Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt
Khaled Younes Mohamed

Internal Medicine Department, Medical Division, National Research Centre, Giza, Egypt
Emam Waked

Nephrology Department, Theodor Bilharz Research Institute, Cairo, Egypt
Hesham Anwar Gomaa

Biochemistry Department, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt

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APA Style

Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa. (2019). Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. International Journal of Bioorganic Chemistry, 4(1), 19-23. https://doi.org/10.11648/j.ijbc.20190401.14

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Gehan Fawzy Abdel Raoof; Khaled Younes Mohamed; Emam Waked; Hesham Anwar Gomaa. Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. Int. J. Bioorg. Chem. 2019, 4(1), 19-23. doi: 10.11648/j.ijbc.20190401.14

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AMA Style

Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa. Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats. Int J Bioorg Chem. 2019;4(1):19-23. doi: 10.11648/j.ijbc.20190401.14

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@article{10.11648/j.ijbc.20190401.14,
  author = {Gehan Fawzy Abdel Raoof and Khaled Younes Mohamed and Emam Waked and Hesham Anwar Gomaa},
  title = {Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats},
  journal = {International Journal of Bioorganic Chemistry},
  volume = {4},
  number = {1},
  pages = {19-23},
  doi = {10.11648/j.ijbc.20190401.14},
  url = {https://doi.org/10.11648/j.ijbc.20190401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbc.20190401.14},
  abstract = {The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total ﬂavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were signiﬁcantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, ﬂavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.},
 year = {2019}
}

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