Introduction: Rhodanese is a transferase enzyme that catalyses detoxification of cyanide. Cyanide is popular for its presence in a wide variety of food materials that are consumed by animals especially man. In this work, we investigated the partially purified rhodanese from cassava leaves in order to ascertain its suitability for cyanide detoxification in cassava tuber. Crude rhodanense was subjected to 80% (NH4)2SO4 precipitation, the percentage yield and purification fold obtained was 68% and 4 respectively. The degree of substrate preference was Na2S2O3>2-mercaptoethanol>ammonium persulphate>sodium metabisulfite>sodium sulfite. Apparent Km and Vmax were 18.2 mM and 13.9 RU/ml respectively. While that of Na2S2O3 were 10.9 mM and 5.8 RU/ml respectively. The substrate specificity Vmax/Km revealed that KCN was a better substrate. The optimum pH and temperature of rhodanese from cassava leaves were 6.0 and 40°C respectively. Result: The information provided by this study can be exploited for understanding cyanide distribution and detoxification of cassava.
| Published in | International Journal of Bioorganic Chemistry (Volume 6, Issue 2) |
| DOI | 10.11648/j.ijbc.20210602.12 |
| Page(s) | 21-25 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Rhodanese, Cyanogenic Glycosides, Detoxification, Cyanophoric Plant, Manihot esculenta
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APA Style
Omowumi Funke Adedugbe, Oluwatosin Desola Owolala, Olutosin Samuel Ilesanmi. (2021). Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria. International Journal of Bioorganic Chemistry, 6(2), 21-25. https://doi.org/10.11648/j.ijbc.20210602.12
ACS Style
Omowumi Funke Adedugbe; Oluwatosin Desola Owolala; Olutosin Samuel Ilesanmi. Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria. Int. J. Bioorg. Chem. 2021, 6(2), 21-25. doi: 10.11648/j.ijbc.20210602.12
AMA Style
Omowumi Funke Adedugbe, Oluwatosin Desola Owolala, Olutosin Samuel Ilesanmi. Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria. Int J Bioorg Chem. 2021;6(2):21-25. doi: 10.11648/j.ijbc.20210602.12
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Download@article{10.11648/j.ijbc.20210602.12,
author = {Omowumi Funke Adedugbe and Oluwatosin Desola Owolala and Olutosin Samuel Ilesanmi},
title = {Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria},
journal = {International Journal of Bioorganic Chemistry},
volume = {6},
number = {2},
pages = {21-25},
doi = {10.11648/j.ijbc.20210602.12},
url = {https://doi.org/10.11648/j.ijbc.20210602.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbc.20210602.12},
abstract = {Introduction: Rhodanese is a transferase enzyme that catalyses detoxification of cyanide. Cyanide is popular for its presence in a wide variety of food materials that are consumed by animals especially man. In this work, we investigated the partially purified rhodanese from cassava leaves in order to ascertain its suitability for cyanide detoxification in cassava tuber. Crude rhodanense was subjected to 80% (NH4)2SO4 precipitation, the percentage yield and purification fold obtained was 68% and 4 respectively. The degree of substrate preference was Na2S2O3>2-mercaptoethanol>ammonium persulphate>sodium metabisulfite>sodium sulfite. Apparent Km and Vmax were 18.2 mM and 13.9 RU/ml respectively. While that of Na2S2O3 were 10.9 mM and 5.8 RU/ml respectively. The substrate specificity Vmax/Km revealed that KCN was a better substrate. The optimum pH and temperature of rhodanese from cassava leaves were 6.0 and 40°C respectively. Result: The information provided by this study can be exploited for understanding cyanide distribution and detoxification of cassava.},
year = {2021}
}
TY - JOUR T1 - Properties of Partially Purified Rhodanese from Leaves of Cassava in Owo Southwestern Nigeria AU - Omowumi Funke Adedugbe AU - Oluwatosin Desola Owolala AU - Olutosin Samuel Ilesanmi Y1 - 2021/11/19 PY - 2021 N1 - https://doi.org/10.11648/j.ijbc.20210602.12 DO - 10.11648/j.ijbc.20210602.12 T2 - International Journal of Bioorganic Chemistry JF - International Journal of Bioorganic Chemistry JO - International Journal of Bioorganic Chemistry SP - 21 EP - 25 PB - Science Publishing Group SN - 2578-9392 UR - https://doi.org/10.11648/j.ijbc.20210602.12 AB - Introduction: Rhodanese is a transferase enzyme that catalyses detoxification of cyanide. Cyanide is popular for its presence in a wide variety of food materials that are consumed by animals especially man. In this work, we investigated the partially purified rhodanese from cassava leaves in order to ascertain its suitability for cyanide detoxification in cassava tuber. Crude rhodanense was subjected to 80% (NH4)2SO4 precipitation, the percentage yield and purification fold obtained was 68% and 4 respectively. The degree of substrate preference was Na2S2O3>2-mercaptoethanol>ammonium persulphate>sodium metabisulfite>sodium sulfite. Apparent Km and Vmax were 18.2 mM and 13.9 RU/ml respectively. While that of Na2S2O3 were 10.9 mM and 5.8 RU/ml respectively. The substrate specificity Vmax/Km revealed that KCN was a better substrate. The optimum pH and temperature of rhodanese from cassava leaves were 6.0 and 40°C respectively. Result: The information provided by this study can be exploited for understanding cyanide distribution and detoxification of cassava. VL - 6 IS - 2 ER -
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