Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women

Habib Fall; Fatimata Mbaye; Mbacké Sembene

doi:doi:10.11648/j.ijgg.20241204.18

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Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women

Habib Fall^*

, Fatimata Mbaye, Mbacké Sembene

Published in International Journal of Genetics and Genomics (Volume 12, Issue 4)

Received: 15 October 2024 Accepted: 12 November 2024 Published: 29 November 2024

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Abstract

In Senegal, ovarian cancer is the 3rd most common cancer in women with an incidence of 5.0/100,000 women. Thirty-five cancerous tissues, twenty-seven healthy tissues were included in this study. Due to the anatomical position of the ovary, the removal of a sample of suspicious tissue from each patient involves surgery through laparotomy or laparoscopy after obtaining consent. DNA extraction, polymerase chain reaction (PCR) and sequencing were performed to obtain sequences. BioEdit version 7.0.5.3 2005, Harlequin version 3.0, DnaSP version 5.10.01, MEGA 6 were used to perform the analyses. The results show a higher percentage of transition in cancerous tissues (91.45) than in healthy tissues (75.19) in contrast to transversions which are greater in healthy tissues (24.84) than in cancerous tissues (8.54), and the mutation rate (R) is also higher in cancerous tissues (10.712) than in healthy tissues (3.079). Analysis of the polymorphism revealed high values of haplotypic diversity in both cancerous tissues (0.662±0.085) and healthy tissues (0.997±0.011), and low nucleotide diversity values in both tissues (cancerous tissues=0.00922±0.00175; healthy tissues=0.01539±0.00175), these results show us that the genetic evolution of mutations in ovarian cancer has a strong polymorphism. It was also found that the value of the genetic distance between healthy tissues (0.016) was higher than that observed between cancerous tissues (0.009). The genetic distance between healthy and cancerous tissues is 0.015 closer than that observed between healthy tissues. The value of genetic differentiation between healthy and cancerous tissues is significant; this demonstrates a much faster proliferation of cancer cells. The objective of this study is, on the one hand, to better understand the target population by clearly identifying demographic parameters and on the other hand, to evaluate the involvement of somatic mutations and mitochondrial DNA gene expression in the occurrence of ovarian cancer in women in Senegal. The specific objectives are to search for mutations of interest by sequencing mtDNA genes with quasi-maternal inheritance and the impact of these mutations in the D-loop region in healthy and diseased tissues in the patient, but also to learn about the diversity, differentiation and genetic evolution of ovarian cancer in Senegalese women.

Published in	International Journal of Genetics and Genomics (Volume 12, Issue 4)
DOI	10.11648/j.ijgg.20241204.18
Page(s)	127-135
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cancer, Ovary, Mutations, Epidemiology, D-loop, Senegal

References

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APA Style

Fall, H., Mbaye, F., Sembene, M. (2024). Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. International Journal of Genetics and Genomics, 12(4), 127-135. https://doi.org/10.11648/j.ijgg.20241204.18

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ACS Style

Fall, H.; Mbaye, F.; Sembene, M. Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. Int. J. Genet. Genomics 2024, 12(4), 127-135. doi: 10.11648/j.ijgg.20241204.18

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AMA Style

Fall H, Mbaye F, Sembene M. Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. Int J Genet Genomics. 2024;12(4):127-135. doi: 10.11648/j.ijgg.20241204.18

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@article{10.11648/j.ijgg.20241204.18,
  author = {Habib Fall and Fatimata Mbaye and Mbacké Sembene},
  title = {Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women
},
  journal = {International Journal of Genetics and Genomics},
  volume = {12},
  number = {4},
  pages = {127-135},
  doi = {10.11648/j.ijgg.20241204.18},
  url = {https://doi.org/10.11648/j.ijgg.20241204.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20241204.18},
  abstract = {In Senegal, ovarian cancer is the 3rd most common cancer in women with an incidence of 5.0/100,000 women. Thirty-five cancerous tissues, twenty-seven healthy tissues were included in this study. Due to the anatomical position of the ovary, the removal of a sample of suspicious tissue from each patient involves surgery through laparotomy or laparoscopy after obtaining consent. DNA extraction, polymerase chain reaction (PCR) and sequencing were performed to obtain sequences. BioEdit version 7.0.5.3 2005, Harlequin version 3.0, DnaSP version 5.10.01, MEGA 6 were used to perform the analyses. The results show a higher percentage of transition in cancerous tissues (91.45) than in healthy tissues (75.19) in contrast to transversions which are greater in healthy tissues (24.84) than in cancerous tissues (8.54), and the mutation rate (R) is also higher in cancerous tissues (10.712) than in healthy tissues (3.079). Analysis of the polymorphism revealed high values of haplotypic diversity in both cancerous tissues (0.662±0.085) and healthy tissues (0.997±0.011), and low nucleotide diversity values in both tissues (cancerous tissues=0.00922±0.00175; healthy tissues=0.01539±0.00175), these results show us that the genetic evolution of mutations in ovarian cancer has a strong polymorphism. It was also found that the value of the genetic distance between healthy tissues (0.016) was higher than that observed between cancerous tissues (0.009). The genetic distance between healthy and cancerous tissues is 0.015 closer than that observed between healthy tissues. The value of genetic differentiation between healthy and cancerous tissues is significant; this demonstrates a much faster proliferation of cancer cells. The objective of this study is, on the one hand, to better understand the target population by clearly identifying demographic parameters and on the other hand, to evaluate the involvement of somatic mutations and mitochondrial DNA gene expression in the occurrence of ovarian cancer in women in Senegal. The specific objectives are to search for mutations of interest by sequencing mtDNA genes with quasi-maternal inheritance and the impact of these mutations in the D-loop region in healthy and diseased tissues in the patient, but also to learn about the diversity, differentiation and genetic evolution of ovarian cancer in Senegalese women.
},
 year = {2024}
}

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TY  - JOUR
T1  - Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women

AU  - Habib Fall
AU  - Fatimata Mbaye
AU  - Mbacké Sembene
Y1  - 2024/11/29
PY  - 2024
N1  - https://doi.org/10.11648/j.ijgg.20241204.18
DO  - 10.11648/j.ijgg.20241204.18
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 127
EP  - 135
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20241204.18
AB  - In Senegal, ovarian cancer is the 3rd most common cancer in women with an incidence of 5.0/100,000 women. Thirty-five cancerous tissues, twenty-seven healthy tissues were included in this study. Due to the anatomical position of the ovary, the removal of a sample of suspicious tissue from each patient involves surgery through laparotomy or laparoscopy after obtaining consent. DNA extraction, polymerase chain reaction (PCR) and sequencing were performed to obtain sequences. BioEdit version 7.0.5.3 2005, Harlequin version 3.0, DnaSP version 5.10.01, MEGA 6 were used to perform the analyses. The results show a higher percentage of transition in cancerous tissues (91.45) than in healthy tissues (75.19) in contrast to transversions which are greater in healthy tissues (24.84) than in cancerous tissues (8.54), and the mutation rate (R) is also higher in cancerous tissues (10.712) than in healthy tissues (3.079). Analysis of the polymorphism revealed high values of haplotypic diversity in both cancerous tissues (0.662±0.085) and healthy tissues (0.997±0.011), and low nucleotide diversity values in both tissues (cancerous tissues=0.00922±0.00175; healthy tissues=0.01539±0.00175), these results show us that the genetic evolution of mutations in ovarian cancer has a strong polymorphism. It was also found that the value of the genetic distance between healthy tissues (0.016) was higher than that observed between cancerous tissues (0.009). The genetic distance between healthy and cancerous tissues is 0.015 closer than that observed between healthy tissues. The value of genetic differentiation between healthy and cancerous tissues is significant; this demonstrates a much faster proliferation of cancer cells. The objective of this study is, on the one hand, to better understand the target population by clearly identifying demographic parameters and on the other hand, to evaluate the involvement of somatic mutations and mitochondrial DNA gene expression in the occurrence of ovarian cancer in women in Senegal. The specific objectives are to search for mutations of interest by sequencing mtDNA genes with quasi-maternal inheritance and the impact of these mutations in the D-loop region in healthy and diseased tissues in the patient, but also to learn about the diversity, differentiation and genetic evolution of ovarian cancer in Senegalese women.

VL  - 12
IS  - 4
ER  -

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Author Information

Habib Fall

Genomics Laboratory, Department of Animal Biology, Faculty of Science and Technology, Cheikh Anta Diop University, Dakar, Senegal

Contact Email

http://orcid.org/0009-0008-5457-5033
Fatimata Mbaye

Genomics Laboratory, Department of Animal Biology, Faculty of Science and Technology, Cheikh Anta Diop University, Dakar, Senegal

Contact Email
Mbacké Sembene

Genomics Laboratory, Department of Animal Biology, Faculty of Science and Technology, Cheikh Anta Diop University, Dakar, Senegal

Contact Email

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Plain Text BibTeX RIS

APA Style

Fall, H., Mbaye, F., Sembene, M. (2024). Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. International Journal of Genetics and Genomics, 12(4), 127-135. https://doi.org/10.11648/j.ijgg.20241204.18

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ACS Style

Fall, H.; Mbaye, F.; Sembene, M. Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. Int. J. Genet. Genomics 2024, 12(4), 127-135. doi: 10.11648/j.ijgg.20241204.18

Copy | Download

AMA Style

Fall H, Mbaye F, Sembene M. Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women. Int J Genet Genomics. 2024;12(4):127-135. doi: 10.11648/j.ijgg.20241204.18

Copy | Download

@article{10.11648/j.ijgg.20241204.18,
  author = {Habib Fall and Fatimata Mbaye and Mbacké Sembene},
  title = {Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women
},
  journal = {International Journal of Genetics and Genomics},
  volume = {12},
  number = {4},
  pages = {127-135},
  doi = {10.11648/j.ijgg.20241204.18},
  url = {https://doi.org/10.11648/j.ijgg.20241204.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20241204.18},
  abstract = {In Senegal, ovarian cancer is the 3rd most common cancer in women with an incidence of 5.0/100,000 women. Thirty-five cancerous tissues, twenty-seven healthy tissues were included in this study. Due to the anatomical position of the ovary, the removal of a sample of suspicious tissue from each patient involves surgery through laparotomy or laparoscopy after obtaining consent. DNA extraction, polymerase chain reaction (PCR) and sequencing were performed to obtain sequences. BioEdit version 7.0.5.3 2005, Harlequin version 3.0, DnaSP version 5.10.01, MEGA 6 were used to perform the analyses. The results show a higher percentage of transition in cancerous tissues (91.45) than in healthy tissues (75.19) in contrast to transversions which are greater in healthy tissues (24.84) than in cancerous tissues (8.54), and the mutation rate (R) is also higher in cancerous tissues (10.712) than in healthy tissues (3.079). Analysis of the polymorphism revealed high values of haplotypic diversity in both cancerous tissues (0.662±0.085) and healthy tissues (0.997±0.011), and low nucleotide diversity values in both tissues (cancerous tissues=0.00922±0.00175; healthy tissues=0.01539±0.00175), these results show us that the genetic evolution of mutations in ovarian cancer has a strong polymorphism. It was also found that the value of the genetic distance between healthy tissues (0.016) was higher than that observed between cancerous tissues (0.009). The genetic distance between healthy and cancerous tissues is 0.015 closer than that observed between healthy tissues. The value of genetic differentiation between healthy and cancerous tissues is significant; this demonstrates a much faster proliferation of cancer cells. The objective of this study is, on the one hand, to better understand the target population by clearly identifying demographic parameters and on the other hand, to evaluate the involvement of somatic mutations and mitochondrial DNA gene expression in the occurrence of ovarian cancer in women in Senegal. The specific objectives are to search for mutations of interest by sequencing mtDNA genes with quasi-maternal inheritance and the impact of these mutations in the D-loop region in healthy and diseased tissues in the patient, but also to learn about the diversity, differentiation and genetic evolution of ovarian cancer in Senegalese women.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Impact of Mutations in the D-loop Region in Ovarian Cancer in Senegalese Women

AU  - Habib Fall
AU  - Fatimata Mbaye
AU  - Mbacké Sembene
Y1  - 2024/11/29
PY  - 2024
N1  - https://doi.org/10.11648/j.ijgg.20241204.18
DO  - 10.11648/j.ijgg.20241204.18
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 127
EP  - 135
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20241204.18
AB  - In Senegal, ovarian cancer is the 3rd most common cancer in women with an incidence of 5.0/100,000 women. Thirty-five cancerous tissues, twenty-seven healthy tissues were included in this study. Due to the anatomical position of the ovary, the removal of a sample of suspicious tissue from each patient involves surgery through laparotomy or laparoscopy after obtaining consent. DNA extraction, polymerase chain reaction (PCR) and sequencing were performed to obtain sequences. BioEdit version 7.0.5.3 2005, Harlequin version 3.0, DnaSP version 5.10.01, MEGA 6 were used to perform the analyses. The results show a higher percentage of transition in cancerous tissues (91.45) than in healthy tissues (75.19) in contrast to transversions which are greater in healthy tissues (24.84) than in cancerous tissues (8.54), and the mutation rate (R) is also higher in cancerous tissues (10.712) than in healthy tissues (3.079). Analysis of the polymorphism revealed high values of haplotypic diversity in both cancerous tissues (0.662±0.085) and healthy tissues (0.997±0.011), and low nucleotide diversity values in both tissues (cancerous tissues=0.00922±0.00175; healthy tissues=0.01539±0.00175), these results show us that the genetic evolution of mutations in ovarian cancer has a strong polymorphism. It was also found that the value of the genetic distance between healthy tissues (0.016) was higher than that observed between cancerous tissues (0.009). The genetic distance between healthy and cancerous tissues is 0.015 closer than that observed between healthy tissues. The value of genetic differentiation between healthy and cancerous tissues is significant; this demonstrates a much faster proliferation of cancer cells. The objective of this study is, on the one hand, to better understand the target population by clearly identifying demographic parameters and on the other hand, to evaluate the involvement of somatic mutations and mitochondrial DNA gene expression in the occurrence of ovarian cancer in women in Senegal. The specific objectives are to search for mutations of interest by sequencing mtDNA genes with quasi-maternal inheritance and the impact of these mutations in the D-loop region in healthy and diseased tissues in the patient, but also to learn about the diversity, differentiation and genetic evolution of ovarian cancer in Senegalese women.

VL  - 12
IS  - 4
ER  -

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