Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics

Bijo Elsy; Aijaz Ahmed Khan; Veena Maheshwari

doi:doi:10.11648/j.ijnfs.20180702.12

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Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari

Published in International Journal of Nutrition and Food Sciences (Volume 7, Issue 2)

Received: 22 January 2018 Accepted: 5 February 2018 Published: 27 February 2018

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Abstract

In diabetes the structural and functional recovery of skeletal muscle is impaired due to persistent hyperglycemia-induced oxidative stress. Vitamin E is known to be essential antioxidant for maintains the skeletal muscle homeostasis thus preventing oxidative damages. This study is designed to explore the effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on crushed muscle regeneration in both healthy and diabetic rats. Diabetes was induced through single subcutaneous injection of aqueous alloxan at the dose of 100 mg/kg. Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each, orally, daily for three weeks. Through a horizontal mid-thigh skin incision and splitting of the fascia gluteus maximus was approached and crushed with Kocher’s forceps. Skin wound was closed with an absorbable suture. The crush-induced degenerative and regenerative changes in the muscle were studied by assessing the histological features, histomorphological measurements and biochemical analyses at the end of 3^rd weeks. One- way ‘ANOVA’ and Student’s t-test were used for statistical analysis. All results revealed that the vitamin E isoforms have potency to maintain glycemic level, improve the antioxidant capacity and hasten the process of regeneration, revascularization, reinnervation and connective tissue remodeling in skeletal muscle after crush injury. It is therefore, concluded that the vitamin E isoforms are useful nutritional supplements for skeletal muscle functional and structural recovery in both healthy and diabetics.

Published in	International Journal of Nutrition and Food Sciences (Volume 7, Issue 2)
DOI	10.11648/j.ijnfs.20180702.12
Page(s)	47-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Antioxidant, Crush-Injury, d-α-tocopherol, d-δ-tocotrienol Rich Fraction, Diabetes, Rats, Skeletal Muscle

References

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APA Style

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. (2018). Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. International Journal of Nutrition and Food Sciences, 7(2), 47-55. https://doi.org/10.11648/j.ijnfs.20180702.12

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ACS Style

Bijo Elsy; Aijaz Ahmed Khan; Veena Maheshwari. Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. Int. J. Nutr. Food Sci. 2018, 7(2), 47-55. doi: 10.11648/j.ijnfs.20180702.12

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AMA Style

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. Int J Nutr Food Sci. 2018;7(2):47-55. doi: 10.11648/j.ijnfs.20180702.12

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@article{10.11648/j.ijnfs.20180702.12,
  author = {Bijo Elsy and Aijaz Ahmed Khan and Veena Maheshwari},
  title = {Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {7},
  number = {2},
  pages = {47-55},
  doi = {10.11648/j.ijnfs.20180702.12},
  url = {https://doi.org/10.11648/j.ijnfs.20180702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20180702.12},
  abstract = {In diabetes the structural and functional recovery of skeletal muscle is impaired due to persistent hyperglycemia-induced oxidative stress. Vitamin E is known to be essential antioxidant for maintains the skeletal muscle homeostasis thus preventing oxidative damages. This study is designed to explore the effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on crushed muscle regeneration in both healthy and diabetic rats. Diabetes was induced through single subcutaneous injection of aqueous alloxan at the dose of 100 mg/kg. Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each, orally, daily for three weeks. Through a horizontal mid-thigh skin incision and splitting of the fascia gluteus maximus was approached and crushed with Kocher’s forceps. Skin wound was closed with an absorbable suture. The crush-induced degenerative and regenerative changes in the muscle were studied by assessing the histological features, histomorphological measurements and biochemical analyses at the end of 3rd weeks. One- way ‘ANOVA’ and Student’s t-test were used for statistical analysis. All results revealed that the vitamin E isoforms have potency to maintain glycemic level, improve the antioxidant capacity and hasten the process of regeneration, revascularization, reinnervation and connective tissue remodeling in skeletal muscle after crush injury. It is therefore, concluded that the vitamin E isoforms are useful nutritional supplements for skeletal muscle functional and structural recovery in both healthy and diabetics.},
 year = {2018}
}

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TY - JOUR
T1 - Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics
AU - Bijo Elsy
AU - Aijaz Ahmed Khan
AU - Veena Maheshwari
Y1 - 2018/02/27
PY - 2018
N1 - https://doi.org/10.11648/j.ijnfs.20180702.12
DO - 10.11648/j.ijnfs.20180702.12
T2 - International Journal of Nutrition and Food Sciences
JF - International Journal of Nutrition and Food Sciences
JO - International Journal of Nutrition and Food Sciences
SP - 47
EP - 55
PB - Science Publishing Group
SN - 2327-2716
UR - https://doi.org/10.11648/j.ijnfs.20180702.12
AB - In diabetes the structural and functional recovery of skeletal muscle is impaired due to persistent hyperglycemia-induced oxidative stress. Vitamin E is known to be essential antioxidant for maintains the skeletal muscle homeostasis thus preventing oxidative damages. This study is designed to explore the effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on crushed muscle regeneration in both healthy and diabetic rats. Diabetes was induced through single subcutaneous injection of aqueous alloxan at the dose of 100 mg/kg. Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each, orally, daily for three weeks. Through a horizontal mid-thigh skin incision and splitting of the fascia gluteus maximus was approached and crushed with Kocher’s forceps. Skin wound was closed with an absorbable suture. The crush-induced degenerative and regenerative changes in the muscle were studied by assessing the histological features, histomorphological measurements and biochemical analyses at the end of 3rd weeks. One- way ‘ANOVA’ and Student’s t-test were used for statistical analysis. All results revealed that the vitamin E isoforms have potency to maintain glycemic level, improve the antioxidant capacity and hasten the process of regeneration, revascularization, reinnervation and connective tissue remodeling in skeletal muscle after crush injury. It is therefore, concluded that the vitamin E isoforms are useful nutritional supplements for skeletal muscle functional and structural recovery in both healthy and diabetics.
VL - 7
IS - 2
ER -

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Author Information

Bijo Elsy

Department of Anatomy, JN Medical College, Aligarh Muslim University, Aligarh, India
Aijaz Ahmed Khan

Department of Anatomy, JN Medical College, Aligarh Muslim University, Aligarh, India
Veena Maheshwari

Department of Pathology, JN Medical College, Aligarh Muslim University, Aligarh, India

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Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. (2018). Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. International Journal of Nutrition and Food Sciences, 7(2), 47-55. https://doi.org/10.11648/j.ijnfs.20180702.12

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ACS Style

Bijo Elsy; Aijaz Ahmed Khan; Veena Maheshwari. Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. Int. J. Nutr. Food Sci. 2018, 7(2), 47-55. doi: 10.11648/j.ijnfs.20180702.12

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AMA Style

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari. Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics. Int J Nutr Food Sci. 2018;7(2):47-55. doi: 10.11648/j.ijnfs.20180702.12

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@article{10.11648/j.ijnfs.20180702.12,
  author = {Bijo Elsy and Aijaz Ahmed Khan and Veena Maheshwari},
  title = {Co-Administered Vitamin E Isoforms d-α-tocopherol and d-δ-tocotrienol Rich Fraction Promote Regeneration of Skeletal Muscle in Diabetics},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {7},
  number = {2},
  pages = {47-55},
  doi = {10.11648/j.ijnfs.20180702.12},
  url = {https://doi.org/10.11648/j.ijnfs.20180702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20180702.12},
  abstract = {In diabetes the structural and functional recovery of skeletal muscle is impaired due to persistent hyperglycemia-induced oxidative stress. Vitamin E is known to be essential antioxidant for maintains the skeletal muscle homeostasis thus preventing oxidative damages. This study is designed to explore the effect of d-α-tocopherol and d-δ-tocotrienol rich fraction (d-δ-TRF) on crushed muscle regeneration in both healthy and diabetic rats. Diabetes was induced through single subcutaneous injection of aqueous alloxan at the dose of 100 mg/kg. Twenty four albino rats were divided into four groups; healthy control, diabetic control, healthy treated and diabetic treated. Treated groups received 100 mg/kg of d-α-tocopherol and d-δ-TRF each, orally, daily for three weeks. Through a horizontal mid-thigh skin incision and splitting of the fascia gluteus maximus was approached and crushed with Kocher’s forceps. Skin wound was closed with an absorbable suture. The crush-induced degenerative and regenerative changes in the muscle were studied by assessing the histological features, histomorphological measurements and biochemical analyses at the end of 3rd weeks. One- way ‘ANOVA’ and Student’s t-test were used for statistical analysis. All results revealed that the vitamin E isoforms have potency to maintain glycemic level, improve the antioxidant capacity and hasten the process of regeneration, revascularization, reinnervation and connective tissue remodeling in skeletal muscle after crush injury. It is therefore, concluded that the vitamin E isoforms are useful nutritional supplements for skeletal muscle functional and structural recovery in both healthy and diabetics.},
 year = {2018}
}

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