Research Article | | Peer-Reviewed

Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil

Received: 17 May 2024     Accepted: 11 June 2024     Published: 6 August 2024
Views:       Downloads:
Abstract

Rice bran oil was extracted by product of milling of Oryza sativa seeds. The focus of the study was to determine the physiochemical, phytochemicals, micronutrients and antioxidant potentiality of rice bran oil and effect of refining process. The oil is extracted from bran of three popular rice varieties (BRRI-28, BRRI-29, Rajvogh) of Bangladesh. The acid value, peroxide value, iodine value and saponification value of extracted oils were observed in the range of 14.03-16.82 mgKOH/g, 26.68-34.50 meqO2/kg, 71.61-84.70 gI2/100g and 175.13-180.26 mgKOH/g respectively. Atomic absorption spectrophotometric method was used for determination of micronutrients. The concentrations for copper, zinc, iron and chromium were found to comprise between 0.03-0.25, 0.25-0.39, 17.97-64.47 and 0.96-4.87 ppm respectively. It was observed that BRRI-29 showed the greatest DPPH radical scavenging activity with the IC50 value of 187.55µg/ml, followed by BRRI-28 (IC50- 284.04), Rajvogh (IC50- 325.56) and refined oil (IC50- 465.12). All the samples acted upon nitric oxide free radical according to following order- BRRI-29 (IC50- 286.4)> BRRI-28 (IC50 -416.81) >Rajvogh (IC50 -453.95)> refined oil (IC50- 557.86).All the extracted and refined oils were found to have considerably good antioxidant activity in a dose dependent manner. The extracted oils were found to contain more phenolic and flavonoid content than refined oil that serve as antioxidants. Some literatures show that, RBO contains tocotrienol and oryzanol which absent in soybean oil which regarded to be the best in perspective of antioxidant potentiality and categorize rice bran oil as promising edible oil. Comparative to crude bran oil commercial refined oil were lower in these parameters which shows the effect of refining process. Further investigation should be conducted on animal model (in vivo study) for safe human consumption. The knowledge would be useful in recommending various ways of using the oil in food industry, as well as in households.

Published in International Journal of Nutrition and Food Sciences (Volume 13, Issue 4)
DOI 10.11648/j.ijnfs.20241304.13
Page(s) 147-157
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rice Bran Oil, Refined Oil, Phytochemicals, Antioxidant, Micronutrients

References
[1] T. B. Salam, B. Karmakar, S. M. T. Hossain, M. H. Robin, Z. Mariam, M. Hossain, Agronomic Performance of Modern Rice Varieties in South-west Bangladesh, Plant Science Today 6(4) (2019) 528-532,
[2] C. A. garcia, G. Gavino, M. B. Mosqueda, P. Hevia, V. C. Gavino, Correlation of tocopherol, tocotrienol, γ-oryzanol and total polyphenol content in rice bran with different antioxidant capacity assays,” Food Chem. 102(4) (2007) 1228-1232,
[3] A. Rosniyana, M. A. Hashifah, S. Norin, The physico-chemical properties and nutritional composition of rice bran produced at different milling degrees of rice, J. Trop. Agri. and Food Sci. 35(1) (2007) 99-105.
[4] M. Patel, S. N. Naik, Gamma-Oryzanol from rice bran oil-A review, J. Sci. Ind. Res. 63(2004) 569-578.
[5] T. A. Wilson, H. M. Idreis, C. M. Taylor, R. J. Nicolosi, Whole fat rice bran reduces the development of early aortic atherosclerosis in hypercholesterolemic hamsters compared with wheat bran, Nutri Res. 22(11) (2002) 1319-1332,
[6] M. Ghosh, Review on Recent Trends in Rice Bran Oil Processing, J. American Oil Chem. Soci. 84(4) (2007) 315-324,
[7] V. V. Hoed, G. Depaemelaere, J. V. Ayala, P. Santiwattana, R. Verhe, W. De Greyt, Influence of Chemical Refining on the Major and Minor Components of Rice Bran Oil, J. American Oil Chem. Soci. 83(2006) 315-321,
[8] [AOAC]. Association of Official Analytical Chemistry International. (1990). Official methods of analysis of AOAC international. 15th ed. Methods 932.06, 925.09, 985.29, 923.03.
[9] T. Osawa, M. Namiki, A Novel Type of Antioxidant Isolated from Leaf Wax of Eucalyptus leaves, Agri. Bio. Chem. 45(3) (1981) 735-739,
[10] U. Salma, H. Shahjalal, T. Ahmmed, M. U. Ansary, I. Khalil, N. Karim, Effect of Boiling on the Nutritional Value, Phytochemical Contents, and Antioxidant Activity of Commonly Consumed Potato ( Solanum tuberosum L.) Varieties in Bangladesh, J. Food Nutr Res. 8(8) (2020) 382–391,
[11] L. L. Mensor, F. S. Menezes, G. G. Leitao, A. S. Reis, T. C. dos Santos, C. S. Coube, S. G. Leitao, Screening of Brazilian Plant Extracts for Antioxidant Activity by the Use of DPPH Free Radical Method, Phytotherapy Research 15(2001) 127-130,
[12] D. C. Garratt, The Quantatitive Analysis of Drugs, Springer, Boston, MA (1964).
[13] F. Anwar, T. G. Kazi, R. Saleem, M. I. Bhanger, Rapid determination of some trace metals in several oils and fats, Grasas y Aceites 55(2) (2004) 160-168,
[14] I. E. Akubugwo, A. E Ugbogu, Physicochemical studies on oils from five selected Nigerian plant seeds, Pakistan J. Nutri. 6(1) (2007) 75-78,
[15] R. Kusum, H. Bommayya, P. P Fayaz, H. D. Ramachandran, Palm oil and rice bran oil : Current status and future prospects, Int. J. Plant Physiol. Biochem. 3(8), (2011) 125-132,
[16] O. Pourali, F. S. Asghari, H. Yoshida, A Rapid and Ecofriendly Treatment Technique for Rice Bran Oil Stabilization and Extraction under Sub-critical Water Condition, Proc. World Cong. Eng. Comp. Sci. 1(2009) 20-22.
[17] H. Ulu, Evaluation of three 2-thiobarbituric acid methods for the measurement of lipid oxidation in various meats and meat products, Meat Sci. 67(4) (2004) 683-687,
[18] B. S. Ogunsina, A. S. Bhatnagar, T. N. Indira, C. Radha, Proximate composition of African bush mango ( Irvingia gabonesis ) kernels and characteristics of its oil, Ife J. Sci. 14(1) (2012) 177-183.
[19] H. Lawson, Physical properties in Food oil and Fats: Technology, Utilization and Nutrition. Chapman and Hall, New York (1995) 28-38,
[20] C. P Tan, Y. B Che Man, S Jinap, M. S. A Yusoff, Effects of microwave heating on the quality characteristics and thermal properties of RBD palm olein, Innovative Food Science & Emerging Technologies, 3(2) (2002) 157-163,
[21] G. O. Oyeleke, O. Afolabi, O. A. Olayiwola, R. O Adetoro, Oil Quality Characteristics and Effects of Temperature Variations on Some Functional Properties of Horse Eye (Dioclea reflexa) Seed Flour, IOSR J. Env. Sci. Toxi. Food Tech. 2(2) (2012) 38-42.
[22] K. S. Sim, A. M. Sri Nurestri, A. W Norhnom, Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts, Pharmacogn. Mag. 6(23) (2010) 248-254,
[23] B. D. Oomah, E. Kenaschuk, G. Mazzat, Phenolic Acids in Flaxseed, J. Agric. Food Chem. 43(1995) 2016-2019,
[24] H. S. Gujral, P. Sharma, N. Gupta, A. A. Wani, Antioxidant properties of legumes and their morphological fractions as affected by cooking, Food Sci. Biotech. 22(1), (2013) 187-194,
[25] M. Sardarodiyan, E. A. Salehi, Bioactive phytochemicals in Rice Bran : processing and functional properties, Int. J. Pharma Tech Res. 9(6) (2016) 401-408.
[26] P. Kalaisezhiyen and S. Vadivukkarasi, Evaluation of Free Radical Scavenging Activity of Various Leaf Extracts from Kedrostis foetidissima (Jacq.) Cogn., Food Sci. Human Well. 4(1) (2015) 42-46,
[27] J. Chandran, D. R. S. Kumar, M. V. Reshma, P. Jayamurthy, A. Sundaresan, P. Nisha, Comparative Study On The Total Phenolic Content And Radical Scavenging Activity Of Common Edible Vegetable Oils, J. Food Biochem. 38(1) (2013) 38-49,
[28] A. Malik, A. Kushnoor, V. Saini, S. Singhal, S. Kumar, Y. C. Yadav, In Vitro Antioxidant Proprties of Scopoletin, Pharmacologyonline 1(2011) 1125-1133.
[29] S. Vorarat, C. Managit, L. Iamthanakul, W. Soparat, N. Kamkaen, Examination of Antioxidant Activity and Development of Rice Bran Oiland Gammma- Oryzanol Microemulsion, J. Health Res. 24(2) (2010) 67-72.
[30] S. Jan, M. R. Khan, U. Rashid, J. Bokhari, Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit, Osong Pub. Health Res Pers. 4(5) (2013) 246-254,
[31] V. Yermilov, J. Rubio, H. Ohshima, Formation of 8-nitroguanine in DNA treated with peroxynitrite in vitro and its rapid removal from DNA by depurination, FEBS Lett. 376(3) (1995) 207-210,
[32] Smouse, T. H., Factors Affecting Oil Quality and Stability in Methods to Assess Oil Quality and Stability of Oils and Fat-Containing Foods, AOCS Publishing, (1995) 17-36.
[33] R. Ansari, T. G. Kazi, M. K. Jamali, M. B. Arain, M. D. Wagan, N. Jalbani, H. I. Afridi, A. Q. Shah, Variation in accumulation of heavy metals in different verities of sunflower seed oil with the aid of multivariate technique, Food Chem. 115(1) (2009) 318-323,
[34] E. Pehlivan, G. Arslan, F. Gode, T. Altun, M. M. Ozcan, Determination of some inorganic metals in edible vegetable oils by inductively coupled plasma atomic emission spectroscopy (ICP-AES), GRASAS Y ACEITES 59(3) (2008) 239-244,
[35] S. Islam, K. Ahmed, H. A. Mamun, Heavy Metals in Cereals and Pulses: Health Implications in Bangladesh, J. Agric. Food Chem. 62(44) (2014) 10828-10835,
Cite This Article
  • APA Style

    Afrose, S., Salma, U., Ahmmed, T., Sohel, M., Khatun, M. A. (2024). Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil. International Journal of Nutrition and Food Sciences, 13(4), 147-157. https://doi.org/10.11648/j.ijnfs.20241304.13

    Copy | Download

    ACS Style

    Afrose, S.; Salma, U.; Ahmmed, T.; Sohel, M.; Khatun, M. A. Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil. Int. J. Nutr. Food Sci. 2024, 13(4), 147-157. doi: 10.11648/j.ijnfs.20241304.13

    Copy | Download

    AMA Style

    Afrose S, Salma U, Ahmmed T, Sohel M, Khatun MA. Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil. Int J Nutr Food Sci. 2024;13(4):147-157. doi: 10.11648/j.ijnfs.20241304.13

    Copy | Download

  • @article{10.11648/j.ijnfs.20241304.13,
      author = {Sanjida Afrose and Umme Salma and Tanvir Ahmmed and Md. Sohel and Mst. Afifa Khatun},
      title = {Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil
    },
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {13},
      number = {4},
      pages = {147-157},
      doi = {10.11648/j.ijnfs.20241304.13},
      url = {https://doi.org/10.11648/j.ijnfs.20241304.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20241304.13},
      abstract = {Rice bran oil was extracted by product of milling of Oryza sativa seeds. The focus of the study was to determine the physiochemical, phytochemicals, micronutrients and antioxidant potentiality of rice bran oil and effect of refining process. The oil is extracted from bran of three popular rice varieties (BRRI-28, BRRI-29, Rajvogh) of Bangladesh. The acid value, peroxide value, iodine value and saponification value of extracted oils were observed in the range of 14.03-16.82 mgKOH/g, 26.68-34.50 meqO2/kg, 71.61-84.70 gI2/100g and 175.13-180.26 mgKOH/g respectively. Atomic absorption spectrophotometric method was used for determination of micronutrients. The concentrations for copper, zinc, iron and chromium were found to comprise between 0.03-0.25, 0.25-0.39, 17.97-64.47 and 0.96-4.87 ppm respectively. It was observed that BRRI-29 showed the greatest DPPH radical scavenging activity with the IC50 value of 187.55µg/ml, followed by BRRI-28 (IC50- 284.04), Rajvogh (IC50- 325.56) and refined oil (IC50- 465.12). All the samples acted upon nitric oxide free radical according to following order- BRRI-29 (IC50- 286.4)> BRRI-28 (IC50 -416.81) >Rajvogh (IC50 -453.95)> refined oil (IC50- 557.86).All the extracted and refined oils were found to have considerably good antioxidant activity in a dose dependent manner. The extracted oils were found to contain more phenolic and flavonoid content than refined oil that serve as antioxidants. Some literatures show that, RBO contains tocotrienol and oryzanol which absent in soybean oil which regarded to be the best in perspective of antioxidant potentiality and categorize rice bran oil as promising edible oil. Comparative to crude bran oil commercial refined oil were lower in these parameters which shows the effect of refining process. Further investigation should be conducted on animal model (in vivo study) for safe human consumption. The knowledge would be useful in recommending various ways of using the oil in food industry, as well as in households.
    },
     year = {2024}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Evaluation of Physiochemical, Phytochemicals, Antioxidant, Micronutrients Properties of Bangladeshi Crude and Commercial Rice Bran Oil
    
    AU  - Sanjida Afrose
    AU  - Umme Salma
    AU  - Tanvir Ahmmed
    AU  - Md. Sohel
    AU  - Mst. Afifa Khatun
    Y1  - 2024/08/06
    PY  - 2024
    N1  - https://doi.org/10.11648/j.ijnfs.20241304.13
    DO  - 10.11648/j.ijnfs.20241304.13
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 147
    EP  - 157
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20241304.13
    AB  - Rice bran oil was extracted by product of milling of Oryza sativa seeds. The focus of the study was to determine the physiochemical, phytochemicals, micronutrients and antioxidant potentiality of rice bran oil and effect of refining process. The oil is extracted from bran of three popular rice varieties (BRRI-28, BRRI-29, Rajvogh) of Bangladesh. The acid value, peroxide value, iodine value and saponification value of extracted oils were observed in the range of 14.03-16.82 mgKOH/g, 26.68-34.50 meqO2/kg, 71.61-84.70 gI2/100g and 175.13-180.26 mgKOH/g respectively. Atomic absorption spectrophotometric method was used for determination of micronutrients. The concentrations for copper, zinc, iron and chromium were found to comprise between 0.03-0.25, 0.25-0.39, 17.97-64.47 and 0.96-4.87 ppm respectively. It was observed that BRRI-29 showed the greatest DPPH radical scavenging activity with the IC50 value of 187.55µg/ml, followed by BRRI-28 (IC50- 284.04), Rajvogh (IC50- 325.56) and refined oil (IC50- 465.12). All the samples acted upon nitric oxide free radical according to following order- BRRI-29 (IC50- 286.4)> BRRI-28 (IC50 -416.81) >Rajvogh (IC50 -453.95)> refined oil (IC50- 557.86).All the extracted and refined oils were found to have considerably good antioxidant activity in a dose dependent manner. The extracted oils were found to contain more phenolic and flavonoid content than refined oil that serve as antioxidants. Some literatures show that, RBO contains tocotrienol and oryzanol which absent in soybean oil which regarded to be the best in perspective of antioxidant potentiality and categorize rice bran oil as promising edible oil. Comparative to crude bran oil commercial refined oil were lower in these parameters which shows the effect of refining process. Further investigation should be conducted on animal model (in vivo study) for safe human consumption. The knowledge would be useful in recommending various ways of using the oil in food industry, as well as in households.
    
    VL  - 13
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka, Bangladesh

  • Department of Biochemistry and Molecular Biology, Primeasia University, Dhaka, Bangladesh

  • Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka, Bangladesh

  • Department of Biochemistry and Molecular Biology, Primeasia University, Dhaka, Bangladesh

  • Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Dhaka, Bangladesh

  • Sections