<i>In vitro</i> Antioxidant, Anti-inflammatory and Antimicrobial Activities of <i>Garcinia kola</i> Seeds (Clusiaceae)

Affo Dermane; Sandra N’samba Gbati; Batomayena Bakoma

doi:doi:10.11648/j.ijpc.20210706.11

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In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae)

Affo Dermane, Sandra N’samba Gbati, Batomayena Bakoma

Published in International Journal of Pharmacy and Chemistry (Volume 7, Issue 6)

Received: 3 November 2021 Accepted: 19 November 2021 Published: 25 November 2021

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Abstract

The use of plants in health promotion is increasingly noteworthy in the face of the re-emergence of diseases and the limitation of conventional drugs. Garcinia kola (Clusiceae) is used in traditional medicine in Togo. The seeds, very used, are renowned for their anti-infective and antioxidant properties. The objective of this study was to contribute to the valuation of Garcinia kola through the determination of its phytochemical groups and the evaluation of some of its biological activities. The supernatant was obtained by maceration ethanol/water (80/20) of powder from the seeds after separation. The phytochemical screening was carried out by staining and precipitation test. Antioxidant activity was determined by DPPH and FRAP test. Assessment of anti-inflammatory activity was performed by test of denaturation of bovine serum albumin protein (BSA) and denaturation of egg albumin protein (EA). The evaluation of the antibacterial activity of the extract was carried out by the agar diffusion and micro dilution method. The antibacterial effect was assessed by the MBC/MIC ratio. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, phenolic compounds, triterpenes, sterols and cardiotonic heterosides. The extract has shown an important antioxidant and anti-free radical activity concomitantly with its phytochemical makeup. The anti-inflammatory test, meanwhile, showed the ability of the extract to inhibit protein denaturation. Furthermore, antibacterial test revealed that the supernatant has activity on Staphylococcus aureus ATCC 29213 (MIC=3.125mg/mL). The result showed that the seeds of Garcinia kola have antioxidant, anti-inflammatory and antibacterial activities, which would justify phytodrug research.

Published in	International Journal of Pharmacy and Chemistry (Volume 7, Issue 6)
DOI	10.11648/j.ijpc.20210706.11
Page(s)	111-117
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Garcinia kola, Supernatant, Antioxidant, Anti-inflammatory, Antibacterial

References

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APA Style

Affo Dermane, Sandra N’samba Gbati, Batomayena Bakoma. (2021). In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). International Journal of Pharmacy and Chemistry, 7(6), 111-117. https://doi.org/10.11648/j.ijpc.20210706.11

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ACS Style

Affo Dermane; Sandra N’samba Gbati; Batomayena Bakoma. In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). Int. J. Pharm. Chem. 2021, 7(6), 111-117. doi: 10.11648/j.ijpc.20210706.11

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AMA Style

Affo Dermane, Sandra N’samba Gbati, Batomayena Bakoma. In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). Int J Pharm Chem. 2021;7(6):111-117. doi: 10.11648/j.ijpc.20210706.11

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@article{10.11648/j.ijpc.20210706.11,
  author = {Affo Dermane and Sandra N’samba Gbati and Batomayena Bakoma},
  title = {In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {7},
  number = {6},
  pages = {111-117},
  doi = {10.11648/j.ijpc.20210706.11},
  url = {https://doi.org/10.11648/j.ijpc.20210706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20210706.11},
  abstract = {The use of plants in health promotion is increasingly noteworthy in the face of the re-emergence of diseases and the limitation of conventional drugs. Garcinia kola (Clusiceae) is used in traditional medicine in Togo. The seeds, very used, are renowned for their anti-infective and antioxidant properties. The objective of this study was to contribute to the valuation of Garcinia kola through the determination of its phytochemical groups and the evaluation of some of its biological activities. The supernatant was obtained by maceration ethanol/water (80/20) of powder from the seeds after separation. The phytochemical screening was carried out by staining and precipitation test. Antioxidant activity was determined by DPPH and FRAP test. Assessment of anti-inflammatory activity was performed by test of denaturation of bovine serum albumin protein (BSA) and denaturation of egg albumin protein (EA). The evaluation of the antibacterial activity of the extract was carried out by the agar diffusion and micro dilution method. The antibacterial effect was assessed by the MBC/MIC ratio. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, phenolic compounds, triterpenes, sterols and cardiotonic heterosides. The extract has shown an important antioxidant and anti-free radical activity concomitantly with its phytochemical makeup. The anti-inflammatory test, meanwhile, showed the ability of the extract to inhibit protein denaturation. Furthermore, antibacterial test revealed that the supernatant has activity on Staphylococcus aureus ATCC 29213 (MIC=3.125mg/mL). The result showed that the seeds of Garcinia kola have antioxidant, anti-inflammatory and antibacterial activities, which would justify phytodrug research.},
 year = {2021}
}

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TY - JOUR
T1 - In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae)
AU - Affo Dermane
AU - Sandra N’samba Gbati
AU - Batomayena Bakoma
Y1 - 2021/11/25
PY - 2021
N1 - https://doi.org/10.11648/j.ijpc.20210706.11
DO - 10.11648/j.ijpc.20210706.11
T2 - International Journal of Pharmacy and Chemistry
JF - International Journal of Pharmacy and Chemistry
JO - International Journal of Pharmacy and Chemistry
SP - 111
EP - 117
PB - Science Publishing Group
SN - 2575-5749
UR - https://doi.org/10.11648/j.ijpc.20210706.11
AB - The use of plants in health promotion is increasingly noteworthy in the face of the re-emergence of diseases and the limitation of conventional drugs. Garcinia kola (Clusiceae) is used in traditional medicine in Togo. The seeds, very used, are renowned for their anti-infective and antioxidant properties. The objective of this study was to contribute to the valuation of Garcinia kola through the determination of its phytochemical groups and the evaluation of some of its biological activities. The supernatant was obtained by maceration ethanol/water (80/20) of powder from the seeds after separation. The phytochemical screening was carried out by staining and precipitation test. Antioxidant activity was determined by DPPH and FRAP test. Assessment of anti-inflammatory activity was performed by test of denaturation of bovine serum albumin protein (BSA) and denaturation of egg albumin protein (EA). The evaluation of the antibacterial activity of the extract was carried out by the agar diffusion and micro dilution method. The antibacterial effect was assessed by the MBC/MIC ratio. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, phenolic compounds, triterpenes, sterols and cardiotonic heterosides. The extract has shown an important antioxidant and anti-free radical activity concomitantly with its phytochemical makeup. The anti-inflammatory test, meanwhile, showed the ability of the extract to inhibit protein denaturation. Furthermore, antibacterial test revealed that the supernatant has activity on Staphylococcus aureus ATCC 29213 (MIC=3.125mg/mL). The result showed that the seeds of Garcinia kola have antioxidant, anti-inflammatory and antibacterial activities, which would justify phytodrug research.
VL - 7
IS - 6
ER -

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Author Information

Affo Dermane

Laboratory of Chemistry, Faculty of Health Sciences, University of Lomé, Lomé, Togo
Sandra N’samba Gbati

Laboratory of Chemistry, Faculty of Health Sciences, University of Lomé, Lomé, Togo
Batomayena Bakoma

Laboratory of Chemistry, Faculty of Health Sciences, University of Lomé, Lomé, Togo

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APA Style

Affo Dermane, Sandra N’samba Gbati, Batomayena Bakoma. (2021). In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). International Journal of Pharmacy and Chemistry, 7(6), 111-117. https://doi.org/10.11648/j.ijpc.20210706.11

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ACS Style

Affo Dermane; Sandra N’samba Gbati; Batomayena Bakoma. In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). Int. J. Pharm. Chem. 2021, 7(6), 111-117. doi: 10.11648/j.ijpc.20210706.11

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AMA Style

Affo Dermane, Sandra N’samba Gbati, Batomayena Bakoma. In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae). Int J Pharm Chem. 2021;7(6):111-117. doi: 10.11648/j.ijpc.20210706.11

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@article{10.11648/j.ijpc.20210706.11,
  author = {Affo Dermane and Sandra N’samba Gbati and Batomayena Bakoma},
  title = {In vitro Antioxidant, Anti-inflammatory and Antimicrobial Activities of Garcinia kola Seeds (Clusiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {7},
  number = {6},
  pages = {111-117},
  doi = {10.11648/j.ijpc.20210706.11},
  url = {https://doi.org/10.11648/j.ijpc.20210706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20210706.11},
  abstract = {The use of plants in health promotion is increasingly noteworthy in the face of the re-emergence of diseases and the limitation of conventional drugs. Garcinia kola (Clusiceae) is used in traditional medicine in Togo. The seeds, very used, are renowned for their anti-infective and antioxidant properties. The objective of this study was to contribute to the valuation of Garcinia kola through the determination of its phytochemical groups and the evaluation of some of its biological activities. The supernatant was obtained by maceration ethanol/water (80/20) of powder from the seeds after separation. The phytochemical screening was carried out by staining and precipitation test. Antioxidant activity was determined by DPPH and FRAP test. Assessment of anti-inflammatory activity was performed by test of denaturation of bovine serum albumin protein (BSA) and denaturation of egg albumin protein (EA). The evaluation of the antibacterial activity of the extract was carried out by the agar diffusion and micro dilution method. The antibacterial effect was assessed by the MBC/MIC ratio. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, phenolic compounds, triterpenes, sterols and cardiotonic heterosides. The extract has shown an important antioxidant and anti-free radical activity concomitantly with its phytochemical makeup. The anti-inflammatory test, meanwhile, showed the ability of the extract to inhibit protein denaturation. Furthermore, antibacterial test revealed that the supernatant has activity on Staphylococcus aureus ATCC 29213 (MIC=3.125mg/mL). The result showed that the seeds of Garcinia kola have antioxidant, anti-inflammatory and antibacterial activities, which would justify phytodrug research.},
 year = {2021}
}

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