Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi; Stephane Kevin Ndengue Langoul; Jean Michel Tekam; Pierre Rene Fotsing Kwetche

doi:doi:10.11648/j.ijpc.20220806.12

Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, Stephane Kevin Ndengue Langoul, Jean Michel Tekam, Pierre Rene Fotsing Kwetche

Published in International Journal of Pharmacy and Chemistry (Volume 8, Issue 6)

Received: 29 December 2022 Accepted: 16 January 2023 Published: 31 January 2023

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Abstract

Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.

Published in	International Journal of Pharmacy and Chemistry (Volume 8, Issue 6)
DOI	10.11648/j.ijpc.20220806.12
Page(s)	75-81
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Fresh Leaves Extract, Gardenia Aqualla, Polyphénols, Flavonoïds, Male Accessory Glands Infection

References

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Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. (2023). Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). International Journal of Pharmacy and Chemistry, 8(6), 75-81. https://doi.org/10.11648/j.ijpc.20220806.12

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ACS Style

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int. J. Pharm. Chem. 2023, 8(6), 75-81. doi: 10.11648/j.ijpc.20220806.12

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AMA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int J Pharm Chem. 2023;8(6):75-81. doi: 10.11648/j.ijpc.20220806.12

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@article{10.11648/j.ijpc.20220806.12,
  author = {Claude Berenger Ngantchouko Ngalemo and Gisele Laurel Tchiengang Tchoua and Francois Nicaise Bony and Blondin Gatien Tsawo and Patrick Yamen Mbopi and Stephane Kevin Ndengue Langoul and Jean Michel Tekam and Pierre Rene Fotsing Kwetche},
  title = {Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {8},
  number = {6},
  pages = {75-81},
  doi = {10.11648/j.ijpc.20220806.12},
  url = {https://doi.org/10.11648/j.ijpc.20220806.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220806.12},
  abstract = {Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.},
 year = {2023}
}

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TY  - JOUR
T1  - Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)
AU  - Claude Berenger Ngantchouko Ngalemo
AU  - Gisele Laurel Tchiengang Tchoua
AU  - Francois Nicaise Bony
AU  - Blondin Gatien Tsawo
AU  - Patrick Yamen Mbopi
AU  - Stephane Kevin Ndengue Langoul
AU  - Jean Michel Tekam
AU  - Pierre Rene Fotsing Kwetche
Y1  - 2023/01/31
PY  - 2023
N1  - https://doi.org/10.11648/j.ijpc.20220806.12
DO  - 10.11648/j.ijpc.20220806.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 75
EP  - 81
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20220806.12
AB  - Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.
VL  - 8
IS  - 6
ER  -

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Author Information

Claude Berenger Ngantchouko Ngalemo

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Gisele Laurel Tchiengang Tchoua

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Francois Nicaise Bony

Department of Analytical, Mineral and General Chemistry, Food Technology, Training and Research Unit, Pharmaceutical and Biological Sciences, Félix Houphouet-Boigny University, Abidjan, C?te d’Ivoire
Blondin Gatien Tsawo

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Patrick Yamen Mbopi

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Stephane Kevin Ndengue Langoul

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Jean Michel Tekam

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon
Pierre Rene Fotsing Kwetche

Higher Institute of Health Sciences, Université des Montagnes, Bangangté, Cameroon

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APA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. (2023). Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). International Journal of Pharmacy and Chemistry, 8(6), 75-81. https://doi.org/10.11648/j.ijpc.20220806.12

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ACS Style

Claude Berenger Ngantchouko Ngalemo; Gisele Laurel Tchiengang Tchoua; Francois Nicaise Bony; Blondin Gatien Tsawo; Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int. J. Pharm. Chem. 2023, 8(6), 75-81. doi: 10.11648/j.ijpc.20220806.12

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AMA Style

Claude Berenger Ngantchouko Ngalemo, Gisele Laurel Tchiengang Tchoua, Francois Nicaise Bony, Blondin Gatien Tsawo, Patrick Yamen Mbopi, et al. Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae). Int J Pharm Chem. 2023;8(6):75-81. doi: 10.11648/j.ijpc.20220806.12

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@article{10.11648/j.ijpc.20220806.12,
  author = {Claude Berenger Ngantchouko Ngalemo and Gisele Laurel Tchiengang Tchoua and Francois Nicaise Bony and Blondin Gatien Tsawo and Patrick Yamen Mbopi and Stephane Kevin Ndengue Langoul and Jean Michel Tekam and Pierre Rene Fotsing Kwetche},
  title = {Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {8},
  number = {6},
  pages = {75-81},
  doi = {10.11648/j.ijpc.20220806.12},
  url = {https://doi.org/10.11648/j.ijpc.20220806.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20220806.12},
  abstract = {Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Polyphenolic and Flavonoids Contents and Antibacterial Activity of Hydro-Ethanolic and Aqueous Extract of Fresh Leaves of Gardenia Aqualla Staph and Hutch (Rubiaceae)
AU  - Claude Berenger Ngantchouko Ngalemo
AU  - Gisele Laurel Tchiengang Tchoua
AU  - Francois Nicaise Bony
AU  - Blondin Gatien Tsawo
AU  - Patrick Yamen Mbopi
AU  - Stephane Kevin Ndengue Langoul
AU  - Jean Michel Tekam
AU  - Pierre Rene Fotsing Kwetche
Y1  - 2023/01/31
PY  - 2023
N1  - https://doi.org/10.11648/j.ijpc.20220806.12
DO  - 10.11648/j.ijpc.20220806.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 75
EP  - 81
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20220806.12
AB  - Erectile dysfunction has become for two decades, a real public health problem. Its prevalence is estimated at 322 million in 2025. Its management is based on the administration of phosphodiesterase inhibitors alone or in combination. Infections have been rarely mentioned although E coli, Staphylococcus aureus, Klebsiella pneumoniae or Serratia marcescens are recognized as factors of erectile dysfunction. The purpose of this study was to evaluate the phytochemical profile of the aqueous (AE) and hydroethanolic (HE) extracts of the fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. The aim of this study was to evaluate phytochemical profile of aqueous (AE) and hydroethanolic (HE) extracts of fresh leaves of Gardenia aqualla and to determine their antibacterial potential on bacteria involved in male accessory gland infections. Phytochemical profile highlighted the presence of alkaloids, polyphenols with concentrations of 434.5 ± 14.5 mgEq Tannic acid/g of dried extract greater than 249.5±18.3 mgEq Tannic acid/g of dried extract found in AE including flavonoids whose concentrations is 184.9 ± 6.3 μgEqRutin/g of dry extract in HE against 183.0±9.1 μgEqRutin/g of dried extract in AE. Antibacterial tests showed equivalence of activity on E coli and S aureus strains with MICs of 100 mg/mL and 50mg/mL respectively and only HE showed activity on Klebsiella pneumoniae and Serratia marcescens which had the lowest MIC 6.25 mg/mL.
VL  - 8
IS  - 6
ER  -

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