Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with <i>Saccharomyces Cerevisiae </i>and<i> Aspergillus Niger</i>

Debebe Landina Lata; Lukas Birhanu; Mohammed Lengichow; Getnet Degemu; Tsegaye Atnaf; Ayansa Kebenessa

doi:doi:10.11648/j.ijpc.20241003.12

Research Article |

| Peer-Reviewed

Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger

Debebe Landina Lata^*

, Lukas Birhanu, Mohammed Lengichow

, Getnet Degemu, Tsegaye Atnaf, Ayansa Kebenessa

Published in International Journal of Pharmacy and Chemistry (Volume 10, Issue 3)

Received: 4 December 2024 Accepted: 17 December 2024 Published: 30 December 2024

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Bioethanol, a sustainable alternative fuel derived from organic materials, is essential for addressing global energy demands and environmental concerns. This study aimed to produce bioethanol from banana and mango peels using co-cultures of Aspergillus niger and Saccharomyces cerevisiae through a simultaneous saccharification and fermentation (SSF) process. Fully ripened banana and mango peels, obtained from a local market, were dried, ground into fine particles, and used as substrates for bioethanol production. The fermentation process was carried out by sequentially inoculating the substrates with Aspergillus niger to enhance starch hydrolysis, followed by Saccharomyces cerevisiae to facilitate fermentation. The process lasted for 7 days under controlled conditions, with a pH range of 5.5-6.0 and a temperature of 28±2°C. Among the tested samples, the mixed substrate of banana and mango peels yielded the highest ethanol concentration at 79% (w/v), while mango peels alone produced 74% and banana peels produced 71%. The enhanced performance of the mixed substrate highlights the synergistic effect of combining different fruit wastes. The presence of Aspergillus niger played a crucial role in breaking down complex starches into simpler sugars, enabling Saccharomyces cerevisiae to effectively convert these sugars into ethanol. This study demonstrates the potential of fruit waste, specifically banana and mango peels, as cost-effective and sustainable raw materials for bioethanol production, providing a promising alternative to fossil fuels. Future research should focus on optimizing fermentation conditions, exploring the potential of additional fruit waste substrates, and scaling up the process for commercial viability.

Published in	International Journal of Pharmacy and Chemistry (Volume 10, Issue 3)
DOI	10.11648/j.ijpc.20241003.12
Page(s)	39-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Agricultural Waste, Aspergillus niger, Bioethanol, Fermentation, Saccharomyces cerevisiae

References

[1]	Afrida Sitompul, Selvia Sarungu, Karnila Willard, Hamriani Ryka, Simon Tampang, Junaesar Tangke, Bodhi Dharma. (2021). Production of bioethanol from dragon fruit wastes by using Aspergillus niger and Saccharomyces cerevisiae. Advances in Biological Sciences Research, 16, 1–4. https://doi.org/10.2991/absr.2017.16.1
[2]	Stanley, H., Ofuru, R., Onwukwe, C. D. (2017). Production of bioethanol from plantain peels using Aspergillus spp. and Saccharomyces spp. Scientia Africana, 16(1), 1–8. https://doi.org/10.4314/sa.v16i1.1
[3]	Itelima, J. U., Onwuliri, F. I., Onwuliri, E. A., Onyimba, I. A., Oforji, S. O. (2021). Lignocellulosic biomass as a renewable resource for bioethanol production. International Journal of Environmental Science and Technology, 18(6), 1981–1992. https://doi.org/10.1007/s13762-021-03216-7
[4]	Aminu, B., Bukar, A., Ado, A., Bello, I. (2022). Isolation of Saccharomyces cerevisiae and Aspergillus niger strains from environmental samples for bioethanol production. Journal of Microbiology and Biotechnology, 11(3), 12–20. https://doi.org/10.9734/jmb/2022/v11i330118
[5]	Kadar, S. O., Szengyel, Z., Reckey, M. (2021). Lignocellulosic biomass resources and their potential for bioethanol production: A comprehensive review. Bioresource Technology, 325, 124752. https://doi.org/10.1016/j.biortech.2021.124752
[6]	Gusakov, A. P., Sinitsyn, J. A., Manenkova, O. V., Prostas, O. V. (2022). Advances in the biotechnological conversion of lignocellulosic biomass to bioethanol: Challenges and prospects. Renewable and Sustainable Energy Reviews, 137, 110571. https://doi.org/10.1016/j.rser.2020.110571
[7]	Mizik, T. (2021). Economic aspects and sustainability of ethanol production: A systematic literature review. Energies, 14, 6137. https://doi.org/10.3390/en14196137
[8]	Rath, S., Kumar, A., Singh, H., Kumar, Y., Kiran, J., Pankaj, S. K. (2022). Bioethanol production from waste potatoes as an environmental waste management and sustainable energy by using co-cultures Aspergillus niger and Saccharomyces cerevisiae. International Journal of Advanced Research, 2(4), 1-11.
[9]	Oadipo, O., Ilesanmi, T. M., Odeleye, A. (2020). Microbial diversity in soil ecosystems: Isolation of Aspergillus strains and their biotechnological applications. African Journal of Microbiology, 9(2), 101–109. https://doi.org/10.1023/ajm.2020.00920
[10]	Derman, E., Abdulla, R., Marbawi, H., Sabullah, M. K., Gansau, J. A., Ravindra, P. (2021). Isolation of fungal strains from soil for bioethanol production: Characterization and applications. Fermentation Technology, 5(2), 1–9. https://doi.org/10.3390/fermentation5020045
[11]	Villadiego-del V, A. E., Sarmiento Zea, L., Leon-Pulido, J., Rojas-Perez, M., Lilia. (2021). Bioethanol production from yam (Dioscorea rotundata) using simultaneous saccharification and fermentation. Techno Logas, 24(5), 1-10.
[12]	Derman, E., Abdulla, R., Marbawi, H., Sabullah, M. K., Gansau, J. A., Ravindra, P. (2022). Simultaneous saccharification and fermentation of empty fruit bunches of palms for bioethanol production using a microbial consortium of S. cerevisiae and T. harzianum. Fermentation, 8(295), 1–12. https://doi.org/10.3390/fermentation8040295
[13]	Brooks, A. (2020). Ethanol production potential of local yeast strains isolated from ripe banana peels. African Journal of Biotechnology, 7, 1–11.
[14]	Itelima, J., Onwuliri, F., Onwuliri, E., Onyimba, I., Oforji, S. (2022). Bioethanol production from banana, plantain, and pineapple peels by simultaneous saccharification and fermentation process. International Journal of Environmental Science and Development, 4(2), 1-4.
[15]	Kitson-Hytey, M., MacDonald, B., Fei-Baffoe, B., Sackey, L., Miezah, K. (2023). Production of bioethanol from plantain and yam peels using Aspergillus niger and Saccharomyces cerevisiae. Biomass Conversion and Biorefinery, 14, 1-9.
[16]	Singh, A., Dubey, P., Bhatt, K., Sharma, D. (2021). Advances in biomass-based bioethanol production: Sustainable alternatives for biofuels. Renewable and Sustainable Energy Reviews, 129, 109899. https://doi.org/10.1016/j.rser.2020.109899
[17]	Ameen, F., Baloch, Z., Ashraf, M., Qureshi, R. (2020). Bioethanol production from waste biomass: A review of sustainable strategies and applications. Journal of Cleaner Production, 243, 118554. https://doi.org/10.1016/j.jclepro.2019.118554
[18]	Gholamian, S., Jahanbakhshi, A., Shaterian, A., Sharifi, A., Ghanbari, A. (2021). Bioethanol production from agricultural waste: Recent advances and future prospects. Bioresource Technology, 339, 125485. https://doi.org/10.1016/j.biortech.2021.125485
[19]	Aminu, B., Bukar, A., Ado, A., Bello, I. (2021). Bioethanol production from agricultural waste materials using co-cultures of Aspergillus niger and Saccharomyces cerevisiae: Evaluation of starch degradation. International Journal of Biotechnology, 8(3), 22–29. https://doi.org/10.9734/ijb/2021/v8i330113
[20]	Ahamad, Y. T., Thouseef, M., Panduranga, M. G. (2022). Comparative analysis of Aspergillus niger strains for starch degradation and bioethanol production. International Journal of Science and Technology, 9(2), 1–12. https://doi.org/10.1234/ijst.2022.090202
[21]	Oadipo, O., Ilesanmi, T. M., Odeleye, A. (2022). Manufacture of bio-ethanol from waste material (cassava peels) utilizing some microbes. Asian Journal of Research in Microbiology, 12(4), 1–5. https://doi.org/10.9734/ajrim/2022/v12i430305

Cite This Article

Plain Text BibTeX RIS

APA Style

Lata, D. L., Birhanu, L., Lengichow, M., Degemu, G., Atnaf, T., et al. (2024). Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. International Journal of Pharmacy and Chemistry, 10(3), 39-45. https://doi.org/10.11648/j.ijpc.20241003.12

Copy | Download

ACS Style

Lata, D. L.; Birhanu, L.; Lengichow, M.; Degemu, G.; Atnaf, T., et al. Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. Int. J. Pharm. Chem. 2024, 10(3), 39-45. doi: 10.11648/j.ijpc.20241003.12

Copy | Download

AMA Style

Lata DL, Birhanu L, Lengichow M, Degemu G, Atnaf T, et al. Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. Int J Pharm Chem. 2024;10(3):39-45. doi: 10.11648/j.ijpc.20241003.12

Copy | Download

@article{10.11648/j.ijpc.20241003.12,
  author = {Debebe Landina Lata and Lukas Birhanu and Mohammed Lengichow and Getnet Degemu and Tsegaye Atnaf and Ayansa Kebenessa},
  title = {Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger
},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {10},
  number = {3},
  pages = {39-45},
  doi = {10.11648/j.ijpc.20241003.12},
  url = {https://doi.org/10.11648/j.ijpc.20241003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20241003.12},
  abstract = {Bioethanol, a sustainable alternative fuel derived from organic materials, is essential for addressing global energy demands and environmental concerns. This study aimed to produce bioethanol from banana and mango peels using co-cultures of Aspergillus niger and Saccharomyces cerevisiae through a simultaneous saccharification and fermentation (SSF) process. Fully ripened banana and mango peels, obtained from a local market, were dried, ground into fine particles, and used as substrates for bioethanol production. The fermentation process was carried out by sequentially inoculating the substrates with Aspergillus niger to enhance starch hydrolysis, followed by Saccharomyces cerevisiae to facilitate fermentation. The process lasted for 7 days under controlled conditions, with a pH range of 5.5-6.0 and a temperature of 28±2°C. Among the tested samples, the mixed substrate of banana and mango peels yielded the highest ethanol concentration at 79% (w/v), while mango peels alone produced 74% and banana peels produced 71%. The enhanced performance of the mixed substrate highlights the synergistic effect of combining different fruit wastes. The presence of Aspergillus niger played a crucial role in breaking down complex starches into simpler sugars, enabling Saccharomyces cerevisiae to effectively convert these sugars into ethanol. This study demonstrates the potential of fruit waste, specifically banana and mango peels, as cost-effective and sustainable raw materials for bioethanol production, providing a promising alternative to fossil fuels. Future research should focus on optimizing fermentation conditions, exploring the potential of additional fruit waste substrates, and scaling up the process for commercial viability.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger

AU  - Debebe Landina Lata
AU  - Lukas Birhanu
AU  - Mohammed Lengichow
AU  - Getnet Degemu
AU  - Tsegaye Atnaf
AU  - Ayansa Kebenessa
Y1  - 2024/12/30
PY  - 2024
N1  - https://doi.org/10.11648/j.ijpc.20241003.12
DO  - 10.11648/j.ijpc.20241003.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 39
EP  - 45
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20241003.12
AB  - Bioethanol, a sustainable alternative fuel derived from organic materials, is essential for addressing global energy demands and environmental concerns. This study aimed to produce bioethanol from banana and mango peels using co-cultures of Aspergillus niger and Saccharomyces cerevisiae through a simultaneous saccharification and fermentation (SSF) process. Fully ripened banana and mango peels, obtained from a local market, were dried, ground into fine particles, and used as substrates for bioethanol production. The fermentation process was carried out by sequentially inoculating the substrates with Aspergillus niger to enhance starch hydrolysis, followed by Saccharomyces cerevisiae to facilitate fermentation. The process lasted for 7 days under controlled conditions, with a pH range of 5.5-6.0 and a temperature of 28±2°C. Among the tested samples, the mixed substrate of banana and mango peels yielded the highest ethanol concentration at 79% (w/v), while mango peels alone produced 74% and banana peels produced 71%. The enhanced performance of the mixed substrate highlights the synergistic effect of combining different fruit wastes. The presence of Aspergillus niger played a crucial role in breaking down complex starches into simpler sugars, enabling Saccharomyces cerevisiae to effectively convert these sugars into ethanol. This study demonstrates the potential of fruit waste, specifically banana and mango peels, as cost-effective and sustainable raw materials for bioethanol production, providing a promising alternative to fossil fuels. Future research should focus on optimizing fermentation conditions, exploring the potential of additional fruit waste substrates, and scaling up the process for commercial viability.

VL  - 10
IS  - 3
ER  -

Copy | Download

Author Information

Debebe Landina Lata

Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

Contact Email

http://orcid.org/0009-0005-8402-4839
Lukas Birhanu

Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

Contact Email
Mohammed Lengichow

Department of Biology, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

Contact Email

http://orcid.org/0000-0002-0127-5204
Getnet Degemu

Department of Computer Science, College of Computing and Informatics, Wolkite University, Wolkite, Ethiopia

Contact Email
Tsegaye Atnaf

Department of Chemistry, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

Contact Email
Ayansa Kebenessa

Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia

Contact Email

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Lata, D. L., Birhanu, L., Lengichow, M., Degemu, G., Atnaf, T., et al. (2024). Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. International Journal of Pharmacy and Chemistry, 10(3), 39-45. https://doi.org/10.11648/j.ijpc.20241003.12

Copy | Download

ACS Style

Lata, D. L.; Birhanu, L.; Lengichow, M.; Degemu, G.; Atnaf, T., et al. Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. Int. J. Pharm. Chem. 2024, 10(3), 39-45. doi: 10.11648/j.ijpc.20241003.12

Copy | Download

AMA Style

Lata DL, Birhanu L, Lengichow M, Degemu G, Atnaf T, et al. Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger. Int J Pharm Chem. 2024;10(3):39-45. doi: 10.11648/j.ijpc.20241003.12

Copy | Download

@article{10.11648/j.ijpc.20241003.12,
  author = {Debebe Landina Lata and Lukas Birhanu and Mohammed Lengichow and Getnet Degemu and Tsegaye Atnaf and Ayansa Kebenessa},
  title = {Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger
},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {10},
  number = {3},
  pages = {39-45},
  doi = {10.11648/j.ijpc.20241003.12},
  url = {https://doi.org/10.11648/j.ijpc.20241003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20241003.12},
  abstract = {Bioethanol, a sustainable alternative fuel derived from organic materials, is essential for addressing global energy demands and environmental concerns. This study aimed to produce bioethanol from banana and mango peels using co-cultures of Aspergillus niger and Saccharomyces cerevisiae through a simultaneous saccharification and fermentation (SSF) process. Fully ripened banana and mango peels, obtained from a local market, were dried, ground into fine particles, and used as substrates for bioethanol production. The fermentation process was carried out by sequentially inoculating the substrates with Aspergillus niger to enhance starch hydrolysis, followed by Saccharomyces cerevisiae to facilitate fermentation. The process lasted for 7 days under controlled conditions, with a pH range of 5.5-6.0 and a temperature of 28±2°C. Among the tested samples, the mixed substrate of banana and mango peels yielded the highest ethanol concentration at 79% (w/v), while mango peels alone produced 74% and banana peels produced 71%. The enhanced performance of the mixed substrate highlights the synergistic effect of combining different fruit wastes. The presence of Aspergillus niger played a crucial role in breaking down complex starches into simpler sugars, enabling Saccharomyces cerevisiae to effectively convert these sugars into ethanol. This study demonstrates the potential of fruit waste, specifically banana and mango peels, as cost-effective and sustainable raw materials for bioethanol production, providing a promising alternative to fossil fuels. Future research should focus on optimizing fermentation conditions, exploring the potential of additional fruit waste substrates, and scaling up the process for commercial viability.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Exploring Bio-Ethanol Production from Fruit Wastes Through Fermentation with Saccharomyces Cerevisiae and Aspergillus Niger

AU  - Debebe Landina Lata
AU  - Lukas Birhanu
AU  - Mohammed Lengichow
AU  - Getnet Degemu
AU  - Tsegaye Atnaf
AU  - Ayansa Kebenessa
Y1  - 2024/12/30
PY  - 2024
N1  - https://doi.org/10.11648/j.ijpc.20241003.12
DO  - 10.11648/j.ijpc.20241003.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 39
EP  - 45
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20241003.12
AB  - Bioethanol, a sustainable alternative fuel derived from organic materials, is essential for addressing global energy demands and environmental concerns. This study aimed to produce bioethanol from banana and mango peels using co-cultures of Aspergillus niger and Saccharomyces cerevisiae through a simultaneous saccharification and fermentation (SSF) process. Fully ripened banana and mango peels, obtained from a local market, were dried, ground into fine particles, and used as substrates for bioethanol production. The fermentation process was carried out by sequentially inoculating the substrates with Aspergillus niger to enhance starch hydrolysis, followed by Saccharomyces cerevisiae to facilitate fermentation. The process lasted for 7 days under controlled conditions, with a pH range of 5.5-6.0 and a temperature of 28±2°C. Among the tested samples, the mixed substrate of banana and mango peels yielded the highest ethanol concentration at 79% (w/v), while mango peels alone produced 74% and banana peels produced 71%. The enhanced performance of the mixed substrate highlights the synergistic effect of combining different fruit wastes. The presence of Aspergillus niger played a crucial role in breaking down complex starches into simpler sugars, enabling Saccharomyces cerevisiae to effectively convert these sugars into ethanol. This study demonstrates the potential of fruit waste, specifically banana and mango peels, as cost-effective and sustainable raw materials for bioethanol production, providing a promising alternative to fossil fuels. Future research should focus on optimizing fermentation conditions, exploring the potential of additional fruit waste substrates, and scaling up the process for commercial viability.

VL  - 10
IS  - 3
ER  -

Copy | Download