This study focuses on developing a highly accurate and reproducible High-Performance Liquid Chromatography technique of β-sitosterol for the quantification. Widely, a compound used as a treatment of burns and skin ulcers. This developed method ensures reliable separation, identification, and quantification of β-sitosterol in pharmaceutical formulations. Accuracy was assessed through recovery studies at three levels (80%, 100%, and 120%). The acceptance criterion was a recovery rate between 98% and 102%, which was successfully met. The detection limit (LOD) is defined as the lowest concentration of an analyte that can be detected, though not necessarily quantified. Typically, a signal-to-noise (S/N) ratio of 3: 1 is considered acceptable for estimating the LOD. The quantification limit (LOQ) refers to the lowest concentration that can be quantitatively measured with acceptable precision and accuracy, usually determined at a signal-to-noise ratio of 10: 1. The LOD is calculated using the formula 3.3 × (Sy/x / b), while the LOQ is calculated as 10 × (Sy/x / b). In this method, the LOD was found to be 2 µg/mL, and the LOQ was 6 µg/mL. The LOD and LOQ were determined based on the standard deviation of the response (σ) and the slope (S) of the calibration curve. The calculated LOD was 1.52 µg/mL.
| Published in | Research and Innovation (Volume 1, Issue 1) |
| DOI | 10.11648/j.ri.20250101.20 |
| Page(s) | 77-82 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Analytical, HPLC, β-Sitosterol, Treatment
| [1] | Snyder, L. R., Kirkland, J. J., & Dolan, J. W. (2010). Introduction to Modern Liquid Chromatography (3rd ed.). Wiley. |
| [2] | Moghadasian, M. H. (2000). Pharmacological properties of plant sterols. Lipids in Health and Disease, 39(12), 1139-1145. |
| [3] | ICH Q2(R1). (2005). Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization. |
| [4] | Gupta, A., & Mishra, A. (2012). Healing potential of β-sitosterol with antioxidant activity in experimental burns. Burns, 38(6), 793-797. |
| [5] | Dong, M. W. (2006). Modern HPLC for Practicing Scientists. Wiley-Interscience. |
| [6] | Mahboob, N., Mustafa, T and Nail, A., (2022). Development of sodium hydroxide-induced homogenous liquid-liquid extraction effervescent assisted dispersive liquid-liquid microextraction based on deep eutectic solvents; application in the extraction of phytosterols from cow cream samples. Journal of Food Composition and Analysis, 106: 104291. |
| [7] | Novotny L, Abdel-Hamid ME, Hunakova L, Saeidnia S, Manayi A, Gohari AR, (2017). Anticancer potential of β-sitosterol. International Journal of Clinical Pharmacology & Pharmacotherapy. 2017; 2(10.15344): 2456-3501. |
| [8] | Li Y, Meng Q, Yang M, Liu D, Hou X and Tang L,. (2019). Current trends in drug metabolism and pharmacokinetics. Acta Pharmaceutica Sinica 2019; 9(6): 1113-1144. |
| [9] | K. Khonsa, D. L. Setyaningrum, A. H. Saputro, T. Amelia, S. Ibrahim and S. Damayanti. (2022): Analysis Of Β-Sitosterol in Supplement Using High-Performance Liquid Chromatography: Development and Validation. Rasayan J. Chem., 15(3). |
| [10] |
Al-Arnoot, S., Abdullah, Q. Y., Alkhyat, S. H., Almahbashi, A. A., & Al-Nowihi, M. Human and animal brucellosis in Yemen. Journal of Human Virology & Retrovirology. 2017, 5(4), 00162.
https://doi. org/10.15406/jhvrv.2017.05.00162 |
| [11] | Nowihi, M. A., Abdullah, Q. Y. M., Alkhyat, S. H., Almahbashi, A. A., Al-Thobahni, A., & Al-Bana, M. N. Comparison of standard agglutination test and enzyme-linked immunosorbent assay to detect Brucella infection in Yemeni pregnant women. Journal of Microbiology & Experiment. 2017, 5(5), 11-12. |
| [12] | Abdullah, Q. Y. M., Alkhyat, S. H., & Almahbashi, A. A. Seroprevalence of Brucella infection among pregnant women in Sana’a City, Yemen. Biometrics & Biostatistics International Journal. 2018, 7(5), 445-451. |
| [13] | Al-Arnoot, S., Alghalibi, S. M., Abdullah, Q. Y. M., & Al-Thobhani, A. Screening for susceptibility to cytomegalovirus infection among pregnant women in Yemen. Journal of Gynecology & Women’s Health. 2020, 18(3), 4. |
APA Style
Qasem, M. H. A., Al-Nowihi, M., AL-ansi, Y., Alrobassi, M. N., Kadari, A. A., et al. (2025). Development of an HPLC Analytical Method for β-Sitosterol Used as a Treatment of Burns and Skin Ulcers. Research and Innovation, 1(1), 77-82. https://doi.org/10.11648/j.ri.20250101.20
ACS Style
Qasem, M. H. A.; Al-Nowihi, M.; AL-ansi, Y.; Alrobassi, M. N.; Kadari, A. A., et al. Development of an HPLC Analytical Method for β-Sitosterol Used as a Treatment of Burns and Skin Ulcers. Res. Innovation 2025, 1(1), 77-82. doi: 10.11648/j.ri.20250101.20
AMA Style
Qasem MHA, Al-Nowihi M, AL-ansi Y, Alrobassi MN, Kadari AA, et al. Development of an HPLC Analytical Method for β-Sitosterol Used as a Treatment of Burns and Skin Ulcers. Res Innovation. 2025;1(1):77-82. doi: 10.11648/j.ri.20250101.20
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ri.20250101.20,
author = {Mouath Heal Abdullaha Qasem and Mofeed Al-Nowihi and Yasser AL-ansi and Mohammed Najeeb Alrobassi and Ahmed Ahmed Kadari and Tolbi Al-sandarous and Assem Al-Thobhani},
title = {Development of an HPLC Analytical Method for β-Sitosterol Used as a Treatment of Burns and Skin Ulcers},
journal = {Research and Innovation},
volume = {1},
number = {1},
pages = {77-82},
doi = {10.11648/j.ri.20250101.20},
url = {https://doi.org/10.11648/j.ri.20250101.20},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ri.20250101.20},
abstract = {This study focuses on developing a highly accurate and reproducible High-Performance Liquid Chromatography technique of β-sitosterol for the quantification. Widely, a compound used as a treatment of burns and skin ulcers. This developed method ensures reliable separation, identification, and quantification of β-sitosterol in pharmaceutical formulations. Accuracy was assessed through recovery studies at three levels (80%, 100%, and 120%). The acceptance criterion was a recovery rate between 98% and 102%, which was successfully met. The detection limit (LOD) is defined as the lowest concentration of an analyte that can be detected, though not necessarily quantified. Typically, a signal-to-noise (S/N) ratio of 3: 1 is considered acceptable for estimating the LOD. The quantification limit (LOQ) refers to the lowest concentration that can be quantitatively measured with acceptable precision and accuracy, usually determined at a signal-to-noise ratio of 10: 1. The LOD is calculated using the formula 3.3 × (Sy/x / b), while the LOQ is calculated as 10 × (Sy/x / b). In this method, the LOD was found to be 2 µg/mL, and the LOQ was 6 µg/mL. The LOD and LOQ were determined based on the standard deviation of the response (σ) and the slope (S) of the calibration curve. The calculated LOD was 1.52 µg/mL.},
year = {2025}
}
TY - JOUR T1 - Development of an HPLC Analytical Method for β-Sitosterol Used as a Treatment of Burns and Skin Ulcers AU - Mouath Heal Abdullaha Qasem AU - Mofeed Al-Nowihi AU - Yasser AL-ansi AU - Mohammed Najeeb Alrobassi AU - Ahmed Ahmed Kadari AU - Tolbi Al-sandarous AU - Assem Al-Thobhani Y1 - 2025/12/19 PY - 2025 N1 - https://doi.org/10.11648/j.ri.20250101.20 DO - 10.11648/j.ri.20250101.20 T2 - Research and Innovation JF - Research and Innovation JO - Research and Innovation SP - 77 EP - 82 PB - Science Publishing Group UR - https://doi.org/10.11648/j.ri.20250101.20 AB - This study focuses on developing a highly accurate and reproducible High-Performance Liquid Chromatography technique of β-sitosterol for the quantification. Widely, a compound used as a treatment of burns and skin ulcers. This developed method ensures reliable separation, identification, and quantification of β-sitosterol in pharmaceutical formulations. Accuracy was assessed through recovery studies at three levels (80%, 100%, and 120%). The acceptance criterion was a recovery rate between 98% and 102%, which was successfully met. The detection limit (LOD) is defined as the lowest concentration of an analyte that can be detected, though not necessarily quantified. Typically, a signal-to-noise (S/N) ratio of 3: 1 is considered acceptable for estimating the LOD. The quantification limit (LOQ) refers to the lowest concentration that can be quantitatively measured with acceptable precision and accuracy, usually determined at a signal-to-noise ratio of 10: 1. The LOD is calculated using the formula 3.3 × (Sy/x / b), while the LOQ is calculated as 10 × (Sy/x / b). In this method, the LOD was found to be 2 µg/mL, and the LOQ was 6 µg/mL. The LOD and LOQ were determined based on the standard deviation of the response (σ) and the slope (S) of the calibration curve. The calculated LOD was 1.52 µg/mL. VL - 1 IS - 1 ER -
Faculty of Science, Sana’a University, Sana'a, Yemen
Information