Prevalence of <i>Escherichia coli</i> and <i>Salmonella spp</i> Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production

Stéphanie Lynseh Carine Sita Bénao; Amana Métuor Dabiré; Rahimatou Yasmine Wendkuni Tiemtoré; Pegdwende Abel Sorgho; Guigma Géraud Joël; Jacques Simporé

doi:doi:10.11648/j.bmb.20230801.12

Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production

Stéphanie Lynseh Carine Sita Bénao, Amana Métuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Pegdwende Abel Sorgho, Guigma Géraud Joël, Jacques Simporé

Published in Biochemistry and Molecular Biology (Volume 8, Issue 1)

Received: 30 January 2023 Accepted: 28 February 2023 Published: 13 April 2023

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Abstract

The Increase in antibiotic resistance is a threat to the world health. Some resistant bacteria have the ability to transfer from animals to humans either through their stool or through their flesh. The spread and emergence of antibiotic resistance is therefore a public health problem. For this study, a total of 101 chickens were randomly selected from the busiest recreation areas of Ouagadougou and fresh chicken droppings taken directly from the animal's intestines were collected. Of the samples analyzed, 78.21% and 9.90% respectively contained Escherichia coli and Salmonella spp. For the 10 isolates of Salmonella spp, a resistance to CTX-M of 10%, 10% to CAZ, 10% to CRO and finally 70% resistant to AMC was observed. For the 79 strains of Escherichia coli, it was observed an absence of resistance to CTX-M, 3.79% to CAS, 3.79% to CRO and 84.81% of strain resistant to AMC. Four (4) mutil-resistant strains were identified and resistance genes were observed in 2 of these strains. The study revealed the presence of extended spectrum beta-lactamase genes in Enterobacteriaceae contained in chicken feces. A high consumption of flambé chickens infected or contaminated by these germs is then likely to increase the risk of development and spread of bacteria resistant to antibiotics in humans.

Published in	Biochemistry and Molecular Biology (Volume 8, Issue 1)
DOI	10.11648/j.bmb.20230801.12
Page(s)	5-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Extended Spectrum Beta-Lactamases (ESBL), CTX-M Gene, PCR

References

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[5]	A. Hadjer, “Isolement et identification des salmonelles à partir de poulets de chair et des eaux usées,” Université Hassiba BEN BOUALI, 2016.
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[8]	M. I. Mirabaud, “Entérobactéries à Beta-lactamases à spectre élargi en pédiatrie en 1996,” Université de Geneve, 2003.
[9]	C. Zhao et al., “Serovars in Retail Chicken, Turkey, Pork, and Beef from the Greater Washington, D. C., Area,” Appl. Environnemental Microbiol., vol. 67, no. 12, pp. 5431–5436, 2001, doi: 10.1128/AEM.67.12.5431.
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[12]	F. Vasaï, “Etude de la composition du microbiote intestinal des canards. Impact du gavage, de l’ajout d’un probiotique (Lactobacillus sakei) et d’un composé organométallique (cadmium),” Université de Pau, 2013.
[13]	A. A. Moawad et al., “Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers,” Gut Pathog., vol. 9, no. 57, pp. 1–13, 2017, doi: 10.1186/s13099-017-0206-9.
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Stéphanie Lynseh Carine Sita Bénao, Amana Métuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Pegdwende Abel Sorgho, Guigma Géraud Joël, et al. (2023). Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochemistry and Molecular Biology, 8(1), 5-11. https://doi.org/10.11648/j.bmb.20230801.12

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ACS Style

Stéphanie Lynseh Carine Sita Bénao; Amana Métuor Dabiré; Rahimatou Yasmine Wendkuni Tiemtoré; Pegdwende Abel Sorgho; Guigma Géraud Joël, et al. Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochem. Mol. Biol. 2023, 8(1), 5-11. doi: 10.11648/j.bmb.20230801.12

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AMA Style

Stéphanie Lynseh Carine Sita Bénao, Amana Métuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Pegdwende Abel Sorgho, Guigma Géraud Joël, et al. Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochem Mol Biol. 2023;8(1):5-11. doi: 10.11648/j.bmb.20230801.12

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@article{10.11648/j.bmb.20230801.12,
  author = {Stéphanie Lynseh Carine Sita Bénao and Amana Métuor Dabiré and Rahimatou Yasmine Wendkuni Tiemtoré and Pegdwende Abel Sorgho and Guigma Géraud Joël and Jacques Simporé},
  title = {Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production},
  journal = {Biochemistry and Molecular Biology},
  volume = {8},
  number = {1},
  pages = {5-11},
  doi = {10.11648/j.bmb.20230801.12},
  url = {https://doi.org/10.11648/j.bmb.20230801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230801.12},
  abstract = {The Increase in antibiotic resistance is a threat to the world health. Some resistant bacteria have the ability to transfer from animals to humans either through their stool or through their flesh. The spread and emergence of antibiotic resistance is therefore a public health problem. For this study, a total of 101 chickens were randomly selected from the busiest recreation areas of Ouagadougou and fresh chicken droppings taken directly from the animal's intestines were collected. Of the samples analyzed, 78.21% and 9.90% respectively contained Escherichia coli and Salmonella spp. For the 10 isolates of Salmonella spp, a resistance to CTX-M of 10%, 10% to CAZ, 10% to CRO and finally 70% resistant to AMC was observed. For the 79 strains of Escherichia coli, it was observed an absence of resistance to CTX-M, 3.79% to CAS, 3.79% to CRO and 84.81% of strain resistant to AMC. Four (4) mutil-resistant strains were identified and resistance genes were observed in 2 of these strains. The study revealed the presence of extended spectrum beta-lactamase genes in Enterobacteriaceae contained in chicken feces. A high consumption of flambé chickens infected or contaminated by these germs is then likely to increase the risk of development and spread of bacteria resistant to antibiotics in humans.},
 year = {2023}
}

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TY  - JOUR
T1  - Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production
AU  - Stéphanie Lynseh Carine Sita Bénao
AU  - Amana Métuor Dabiré
AU  - Rahimatou Yasmine Wendkuni Tiemtoré
AU  - Pegdwende Abel Sorgho
AU  - Guigma Géraud Joël
AU  - Jacques Simporé
Y1  - 2023/04/13
PY  - 2023
N1  - https://doi.org/10.11648/j.bmb.20230801.12
DO  - 10.11648/j.bmb.20230801.12
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 5
EP  - 11
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20230801.12
AB  - The Increase in antibiotic resistance is a threat to the world health. Some resistant bacteria have the ability to transfer from animals to humans either through their stool or through their flesh. The spread and emergence of antibiotic resistance is therefore a public health problem. For this study, a total of 101 chickens were randomly selected from the busiest recreation areas of Ouagadougou and fresh chicken droppings taken directly from the animal's intestines were collected. Of the samples analyzed, 78.21% and 9.90% respectively contained Escherichia coli and Salmonella spp. For the 10 isolates of Salmonella spp, a resistance to CTX-M of 10%, 10% to CAZ, 10% to CRO and finally 70% resistant to AMC was observed. For the 79 strains of Escherichia coli, it was observed an absence of resistance to CTX-M, 3.79% to CAS, 3.79% to CRO and 84.81% of strain resistant to AMC. Four (4) mutil-resistant strains were identified and resistance genes were observed in 2 of these strains. The study revealed the presence of extended spectrum beta-lactamase genes in Enterobacteriaceae contained in chicken feces. A high consumption of flambé chickens infected or contaminated by these germs is then likely to increase the risk of development and spread of bacteria resistant to antibiotics in humans.
VL  - 8
IS  - 1
ER  -

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Author Information

Stéphanie Lynseh Carine Sita Bénao

Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University Joseph Ki Zerbo, Ouagadougou, Burkina Faso
Amana Métuor Dabiré

Department of Biochemistry-Microbiology, University of Dédougou, Dédougou, Burkina Faso
Rahimatou Yasmine Wendkuni Tiemtoré

Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University Joseph Ki Zerbo, Ouagadougou, Burkina Faso
Pegdwende Abel Sorgho

Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University Joseph Ki Zerbo, Ouagadougou, Burkina Faso
Guigma Géraud Joël
Jacques Simporé

Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University Joseph Ki Zerbo, Ouagadougou, Burkina Faso

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APA Style

Stéphanie Lynseh Carine Sita Bénao, Amana Métuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Pegdwende Abel Sorgho, Guigma Géraud Joël, et al. (2023). Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochemistry and Molecular Biology, 8(1), 5-11. https://doi.org/10.11648/j.bmb.20230801.12

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ACS Style

Stéphanie Lynseh Carine Sita Bénao; Amana Métuor Dabiré; Rahimatou Yasmine Wendkuni Tiemtoré; Pegdwende Abel Sorgho; Guigma Géraud Joël, et al. Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochem. Mol. Biol. 2023, 8(1), 5-11. doi: 10.11648/j.bmb.20230801.12

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AMA Style

Stéphanie Lynseh Carine Sita Bénao, Amana Métuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Pegdwende Abel Sorgho, Guigma Géraud Joël, et al. Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production. Biochem Mol Biol. 2023;8(1):5-11. doi: 10.11648/j.bmb.20230801.12

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@article{10.11648/j.bmb.20230801.12,
  author = {Stéphanie Lynseh Carine Sita Bénao and Amana Métuor Dabiré and Rahimatou Yasmine Wendkuni Tiemtoré and Pegdwende Abel Sorgho and Guigma Géraud Joël and Jacques Simporé},
  title = {Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production},
  journal = {Biochemistry and Molecular Biology},
  volume = {8},
  number = {1},
  pages = {5-11},
  doi = {10.11648/j.bmb.20230801.12},
  url = {https://doi.org/10.11648/j.bmb.20230801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230801.12},
  abstract = {The Increase in antibiotic resistance is a threat to the world health. Some resistant bacteria have the ability to transfer from animals to humans either through their stool or through their flesh. The spread and emergence of antibiotic resistance is therefore a public health problem. For this study, a total of 101 chickens were randomly selected from the busiest recreation areas of Ouagadougou and fresh chicken droppings taken directly from the animal's intestines were collected. Of the samples analyzed, 78.21% and 9.90% respectively contained Escherichia coli and Salmonella spp. For the 10 isolates of Salmonella spp, a resistance to CTX-M of 10%, 10% to CAZ, 10% to CRO and finally 70% resistant to AMC was observed. For the 79 strains of Escherichia coli, it was observed an absence of resistance to CTX-M, 3.79% to CAS, 3.79% to CRO and 84.81% of strain resistant to AMC. Four (4) mutil-resistant strains were identified and resistance genes were observed in 2 of these strains. The study revealed the presence of extended spectrum beta-lactamase genes in Enterobacteriaceae contained in chicken feces. A high consumption of flambé chickens infected or contaminated by these germs is then likely to increase the risk of development and spread of bacteria resistant to antibiotics in humans.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Prevalence of Escherichia coli and Salmonella spp Strains Isolated from Chicken Feces and Their Resistance to Antibiotics by Cefotaximase (CTX-M) Enzyme Production
AU  - Stéphanie Lynseh Carine Sita Bénao
AU  - Amana Métuor Dabiré
AU  - Rahimatou Yasmine Wendkuni Tiemtoré
AU  - Pegdwende Abel Sorgho
AU  - Guigma Géraud Joël
AU  - Jacques Simporé
Y1  - 2023/04/13
PY  - 2023
N1  - https://doi.org/10.11648/j.bmb.20230801.12
DO  - 10.11648/j.bmb.20230801.12
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 5
EP  - 11
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20230801.12
AB  - The Increase in antibiotic resistance is a threat to the world health. Some resistant bacteria have the ability to transfer from animals to humans either through their stool or through their flesh. The spread and emergence of antibiotic resistance is therefore a public health problem. For this study, a total of 101 chickens were randomly selected from the busiest recreation areas of Ouagadougou and fresh chicken droppings taken directly from the animal's intestines were collected. Of the samples analyzed, 78.21% and 9.90% respectively contained Escherichia coli and Salmonella spp. For the 10 isolates of Salmonella spp, a resistance to CTX-M of 10%, 10% to CAZ, 10% to CRO and finally 70% resistant to AMC was observed. For the 79 strains of Escherichia coli, it was observed an absence of resistance to CTX-M, 3.79% to CAS, 3.79% to CRO and 84.81% of strain resistant to AMC. Four (4) mutil-resistant strains were identified and resistance genes were observed in 2 of these strains. The study revealed the presence of extended spectrum beta-lactamase genes in Enterobacteriaceae contained in chicken feces. A high consumption of flambé chickens infected or contaminated by these germs is then likely to increase the risk of development and spread of bacteria resistant to antibiotics in humans.
VL  - 8
IS  - 1
ER  -

Copy | Download