Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation

Tongsu Ho; Haksong Pak; Sokjun Ri; Kang Kim; Namhyok Mun

doi:doi:10.11648/j.jps.20210905.14

| Peer-Reviewed

Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation

Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun

Published in Journal of Plant Sciences (Volume 9, Issue 5)

Received: 14 August 2021 Accepted: 22 September 2021 Published: 29 September 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.

Published in	Journal of Plant Sciences (Volume 9, Issue 5)
DOI	10.11648/j.jps.20210905.14
Page(s)	253-261
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Agrobacterium, miR393a, Transformation, Tuberous Begonia, Water Stress Tolerance

References

[1]	Abdeen A, Schnell J, Miki B (2010) Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3. BMC Genom 11: 69.
[2]	Akter A, Islam MM, Mondal SI, Mahmud Z, Jewel NA, Ferdous S (2014) Computational identification of miRNA and targets from expressed sequence tags of coffee (Coffea arabica). Saudi J Biol Sci 21: 3-12.
[3]	Anderson NO (2006) Flower Breeding and Genetics, Begonia, Dordrecht: Springer Netherlands, 241–275.
[4]	Axtell MJ (2013) Classification and comparison of small RNAs from plants. Annu Rev Plant Biol 64: 137–159.
[5]	Barrera-Figueroa BE, Gao L, Diop NN, Wu Z, Ehlers JD, Roberts PA (2011) Identification and comparative analysis of drought associated microRNAs in two cowpea genotypes. BMC Plant Biol 11: 127.
[6]	Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ (2005) Real–time quantifiation of microRNAs by stem–loop RT–PCR. Nucleic Acids Res 33: e179.
[7]	Dai XY, Xu YY, Ma QB, Xu WY, Wang T, Xue YB, Chong K (2007) Over-expression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought and salt stress in transgenic Arabidopsis. Plant Physiol 143: 1739–1751.
[8]	Dellaporta S, Wood J, Hicks J (1983) A plant DNA minipreparation, version II. Plant Mol Biol Rep 1: 19–21.
[9]	Dewitte A, Leus L, Eeckhaut T, Vanstechelman I, Van Huylenbroeck J, Van Bockstaele E (2009) Genome Size Variation in Begonia. Genome 52: 829–838.
[10]	Dongli H, Qiong W, Kun W, Pingfang Y (2015) Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination. PLoS ONE 12: 1–21.
[11]	Feng XM, You CX, Qiao Y, Mao K, Hao YJ (2010) Ectopic overexpression of Arabidopsis AtmiR393a gene changes auxin sensitivity and enhances salt resistance in tobacco. ACTA Physiol Plant 32: 997–1003.
[12]	Giannakoula A, Moustakas M, Mylona P, Papadakis I, Yupsanis T (2008) Aluminum tolerance in maize are correlated with increased levels of mineral nutrients, carbohydrates and proline, and decreased levels of lipid peroxidation and Al accumulation. J Plant Physiol 165: 385–396.
[13]	Gonzalez L, Gonzalez-Vilar M (2007) Determination of relative water content. Handbook of Plant Ecophysiology Techniques, 207–212.
[14]	Gu Y, Liu Y, Zhang J, Liu H, Hu Y, Du H (2013) Identification and characterization of microRNAs in the developing maize endosperm. Genomics, 102: 472–478.
[15]	Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4: 226–231.
[16]	Ho TS, Pak H, Ryem CK, Han MH (2019) Overexpression of OsmiR393a gene confers drought tolerance in creeping bentgrass. Plant Biotechnol Rep 13: 85–93.
[17]	Hu H, Xiong L (2014) Genetic engineering and breeding of drought resistant crops. Annu Rev Plant Biol 65: 715–741.
[18]	Jefferson RA, Kavanagh TA, Beven MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907.
[19]	Khan GA, Declerck M, Sorin C, Hartmann C, Crespi M, Lelandais-Briere C (2011) MicroRNAs as regulators of root development and architecture. Plant Mol Biol 77: 47–58.
[20]	Kiyokawa S, Kikuchi Y, Kamada H, Harada H (1996) Genetic transformation of Begonia×tuberhybrida by rol genes. Plant Cell Rep 15: 606–609.
[21]	Lichtenthaler HK, Wellburn AR (1985) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc T11: 591–592.
[22]	Liu SC, Xua YX, Ma JQ, Wang WW, Chen W, Huang DJ, Fang J, Li XJ, Chen L (2016) Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress, Physiol Plantarum 158: 435–451.
[23]	Liu T, Ye X, Li M, Li J, Qi H, Hu X (2020) H₂O₂ and NO are involved in trehalose-regulated oxidative stress tolerance in cold-stressed tomato plants, Environ Exp Bot 171: 103961.
[24]	Morran S, Eini O, Pyvovarenko T, Parent B, Singh R, Ismagul A, Eliby S, Shirley N, Langridge P, Lopato S (2011) Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors. Plant Biotechnol J 9: 230–249.
[25]	Ohki S, Hashimoto Y, Ohno M (2009) Preliminary Report on Agrobacterium-Mediated Genetic Transformation of Begonia rex and Saintpaulia Spp. Acta Hortic 829: 345–348.
[26]	Ornek H, Aysan Y, Mirik M, Sahin F (2007) First report of bacterial leaf spot caused by Xanthomonas axonopodis pv. begoniae, on begonia in Turkey. Plant Pathol 56: 347.
[27]	Pak H, Guo Y, Chen M, Chen K, Li Y, Hua S, Sums I, Meng H, Shi C, Jiang L (2009) The effect of exogenous methyl jasmonate on the ﬂowering time, ﬂoral organ morphology, and transcript levels of a group of genes implicated in the development of oilseed rape (Brassica napus L.) ﬂowers. Planta 231: 79-91.
[28]	Patel KG, Mandaliya VB, Mishra GP, Dobaria JR, Thankappan R (2016) Transgenic peanut overexpressing mtlD gene confers enhanced salinity stress tolerance via mannitol accumulation and differential antioxidative responses. Acta Physiol Plant 38: 181.
[29]	Prafull S, Nitin UK, Manoj M (2018) Stress-Inducible Galactinol Synthase of Chickpea (CaGolS) is implicated in Heat and Oxidative Stress Tolerance Through Reducing Stress-Induced Excessive Reactive Oxygen Species Accumulation. Plant Cell Physiol 59: 155–166.
[30]	Rustgi S, Boex-Fontvieille E, Reinbothe C, von Wettstein D, Reinbothe S (2018) The Complex World of Plant Protease Inhibitors: Insights into a Kunitz-Type Cysteine Protease Inhibitor of Arabidopsis thaliana. Communicative & Integrative Biology, 11: 1368599.
[31]	Shibata M (2008) Importance of genetic transformation in ornamental plant breeding. Plant Biotechnol 25: 3–8.
[32]	Tanaka Y, Katsumoto Y, Brugliera F, Mason J (2005) Genetic engineering in floriculture. Plant Cell Tiss Org 80: 1–24.
[33]	Tian X, Dixon M, Zheng Y (2012) Susceptibility of Various Potted Begonias to Fusarium foetens. Can J Plant Pathol 34: 248–254.
[34]	Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress promoter. Plant Cell Online 16: 2481–2498.
[35]	Waditee R, Bhuiyan NH, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T (2005) Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. P Natl Acad Sci USA 102: 1318–1323.
[36]	Vidal EA, Araus V, Lu C (2010) Nitrate-responsive miR393/AFB3 regulatory module controls root system architecture in Arabidopsis thaliana. P Natl Acad Sci USA 107: 4477–4482.
[37]	Vidal EA, Moyano TC, Riveras E, Contreras-López O, Gutiérrez RA (2013) Systems approaches map regulatory networks downstream of the auxin receptor AFB3 in the nitrate response of Arabidopsis thaliana roots. P Natl Acad Sci USA 110: 12840–12845.
[38]	Xiang L, Kuaifei X, Zhen L, Kunling C, Caixia G, Mingyong Z (2016) MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds. Sci Rep 6: 1–12.
[39]	Yamada M, Morishita H, Urano K, Shiozaki N, Yamaguchi-Shinozaki K, Shinozaki K, Yoshiba Y (2005) Effects of free proline accumulation in petunias under drought stress. J Exp Bot 56: 1975-1981.
[40]	Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57: 781–803.

Cite This Article

Plain Text BibTeX RIS

APA Style

Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. (2021). Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. Journal of Plant Sciences, 9(5), 253-261. https://doi.org/10.11648/j.jps.20210905.14

Copy | Download

ACS Style

Tongsu Ho; Haksong Pak; Sokjun Ri; Kang Kim; Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J. Plant Sci. 2021, 9(5), 253-261. doi: 10.11648/j.jps.20210905.14

Copy | Download

AMA Style

Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J Plant Sci. 2021;9(5):253-261. doi: 10.11648/j.jps.20210905.14

Copy | Download

@article{10.11648/j.jps.20210905.14,
  author = {Tongsu Ho and Haksong Pak and Sokjun Ri and Kang Kim and Namhyok Mun},
  title = {Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation},
  journal = {Journal of Plant Sciences},
  volume = {9},
  number = {5},
  pages = {253-261},
  doi = {10.11648/j.jps.20210905.14},
  url = {https://doi.org/10.11648/j.jps.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210905.14},
  abstract = {The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation
AU  - Tongsu Ho
AU  - Haksong Pak
AU  - Sokjun Ri
AU  - Kang Kim
AU  - Namhyok Mun
Y1  - 2021/09/29
PY  - 2021
N1  - https://doi.org/10.11648/j.jps.20210905.14
DO  - 10.11648/j.jps.20210905.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 253
EP  - 261
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20210905.14
AB  - The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.
VL  - 9
IS  - 5
ER  -

Copy | Download

Author Information

Tongsu Ho

Department of Genetics, Faculty of Life Science, Kim Il Sung University, Pyongyang, DPR of Korea
Haksong Pak

Department of Genetics, Faculty of Life Science, Kim Il Sung University, Pyongyang, DPR of Korea
Sokjun Ri

Department of Genetics, Faculty of Life Science, Kim Il Sung University, Pyongyang, DPR of Korea
Kang Kim

Department of Genetics, Faculty of Life Science, Kim Il Sung University, Pyongyang, DPR of Korea
Namhyok Mun

Department of Genetics, Faculty of Life Science, Kim Il Sung University, Pyongyang, DPR of Korea

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. (2021). Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. Journal of Plant Sciences, 9(5), 253-261. https://doi.org/10.11648/j.jps.20210905.14

Copy | Download

ACS Style

Tongsu Ho; Haksong Pak; Sokjun Ri; Kang Kim; Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J. Plant Sci. 2021, 9(5), 253-261. doi: 10.11648/j.jps.20210905.14

Copy | Download

AMA Style

Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J Plant Sci. 2021;9(5):253-261. doi: 10.11648/j.jps.20210905.14

Copy | Download

@article{10.11648/j.jps.20210905.14,
  author = {Tongsu Ho and Haksong Pak and Sokjun Ri and Kang Kim and Namhyok Mun},
  title = {Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation},
  journal = {Journal of Plant Sciences},
  volume = {9},
  number = {5},
  pages = {253-261},
  doi = {10.11648/j.jps.20210905.14},
  url = {https://doi.org/10.11648/j.jps.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210905.14},
  abstract = {The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation
AU  - Tongsu Ho
AU  - Haksong Pak
AU  - Sokjun Ri
AU  - Kang Kim
AU  - Namhyok Mun
Y1  - 2021/09/29
PY  - 2021
N1  - https://doi.org/10.11648/j.jps.20210905.14
DO  - 10.11648/j.jps.20210905.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 253
EP  - 261
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20210905.14
AB  - The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.
VL  - 9
IS  - 5
ER  -

Copy | Download