Establishment of Microfluidic Chip Method for Rapid Screening of <i>Tobacco Rattle Virus</i>

Wang Jiaying; Zhang Jihong; Xu Ying; Duan Weijun

doi:doi:10.11648/j.jps.20231101.14

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Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus

Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun

Published in Journal of Plant Sciences (Volume 11, Issue 1)

Received: 9 February 2023 Accepted: 24 February 2023 Published: 9 March 2023

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Abstract

Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known plant viruses. It is able to be transmitted by nematodes of the genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, a rapid screening method was established for inspection of TRV in seeds or other plant materials, combining microfluidic chip technique and fluorescence detection system. The particular region (located at 496-995) of TRV strain (accession number: KP100069.1) was adopted for primer design, method establishment and improvement. Through analysis, this method was proved to be specific for only detecting sample infected by TRV, while 7 other leaf materials infected by Tomato spot wilt virus (TSWV), Tobacco ringspot virus (TRSV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Lily mosaic virus (LMV), Tomato ringspot virus (ToRSV), and Prunus necrotic ringspot virus (PNRSV), respectively, showed negative results. Sensitivity tests represented a detection limit as low as 1.00×10²copies/μL which is beyond normal PCR assays. In one word, microfluidic chip based analyzing platforms hold high promises to enable high-throughput and high-precision screening with less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability, thus promoting the development of biosecurity diagnostics.

Published in	Journal of Plant Sciences (Volume 11, Issue 1)
DOI	10.11648/j.jps.20231101.14
Page(s)	22-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Tobacco rattle virus (TRV), Biosecurity, Diagnostic Technology, Microfluidic Chip

References

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APA Style

Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun. (2023). Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. Journal of Plant Sciences, 11(1), 22-27. https://doi.org/10.11648/j.jps.20231101.14

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ACS Style

Wang Jiaying; Zhang Jihong; Xu Ying; Duan Weijun. Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. J. Plant Sci. 2023, 11(1), 22-27. doi: 10.11648/j.jps.20231101.14

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AMA Style

Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun. Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. J Plant Sci. 2023;11(1):22-27. doi: 10.11648/j.jps.20231101.14

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@article{10.11648/j.jps.20231101.14,
  author = {Wang Jiaying and Zhang Jihong and Xu Ying and Duan Weijun},
  title = {Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {1},
  pages = {22-27},
  doi = {10.11648/j.jps.20231101.14},
  url = {https://doi.org/10.11648/j.jps.20231101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231101.14},
  abstract = {Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known plant viruses. It is able to be transmitted by nematodes of the genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, a rapid screening method was established for inspection of TRV in seeds or other plant materials, combining microfluidic chip technique and fluorescence detection system. The particular region (located at 496-995) of TRV strain (accession number: KP100069.1) was adopted for primer design, method establishment and improvement. Through analysis, this method was proved to be specific for only detecting sample infected by TRV, while 7 other leaf materials infected by Tomato spot wilt virus (TSWV), Tobacco ringspot virus (TRSV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Lily mosaic virus (LMV), Tomato ringspot virus (ToRSV), and Prunus necrotic ringspot virus (PNRSV), respectively, showed negative results. Sensitivity tests represented a detection limit as low as 1.00×102 copies/μL which is beyond normal PCR assays. In one word, microfluidic chip based analyzing platforms hold high promises to enable high-throughput and high-precision screening with less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability, thus promoting the development of biosecurity diagnostics.},
 year = {2023}
}

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TY  - JOUR
T1  - Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus
AU  - Wang Jiaying
AU  - Zhang Jihong
AU  - Xu Ying
AU  - Duan Weijun
Y1  - 2023/03/09
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231101.14
DO  - 10.11648/j.jps.20231101.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 22
EP  - 27
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231101.14
AB  - Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known plant viruses. It is able to be transmitted by nematodes of the genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, a rapid screening method was established for inspection of TRV in seeds or other plant materials, combining microfluidic chip technique and fluorescence detection system. The particular region (located at 496-995) of TRV strain (accession number: KP100069.1) was adopted for primer design, method establishment and improvement. Through analysis, this method was proved to be specific for only detecting sample infected by TRV, while 7 other leaf materials infected by Tomato spot wilt virus (TSWV), Tobacco ringspot virus (TRSV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Lily mosaic virus (LMV), Tomato ringspot virus (ToRSV), and Prunus necrotic ringspot virus (PNRSV), respectively, showed negative results. Sensitivity tests represented a detection limit as low as 1.00×102 copies/μL which is beyond normal PCR assays. In one word, microfluidic chip based analyzing platforms hold high promises to enable high-throughput and high-precision screening with less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability, thus promoting the development of biosecurity diagnostics.
VL  - 11
IS  - 1
ER  -

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Author Information

Wang Jiaying

Technical Center, Ningbo Customs, Ningbo, China
Zhang Jihong

Technical Center, Ningbo Customs, Ningbo, China
Xu Ying

Technical Center, Ningbo Customs, Ningbo, China
Duan Weijun

Technical Center, Ningbo Customs, Ningbo, China

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Plain Text BibTeX RIS

APA Style

Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun. (2023). Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. Journal of Plant Sciences, 11(1), 22-27. https://doi.org/10.11648/j.jps.20231101.14

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ACS Style

Wang Jiaying; Zhang Jihong; Xu Ying; Duan Weijun. Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. J. Plant Sci. 2023, 11(1), 22-27. doi: 10.11648/j.jps.20231101.14

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AMA Style

Wang Jiaying, Zhang Jihong, Xu Ying, Duan Weijun. Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus. J Plant Sci. 2023;11(1):22-27. doi: 10.11648/j.jps.20231101.14

Copy | Download

@article{10.11648/j.jps.20231101.14,
  author = {Wang Jiaying and Zhang Jihong and Xu Ying and Duan Weijun},
  title = {Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {1},
  pages = {22-27},
  doi = {10.11648/j.jps.20231101.14},
  url = {https://doi.org/10.11648/j.jps.20231101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231101.14},
  abstract = {Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known plant viruses. It is able to be transmitted by nematodes of the genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, a rapid screening method was established for inspection of TRV in seeds or other plant materials, combining microfluidic chip technique and fluorescence detection system. The particular region (located at 496-995) of TRV strain (accession number: KP100069.1) was adopted for primer design, method establishment and improvement. Through analysis, this method was proved to be specific for only detecting sample infected by TRV, while 7 other leaf materials infected by Tomato spot wilt virus (TSWV), Tobacco ringspot virus (TRSV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Lily mosaic virus (LMV), Tomato ringspot virus (ToRSV), and Prunus necrotic ringspot virus (PNRSV), respectively, showed negative results. Sensitivity tests represented a detection limit as low as 1.00×102 copies/μL which is beyond normal PCR assays. In one word, microfluidic chip based analyzing platforms hold high promises to enable high-throughput and high-precision screening with less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability, thus promoting the development of biosecurity diagnostics.},
 year = {2023}
}

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TY  - JOUR
T1  - Establishment of Microfluidic Chip Method for Rapid Screening of Tobacco Rattle Virus
AU  - Wang Jiaying
AU  - Zhang Jihong
AU  - Xu Ying
AU  - Duan Weijun
Y1  - 2023/03/09
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231101.14
DO  - 10.11648/j.jps.20231101.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 22
EP  - 27
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231101.14
AB  - Tobacco rattle virus (TRV), member of the genus Tobravirus, is an important plant pathogen with a wide host range beyond any known plant viruses. It is able to be transmitted by nematodes of the genera Trichodorus and Paratrichodorus (Trichodoridae). In this paper, a rapid screening method was established for inspection of TRV in seeds or other plant materials, combining microfluidic chip technique and fluorescence detection system. The particular region (located at 496-995) of TRV strain (accession number: KP100069.1) was adopted for primer design, method establishment and improvement. Through analysis, this method was proved to be specific for only detecting sample infected by TRV, while 7 other leaf materials infected by Tomato spot wilt virus (TSWV), Tobacco ringspot virus (TRSV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Lily mosaic virus (LMV), Tomato ringspot virus (ToRSV), and Prunus necrotic ringspot virus (PNRSV), respectively, showed negative results. Sensitivity tests represented a detection limit as low as 1.00×102 copies/μL which is beyond normal PCR assays. In one word, microfluidic chip based analyzing platforms hold high promises to enable high-throughput and high-precision screening with less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability, thus promoting the development of biosecurity diagnostics.
VL  - 11
IS  - 1
ER  -

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