Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P <0.05) against plant pathogens. Apply optimize formulation at 1% on the detached cocoa leaves before inoculation with Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.
| Published in | Journal of Plant Sciences (Volume 11, Issue 6) |
| DOI | 10.11648/j.jps.20231106.11 |
| Page(s) | 171-181 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Theobroma cocoa L., Phytophthora megakarya, Bioformulation of Fungicide, Neem, Sida Weed, Oyster Shell
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APA Style
Foka, E. T., Tayo, P. M. T., Pacha, T. F. M., Dzelamonyuy, A., Sado, S. L. K., et al. (2023). Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. Journal of Plant Sciences, 11(6), 171-181. https://doi.org/10.11648/j.jps.20231106.11
ACS Style
Foka, E. T.; Tayo, P. M. T.; Pacha, T. F. M.; Dzelamonyuy, A.; Sado, S. L. K., et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J. Plant Sci. 2023, 11(6), 171-181. doi: 10.11648/j.jps.20231106.11
AMA Style
Foka ET, Tayo PMT, Pacha TFM, Dzelamonyuy A, Sado SLK, et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J Plant Sci. 2023;11(6):171-181. doi: 10.11648/j.jps.20231106.11
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Download@article{10.11648/j.jps.20231106.11,
author = {Ebenezer Tatiekam Foka and Paul Martial Téné Tayo and Tatiana Flore Magni Pacha and Aristide Dzelamonyuy and Sylvain Leroy Kamdem Sado and Pierre Effa Onomo and Cécile Annie Ewané and Thaddée Boudjeko},
title = {Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya},
journal = {Journal of Plant Sciences},
volume = {11},
number = {6},
pages = {171-181},
doi = {10.11648/j.jps.20231106.11},
url = {https://doi.org/10.11648/j.jps.20231106.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231106.11},
abstract = {Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.
},
year = {2023}
}
TY - JOUR T1 - Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya AU - Ebenezer Tatiekam Foka AU - Paul Martial Téné Tayo AU - Tatiana Flore Magni Pacha AU - Aristide Dzelamonyuy AU - Sylvain Leroy Kamdem Sado AU - Pierre Effa Onomo AU - Cécile Annie Ewané AU - Thaddée Boudjeko Y1 - 2023/11/09 PY - 2023 N1 - https://doi.org/10.11648/j.jps.20231106.11 DO - 10.11648/j.jps.20231106.11 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 171 EP - 181 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20231106.11 AB - Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease. VL - 11 IS - 6 ER -
Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon
Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
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