Camel dung is an exceptionally abundant biomass resource with high organic matter content and rich in multiple nutritional elements. Using camel dung hydrolysate as a substrate for photosynthetic bacterial hydrogen production shows promising potential, but the bottleneck restricting this technology lies in the relatively low reducing sugar content in the hydrolysate. Optimizing hydrolysis process conditions is an effective solution to this issue, with the acid-enzyme hydrolysis method demonstrating significant advantages in camel dung hydrolysis. Building on this foundation, this experiment optimized the acid-enzyme hydrolysis process conditions for camel dung, selecting acid type, acid concentration, hydrolysis time, hydrolysis temperature, enzymatic pH, and enzymatic time as optimization parameters. The optimal hydrolysis conditions were determined as follows: hydrochloric acid as the optimal acid, sulfuric acid concentration of 7.0%, hydrolysis time of 30 min, hydrolysis temperature of 115°C, enzymatic pH of 5.0, and enzymatic time of 16 h. Under these conditions, the hydrolysis rate of camel dung reached 46.00 ± 0.97%, and the reducing sugar content in the hydrolysate reached 56.03 ± 1.25 g/L. Using the reducing sugar obtained from camel dung hydrolysis as a carbon source for photosynthetic bacterial hydrogen production not only alleviates the environmental burden caused by excessive camel dung accumulation but also reduces the cost of hydrogen production via photosynthetic bacteria. This approach helps mitigate energy pressure to some extent, promotes the livestock economy, and contributes to the sustainable development of the industry.

Camel Dung, Resource Utilization of Camel Dung, Optimization of Conditions